Quorum sensing (QS) is a perfectly orchestrated molecular communication system. It is a boon for Klebsiella pneumoniae, and bane for the host. This system is believed to make K. pneumoniae a leading cause of multidrug-resistant (MDR) nosocomial infections. This study aimed to investigate the antibacterial and anti-biofilm potential of medicinal plant extracts through interfering with QS of K. pneumoniae. The effect of different concentrations of ethanolic extracts of cinnamon and clove on K. pneumoniae was determined by analyzing the growth curve, survival assay (MTT), Qualitative and quantitative biofilm formation, antibiotic resistance, along with studying gene expression of the genes encoding the above traits, using quantitative real time polymerase chain reaction (qRT-PCR). The low concentrations of the plant extracts did not affect neither on the bacterial growth, nor on the viability of K. pneumoniae, supported with growth curves. Additionally, the biofilm production was inhibited even by lower plant concentrations, and both cinnamon and clove extracts were able to render the MDR bacteria to be more susceptible to antibiotics. At the molecular levels, the bacterium treated with either clove or cinnamon or in combination showed under expression of the biofilm formation regulation gene (bssS), the carbapenem resistance gene (bla), as well as the QS target gene (LuxS). Both clove and cinnamon ethanolic extracts exerted potent impacts on reducing pathogenic traits regulated by QS in K. pneumoniae. Finally, the study recommends further exploration of clove and cinnamon extracts separately or in combination to develop alternative therapies against MDR K. pneumoniae infections.
The effect of number of pulses of pulsed laser on materials is studied analytically, different pulses has been used with the same delay time. The depth of possible damage to the surface of copper and titanium as well as depth of the crater to both materials were considered in this study. The study revealed that linear model is only possible when estimating depth of possible damage for copper material, this means that the depth of possible damage increases with the increment of number of laser pulses .As for titanium material, it is found the relationship is nonlinear. The depth of possible damage of titanium and copper is not the same, and copper seems to be more predictable than titanium.
Production logging is used to diagnose well production problems by evaluating the flow profile, entries of unwanted fluids and downhole flow regimes. Evaluating wells production performance can be easily induce from production logs through interpretation of production log data to provide velocity profile and contribution of each zone on total production. Production logging results supply information for reservoir modeling, provide data to optimize the productivity of existing wells and plan drilling and completion strategies for future wells. Production logging was carried out in a production oil well from Mishrif formation of West Qurna field, with the objective to determine the flow profile and fluid contributions from the perforations af
... Show MoreFour subsurface sections and electrical, porosity logs, and gamma-ray logs of the Khasib Formation (age Late Turonian-Lower Coniacian) were studied to identify reservoir characteristics and to evaluate the reservoir properties of the Khasib reservoir units in the East Baghdad oilfield. The lithology of the formation is limestone throughout the whole sequence in all studied wells EB-83, EB-87, EB-92, and EB94. It is bounded conformably from the top by Tanuma Formation and has a conformable lower contact with Kifl Formation. The lower and upper boundaries of the formation were determined using well log analysis, and the formation was divided into three main rock units (Kh1, Kh2, and Kh3), depending on the porosity logs. The porosi
... Show More
The study area is located in the south-eastern part of AL- Anbar province, between latitudes (31˚02ˉ00˭ -33˚03ˉ00˭) north and longitudes (40˚02ˉ00˭ -43˚04ˉ00˭) to the east. The research studied predictive mathematical Model of groundwater within Umm-Er Radhuma Formation and by (44) wells distributed randomly within the boundaries of the study area, all of them fall within the unconfined aquifer. Through the operating of wells on the time of (30) day and taking the operating results of each (5) days, results showed that the drawdown in groundwater levels values ranged from (13) cm after running the model for a period of 5 days and (120) cm after running Model for thirty days. And the drawdown values are concentrated near wells
... Show MoreProduction logging is used to diagnose well production problems by evaluating the flow profile, entries of unwanted fluids and downhole flow regimes. Evaluating wells production performance can be easily induce from production logs through interpretation of production log data to provide velocity profile and contribution of each zone on total production. Production logging results supply information for reservoir modeling, provide data to optimize the productivity of existing wells and plan drilling and completion strategies for future wells. Production logging was carried out in a production oil well from Mishrif formation of West Qurna field, with the objective to determine the flow profile and fluid contributions from the perforations
... Show MoreThe objective of this paper is determining the petrophysical properties of the Mauddud Formation (Albian-Early Turonian) in Ratawi Oil Field depending on the well logs data by using interactive petrophysical software IP (V4.5). We evaluated parameters of available logs that control the reservoir properties of the formation, including shale volume, effective porosity, and water saturation. Mauddud Formation is divided into five units, which are distinguished by various reservoir characteristics. These units are A, B, C, D, and E. Through analyzing results of the computer processed interpretation (CPI) of available wells, we observed that the main reservoir units are B and D, being distinguished by elevated values of eff
... Show More
(5)
Portland Cement is manufactured by adding 3% gypsum to clinker which is produced by grinding, pulverizing, mixing, and then burning a raw mix of silica, and calcium carbonate. Limestone is the main source of carbonates, while clay collected from arable land is the main source of silica. The marl in the Euphrates Formation was studied as an alternative to arable lands. Nine boreholes drilled and penetrated the marl layer in selected locations at the Kufa cement quarry. Forty-one samples of marl from boreholes and four samples of limestone from the closed area were collected. The chemical content of the major oxides and the hardness of the marl layer was very encouraging as a raw material for Portland Cement as they are SiO2 (17.60),
... Show More
(2)
Production logging is used to diagnose well production problems by evaluating the flow profile, entries of unwanted fluids and downhole flow regimes. Evaluating wells production performance can be easily induce from production logs through interpretation of production log data to provide velocity profile and contribution of each zone on total production. Production logging results supply information for reservoir modeling, provide data to optimize the productivity of existing wells and plan drilling and completion strategies for future wells. Production logging was carried out in a production oil well from Mishrif formation of West Qurna field, with the objective to determine the flow profile and fluid contributions from the perforations af
... Show MoreCeramic coating compose from a ceramic mixture (MgO, Al2O3) and metall (Al-Ni) were produced by Thermal Spray Technique. The mixed ratio of used materials Al:Ni (50%) and 40% of Al2O3 and 10% MgO. This mixture was spray on a stainless steel substrate of type (316 L) by using thermal spray with flame method and at spraying distances (8, 12, 16 and 20) cm, then the prepared films were treated by laser and thermal treatment. After that performing a hardness and adhesion tests were eximined. The present study shows that the best value of the thermal treatment is 1000 ℃ for 30 mint; the optimum spray distance is 12 cm and most suitable laser is 500 mJ where the microscopic and mechanical character
... Show More
(1)