he study aimed to purify of Leucine aminopeptidase (LAP) from sera of hyperthyroidism patients and its relation to some thyroid hormones (TSH, T 3 , T 4 ) of subclinical hyperthyroidism and hyperthyroidism patients with lipid peroxidation levels that may be play a role in this diseases. Specimens were collected during the time from Nov 2017 until Jan 2018 from Endocrine and Diabetes Center, blood samples were collected from fifty healthy control and one hundred patients, patients were divided into two groups consisted of (50) with hyperthyroidism and (50) with subclinical hyperthyroidism. The aged for all subjects ranged (15-60) years with body mass ranged ((25- 29) kg/m 2 . The purification is done by addition of ammonium sulfate, dialysis, ion exchange chromatography by using DEAE -Sepharose and gel filtration chromatography by using Sepharose 6B. FT3, FT4, TSH, LAP, peroxynitrite, malondialdehyde were determined. Results showed the precipitate and concentrated protein appeared five peaks after eluted with Tris- HCl (50 mM, pH 7.0 in ion exchange column, LAP located in the first peak (wash fraction) and the second peak (elution fraction eluted with 0.1 M NaCl), also, results showed single peak for both first and second peaks after eluted in gel filtration chromatography following steps by using sodium dodecyl sulfate-polyacrylamide gel. As well as results showed a non- significant elevation in BM, FT 3 and FT 4 in subclinical-hyperthyroidism group when compared to the control group. And a highly significant elevation in LAP, peroxynitrite and MDA levels in the patient group compared to the control group, while a highly significant decrease in TSH levels was found in the patient group compared to the control group. Results showed a non-significant elevation in BMI in hyperthyroidism group when compared to the control group., also, showed a highly significant elevation in FT3, FT4 LAP, peroxynitrite and MDA levels in the patient group compared to the control group, while a highly significant decrease in TSH levels was found in the patient group compared to the control group. From this study, it is concluded Leucine aminopeptidase which partial purification from sera of hyperthyroidism patients by ion exchange chromatography and gel filtration chromatography have two isoenzymes ,also, concluded a highly significant elevation in LAP, peroxynitrite and MDA levels in the patient group compared to the control group , in addition to their correlation with thyroid hormone in the patient group, compared to the control group indicate that these parameters have an effect on these diseases.
A new chelate polymer (2-5-hydroxy-3-methyl-2- (3-nonyl benzene) imino) methyl) benzyl) 4-6-dimethyphenol] (K4) was prepared by using the condensation reaction method and identified by several techniques, including FT-IR, NMR, and atomic absorption spectroscopy, as well as TG-DTA thermal analysis. The kinetic equilibrium for the sorption of lead and cadmium ions on the chelate polymer surface was also investigated. The results showed that the sorption of both ions followed the pseudo-first-order and pseudo-second-order kinetic equilibrium. The rate constant values of pseudo-first-order reaction were equal to 0.062 and 0.057 min-1 , while the values of pseudo-second-order were 0.0103 and 0.053 L.m
... Show MoreThis study included the effect of chronic exposure of different concentrations of sodium chloride on the freshwater Crustacean Daphinea pulex from the order of Cladocera. The concentrations used for the chronic exposure were( 0.1‰, 0.5‰, 1‰, 1.5‰, 2‰ )to investigate the effect on the some reproductive parameter which included mean number of eggs and juveniles produced per female and per clutch,and mean number of clutch per female which decline from (6.0) clutch/female in the control treatment to (3.0) clutch/female in (2‰) concentrations, as well as to investigate the number of females produce the clutches and we found only one female produced six cluthes in the( 2‰) concentration of sodium chlor
... Show MoreToxoplasmosis is a widespread infection usually caused by Toxoplasma gondii (T. gondii) parasite. It occurs in humans and other warm blooded animals, causing severe problems. It was found that there is an alteration in the trace elements concentrations levels associated with some human diseases. This study aimed to investigate the changes in the concentrations of some trace elements (Mg, Fe , Zn, and Cu) in the sera of 60 immunocompetent patients with chronic toxoplasmosis and 82 healthy individuals as a control group. Measuring the serum level of seropositivity rate of anti-T. gondii antibodies was done by Enzyme Linked Immunosorbent Assay (ELISA) Kit, while the concentrations of trace elements were measured by absorption spectrophotometry
... Show MoreOne hundred thirty four urine samples were collected from diabetic patients during the period from March –November -2014. From these samples there were 62.7% positive samples, women represented 45.3% and men represented 54.7% , some of them were a symptomatic as women represented 60.5% and men represented 58.7% and some were with clinical symptoms with or without a history of UTI. Hypertension diabetic women represented 45% and men represented 26% .Men suffering from prostate enlargement were represented 35% from the total diabetics .The E.coli isolates were represented 28.6% from the total cases ,Klebsiella spp isolates represented 20%, S. aureus isolates represented 16.7%,P
... Show MoreAnemia in pregnancy can considerably elevate the maternal mortality risk and can negatively distress the development of fetus.
To assess the association between neonatal outcomes and maternal anemia (MA) among pregnant women (PW).
The present work is a clinical prospective one performed at Al-Elwiya Maternity
In the petroleum industry, multiphase flow dynamics within the tubing string have gained significant attention due to associated challenges. Accurately predicting pressure drops and wellbore pressures is crucial for the effective modeling of vertical lift performance (VLP). This study focuses on predicting the multiphase flow behavior in four wells located in the Faihaa oil field in southern Iraq, utilizing PIPESIM software. The process of selecting the most appropriate multiphase correlation was performed by utilizing production test data to construct a comprehensive survey data catalog. Subsequently, the results were compared with the correlations available within the PIPESIM software. The outcomes reveal that the Hagedorn and Brown (H
... Show MoreIn this paper, we will focus to one of the recent applications of PU-algebras in the coding theory, namely the construction of codes by soft sets PU-valued functions. First, we shall introduce the notion of soft sets PU-valued functions on PU-algebra and investigate some of its related properties.Moreover, the codes generated by a soft sets PU-valued function are constructed and several examples are given. Furthermore, example with graphs of binary block code constructed from a soft sets PU-valued function is constructed.
This study is at the heart of the pragmatic theory, as this research seeks to highlight the concept of necessity, and what the researchers have tended to do is that the development that has acquired integrated pragmatics is only for the development of the subject of necessity, as it is one of the contents of the saying, and this is what made Decroux define it as the semantic element that Concerning the saying, as for Grace; He paid great attention to the conversational imperative, and understood the requirement by clues or suggestions, as it is an element known for its absence and presence in the linguistic structures included in the text when reading or when understanding. This study has paved the way for clarifying the concept of necessi
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