Background: Removal of bacteria from the pulp system by instrumentation of an infected root canal, will be significantly reduced the number of bacteria, but it is well documented that instrumentation alone can-not clean and kill all bacteria found on the root canal walls. Antibacterial irrigants are needed to kill the remaining microorganisms. The aims of this study was to assess antibacterial effect of titanium tetrafluoride (TiF4) solution and brewing green tea against root canal bacteria and to compare with sodium hypochlorite and normal saline through microbiological and molecular studies. Materials and methods: Microbiological study was carried out to determine the concentration of titanium tetrafluoride and brewing green tea at which they exert antibacterial effect against ten swabs that had been taken from necrotic root canals that were incubated aerobically and anaerobically by paper disk diffusion test, whilemolecular study carried out among Forty children in which the antibacterial effect of titanium tetrafluoride and brewing green tea compared with sodium hypochlorite and normal saline were assessed by real time polymerase chain reaction using SYBR Green. Results: The microbiological study results showed that TiF4 achieved maximum antibacterial effect at concentration 5% against aerobic and anaerobic bacteria while green tea exhibited antibacterial effect when brewed for 20 minutes at concentration 100mg/1ml against staphylococcus aureus, but not active against other microorganisms like Escherichia coli and streptococcus. While results of molecular study illustrated that sodium hypochlorite remained the most effective endodontic irrigant solutions followed by titanium tetrafluoride then green tea while normal saline showed no antibacterial effect. Statistically titanium tetrafluoride, green tea and sodium hypochlorite have significant differences compared to normal saline. Conclusions: This study revealed that, titanium tetra fluoride and brewing green tea can be used as antibacterial irrigant solutions for root canal treatment in children.
Nicotine was separated from eggplant and green pepper seeds (Solanaceous) by High Performance Liquid Chromatography (HPLC).The concentration of nicotine in the eggplant extract (0.871-0.877 μg/ml) was determined by injecting standard material with 0.5 and 1.5 μg/ml, while the concentrations of nicotine in green pepper extract (0.613-0.618 μg/ml) was determined when the standard material was injected with 0.5 and 1.5 μg/ml. The qualitative chemical data was calculated from derivations of the standard material. Nicotine concentration was measured qualitatively in both extracts through the calibration curve and method of the standard addition. This technique has high accuracy and compatibility, bringing the proportion of relati
... Show MoreThe research aims at clarifying the role of green human resource management practices in achieving sustainable development. The research problem is that the health sector is less concerned with environmental aspects, specifically green human resource management practices, Which are reflected on sustainable development with their economic, environmental and social dimensions as well as reducing costs, waste minimization and recycling, And the research started from two main hypotheses to explore the correlation and influence between the variables of the research by analyzing the answers of the research sample, which included (136) employees of the Al-Imamein Al-kadhemein medical city, Data and information were collected using quest
... Show MoreWith the twenty - First century, It becomes clear to us that for 110 years the Japanese - Chinese relations have been witnessing big radical events and developments. ( we take the year of 1949 as the starting date for this relations). In order to prove hypothesis the study divided in to four chapters. - The first chapter deals with explaining the factors of the subject of the Japanese - Chinese relations which described as Indirect threat and aggressive relations from Japan to China after the Second World War by Japanese entering in the American world strategy, in order to destroy the communist china's system which decleard in 1949, because the united states understood that the new system in Beijing could threat American's Interest
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