: Partial purification of phosphoenolpyruvate carboxykinase (PEPCK) from type 2 diabetic patients sera take place using some purification steps such as participation with ammonium sulphate (55-80%) and filtered through dialysis, then ion exchange chromatography by DEAE sepharose anion column, gel filtration chromatography by sephadex G-100 column. In ion exchange step, there are four peak are obtained, the highest enzyme activity obtained by (0.4 M Nacl) with purification fold (2.18), yield (44.3) of enzyme and specific activity (13.5) mg/ng, which obtained a single peak by gel filtration chromatography, the degree of purification (5.34) fold, yield of enzyme (20%) with specific activity (33.109mg/ng). The purified enzyme had an optimum temperature (37oC) and pH (7.4). Purified molecular weight was measured using sodium dodecyl sulfate and polyacrylamide gel (SDS.PAGE) electrophoresis showing approximately 64 KD of molecular weight.
PARTIAL PURIFICATION AND CHARACTERIZATION OF
PHOSPHOENOLPYRUVATE CARBOXYKINASE FROM SERUM OF TYPE 2
DIABETIC PATIENTS
Phosphoenolpyruvate
carboxykinase
diabetic type 2
Publication Date
Thu Aug 01 2019
Journal Name
Journal Of Economics And Administrative Sciences
مقارنة مقدرات بيز لدالة المعولية لتوزيع باريتو من النوع الاول باستعمال دوال معلوماتية مضاعفة مختلفة
Pareto type I distribution
reliability function
bayes method
double prior distributions (chi-gamma square distribution. gamma- erlang distribution
erlang exponential distribution
the squared error loss function.
توزيع باريتو من النوع الاول
دالة المعولية
طريقة بيز
الدوال المعلوماتية المضاعفة : توزيع مربع كاي- كاما
توزيع كاما - ارلنك
التوزيع ارلنك- الاسي
دالة الخسارة التربيعية.
The comparison of double informative priors which are assumed for the reliability function of Pareto type I distribution. To estimate the reliability function of Pareto type I distribution by using Bayes estimation, will be used two different kind of information in the Bayes estimation; two different priors have been selected for the parameter of Pareto type I distribution . Assuming distribution of three double prior’s chi- gamma squared distribution, gamma - erlang distribution, and erlang- exponential distribution as double priors. The results of the derivaties of these estimators under the squared error loss function with two different double priors. Using the simulation technique, to compare the performance for
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