Quorum sensing (QS) is a perfectly orchestrated molecular communication system. It is a boon for Klebsiella pneumoniae, and bane for the host. This system is believed to make K. pneumoniae a leading cause of multidrug-resistant (MDR) nosocomial infections. This study aimed to investigate the antibacterial and anti-biofilm potential of medicinal plant extracts through interfering with QS of K. pneumoniae. The effect of different concentrations of ethanolic extracts of cinnamon and clove on K. pneumoniae was determined by analyzing the growth curve, survival assay (MTT), Qualitative and quantitative biofilm formation, antibiotic resistance, along with studying gene expression of the genes encoding the above traits, using quantitative real time polymerase chain reaction (qRT-PCR). The low concentrations of the plant extracts did not affect neither on the bacterial growth, nor on the viability of K. pneumoniae, supported with growth curves. Additionally, the biofilm production was inhibited even by lower plant concentrations, and both cinnamon and clove extracts were able to render the MDR bacteria to be more susceptible to antibiotics. At the molecular levels, the bacterium treated with either clove or cinnamon or in combination showed under expression of the biofilm formation regulation gene (bssS), the carbapenem resistance gene (bla), as well as the QS target gene (LuxS). Both clove and cinnamon ethanolic extracts exerted potent impacts on reducing pathogenic traits regulated by QS in K. pneumoniae. Finally, the study recommends further exploration of clove and cinnamon extracts separately or in combination to develop alternative therapies against MDR K. pneumoniae infections.
Toxoplasma gondii is an opportunistic pathogen in which the reactivation of a latent infection can cause death in congenitally infected fetuses, newborns, and immunocompromised patients. This study aimed to determine the seropositivity of toxoplasmosis infection and the possible association with Interleukin-12 (IL-12) and Interleukin-23 (IL-23) cytokines in breast cancer patients. In this study, 190 women were enrolled. All serum samples were tested for T. gondii immunoglobulins (IgG and IgM) antibodies and (IL-12, IL-23) levels using ELISA technique. The result of this study showed that breast cancer patients recorded the highest percentage of toxoplasmosis infection. There were no positivity rates for anti- Toxopl
... Show MoreSTAG3 is the meiotic component of cohesin and a member of the Cancer Testis Antigen (CTA) family. This gene has been found to be overexpressed in many types of cancer, and recently, its variants have been implicated in other disorders and many human diseases. Therefore, this study aimed to analyze the major variants of STAG3. Western blot (WB) and immunoprecipitation (IP) assays were performed using two different anti-STAG3 antibodies that targeted the relevant protein in MCF-7, T-47D, MDA-MB-468, and MDA-MB-231 breast cancer cells with Jurkat and MCF-10A cells as positive and negative controls, respectively. In silico analyses were searched to study the major isoforms. WB and IP assays revealed two abundant polypeptides < 191 kDa and
... Show MoreThis researchs the preparation of particulate polymer composites from Alkyd resin and Iraqi Burn Kaolin which were added as (20%,30%,40%,50%)and comparing with the polymer. It studied Thermal conductivity and Dielectric strength for both of the Alkyd resin and the Composite Material. The result showed an increase in Dielectric strength after adding the Iraqi Burn Kaolin , also the Thermal conductivity was increased by adding the Iraqi Burn Kaolin .
In this research, we use fuzzy nonparametric methods based on some smoothing techniques, were applied to real data on the Iraqi stock market especially the data about Baghdad company for soft drinks for the year (2016) for the period (1/1/2016-31/12/2016) .A sample of (148) observations was obtained in order to construct a model of the relationship between the stock prices (Low, high, modal) and the traded value by comparing the results of the criterion (G.O.F.) for three techniques , we note that the lowest value for this criterion was for the K-Nearest Neighbor at Gaussian function .
This work involves the preparation of the ligand [KL] :- ÂÂÂÂÂÂ
K[4-(N-(5-methylisoxazol-3-yl) sulfamyl) phenylcarbamodithioate] from the reaction of sulfamethoxazole with Carbon disulfide in the presence of potassium hydroxide under reflux (4 hours) using methanol as asolvent. The prepared ligand was characterized using FT-IR, UV-Vis, 1H,13C–NMR spectroscopy, molar conductivity and melting point, Complexes for the above ligand [KL] with some bivalent transition and non-transition metals (Mn +2, Co+2 , Ni+2 ,
... Show MoreLate Paleocene –Early Eocene algae and benthic foraminifera were identified
from the Sinjar Formation that crop out in three selected section from northern
Iraq. The selected sections: (Derbandikhan ,Qara dagh, Wara Mali has been
measured in the field and closely sampled to undertake details. A number of (131)
samples were collected illustrating all the lithological changes along the sections,
The limestone in the studied section is rich in algae and large benthic foraminifera,
The investigation of the thin sections allowed us to identify the (47 ) species of
foraminifera and (34) species of algae , Five biozones were distinguished for algae
which are :a- Amphiroa iraquensis range zone ,b- Trinocladus perplexus
This Study aims to engineering geological evaluation of Fatha and Injana Formations in northern Hamrin anticline throughout geological and engineering geological Survey including Preparing engineering geological map (Scale1:90000) and Studying rock Slopes Stability in (6) Stations ,representing all types of failures (taken place and possible).General Survey for rock Slopes included Classification and engineering description according to [1], [2]. The slopes in the area are Classified based on the direction of the Strike Slopes and Strike of beds into Parallel, Oblique Lateral and orthogonal Slopes according to [3].Classification and the Slope Types are Concordant Slope and discordant Slope . The rocks in the area consists of two engineer
... Show MoreGas Chromatography GC, Gas Chromatography–Mass spectrometry GC/MS techniques used for analysis of the crude oils that taken from (10) producing wells in Nasiriyah oil field including (NS-1, NS-3, NS-4, NS-5, NS-6, NS-7, NS-8, NS-9, NS-10, and NS-12) from Mishrif reservoir . This reservoir is one of the important reservoirs in Al-Nasiriyah oil field, and it will be the main subject in the current study in order to provide information of crude oil analysis in this area, also to provide information on its characterizations. Mishrif Formation is one of the principle carbonate reservoir in central and southern Iraq. It is part of the wasia group and widespread throughout the Arabian gulf, It is deposited during Cenomanian-Early Turonian cyc
... Show MoreThe current research is concerned with methods of formation and their effect on the sintering process of ceramic materials. The research is divided into a number of chapters. The first chapter addressed the research structure (the research problem, importance, objective, limits, and it also defined the terms used in the research). The second chapter addressed the theoretical framework, where the theoretical framework has been divided into three sections. The first section dealt with methods of formation of ceramic materials including: Plasticizing method 2- semi-dry pressing method 3- dry pressing method 4- extrusion method 5- casting method.
The researcher found that there is a clear difference between the methods through her formati