The study was planned to evaluate the effect of adding Guanidinoacetic acid in some characteristics of the sperm of the rams. This study was conducted in the animal field of the Animal Production Department / Faculty of Agricultural Engineering Sciences / University of Baghdad, for the period from 5/8/2018 to 28/2/2019. In this experiment, 3 rams were used at the age of 2-2.5 years and weighed 50-54 kg. The semen was collected early in the morning and once a week and the semen was pooled to remove the individual differences. The treatments were divided: GAA-free control group, treatment T1 (0.05 mg / 100 ml GAA), T2 treatment (0.1 mg / 100 ml GAA) and T3 treatment (0.2 mg / 100 ml GAA). The results of the study showed a significant decrease in the concentration of malondialdehyde at time (0 and 72 hours) from cryopreservation in treatment T3 compared with the rest of the treatments. There were no significant differences between time-saving coefficients (0) in AST concentration. The AST concentration of time was reduced by 72 hours of conservation in T3 compared to control. ALT concentration was significantly reduced in T3 when conserved by time (0 and 72 hours) of cryopreservation compared with other treatments. The concentration of glutathione significantly increased at time T3 (0 and 72 h) from preservation of cooling. SOD concentration was significantly increased in T3 (0 and 72 hours) of conservation. There was no significant effect of GAA in creatine kinase activity throughout the experiment.
