Background: One of the most dominant bacterial infections is urinary tract infection (UTI), both in the community and in the hospital settings Since drug resistance become one of the predominant problems of health worldwide, it is necessary to use new methods to overcome drug-resistant bacteria. In this regard, medicinal plants (Medicago sativa ) are considered one of the richest sources to produce antibiotics.
Aim of the study: evaluate the antibacterial effect of the fraction of ethyl acetate of Medicago Sativa extract against Escherichia Coli isolated from urine samples of patients with urinary tract infections.
Material and methods:
... Show MoreThe purpose of this study was to investigate the bacterial etiology of urinary tract infections microbiologic properties of Escherichia coli isolated from urinary tract infection patients against nine amoxicillin antibiotic. E.coli isolates were collected from patients samples suffering from urinary tract infection, based on biochemical tests of Epi 20 system .Nine Amoxicillin antibiotics were selected (some vials and other are capsules) which manufactured in different countries were bought from local pharmacies in Baghdad, for the purpose of knowing the inhibitory activity of these antibiotics on E.coli one of the main microorganisms to cause urinary tract infection, the antibiotics were prepared in a concentration of 100mg/ml and their
... Show MoreThe present study included a collecting of 165 specimens form different sources,
93 isolates were identified as Escherichia coli depending on morphological and
biochemical tests in addition to automated systems such as VITEK 2 and api 20E.
All isolates under study developed high resistance toward cefotaxime, ceftazidime,
ceftriaxone, and ciprofloxacin estimated by minimum inhibitory concentration. Stool
and wound specimens characterized by harbouring the highest resistant isolates in a
percentage reached 100% against antibiotics under study. Insignificant differences
were found between isolates collected from males and females. Upon using disk
displacement method to detect extended spectrum beta lactamases (ESBL),
Thirty uropathogenic E. coli isolates were isolated from hospitalized and non hospitalized patients, complaining of urinary tract infections, of Al-Kadhymia Teaching Hospital and subjected to tRNA extraction. A method of tRNA extraction was modified by adding sodium dodecyl sulfate (SDS) instead of urea. Polyacrylamide gel electrophoresis and two methods of staining, ethidium bromide staining and silver staining, as well as spectrophotometric detection were used.
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Twenty-seven S. aureus isolates were obtained from patients referring various hospitals in Baghdad. Only 17 isolates produced DNase. SNase was extracted and purified from Saphylococcus aureus 3 isolate since it produces the largest zone of clearance on DNase agar. Nevertheless, only those phenotypically-producer of DNase harboured nuc gene. Present study revealed that the crude enzyme had a specific activity of 50.66 unit/mg; while it reached 241 unit/mg after ion exchange chromatography using carboxymethyl cellulose column. SDS-PAGE showed a single sharp band with an approximately 16.8 kDa molecular weight. A matter indicates that the enzyme is consistently pure. Results proved that SNase was able to significantly (P< 0.05) reduce th
... Show MoreThree hospitals were chosen for the present (Maternity hospital, Raperin hospital and Rhizgari hospital) survey within Erbil city, 36 water samples were collected at regular monthly interval periods beginning at January to December 2012. Microbial analysis was done by selective medium and biochemical tests and the isolated bacteria from those hospitals were Eshcerichia coli, Acinetobacter lowffii, Klebsiella pneumoniae, Moraxilla spp., Salmonella Typhi, Citrbtobacter freundii, Vibrio fluvials, Acinetobacter haemolyticus, Weeksella zoohelcum, Pasteurella multicida, and Pseudomonas aeroginosa. E. coli isolates were subjected to antimicrobial susceptibility testing. In vitro activities of 10 different antibiotics against E. coli isolates we
... Show MoreThis study aimed to collected 150 feces sample from calves suffering sever diarrhea ,then there were isolation and purification of Cryptosporidium parvum oocyst from samples contain it. These oocysts were used to induce experimental infection in the Immunosupperessed mice, then the mice treated by Bifidium bacteria , its supernatant and local yogurt were used for bacterial isolation.
This study showed that the local yogurt was the best treatment which led to stop oocyst shedding from the mice in the 8th day after treatment under sufficient treatment (89.72%) , at the same time the mice treated with bacteria stopped the oocyst shedd
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