The reason for conducting this study is to prolong release of miconazole in the ocular site of action by ocular-based gels (OBGs) formulations. The formulation factors affecting on the release from OBG should be studied using various gelling agents in various concentrations to achieve the improvement in retention and residence time in response to prolonged release. In this study, the formulations were prepared using carbopol 940, pectin, sodium alginate, poloxamer 407, and poly(methacrylic acid) at 0.5%, 1%, and 1.5% w/v, respectively. Hydroxypropyl methylcellulose E5 (HPMC E5) 1% was added as thickening agent/viscosity builder. The formulation containing carbopol 940, pectin and sodium alginate at 1.5% w/v, displayed a noticable improvement in viscosity, gelling capacity, and extended release for 7 h in comparison with the reference drug. Overall, the release showed that the sodium alginate with HPMC E5 form
Background: Estimation of the in situ expression of Fas and Fas ligand (FasL) in aborted women compared with normal human pregnancy .
Patients and Methods: A technique utilizing in situ hybridization(ISH) was performed to detect and determine the in situ expression of Fas and Fas ligand (FasL) mRNA using paraffin embedded sections of curettage samples obtained from 42 women, who were divided into two groups: 30 women with first trimester abortion and 12 women with induced abortion as control.
Results: The levels of the in situ expression of both Fas and Fas ligand (FasL) mRNA were found to be highly significant increased in group 1 as compared with group 2
(p<0.01), with a significant positive correla
In this work, combining acyclovir with ciprofloxacin (CCP), a new Schiff base and its metal complexes with iron (III), manganese (II), copper (II), zinc (II), and calcium (II) ions are synthesized and structurally characterized by XRD, SEM, 1H NMR, 13C NMR, FT-IR, and UV-Visible spectral techniques. Furthermore, molecular docking studies were carried out, and the complexes were tested to resolve any potential hang-ups in opposition to the Herpes virus and DFT calculation studies. In addition, the metal complexes and ligand were screened against antibacterial strains of one gram-positive bacteria (Staphylococcus aureus) and three gram-negative bacteria (
... Show MoreA protocol has been developed for micropropagation of Arachis hypogea L. under in
vitro conditions. Nodal explants gave rise to multiple shoots when cultured on MS medium
supplemented with different concentrations of BA (benzyladenine) with Kin (Kinetin) or GA3
(gibberellic acid). The highest response of shoot multiplication was obtained in MS
containing 1.5 mg.l¯¹ BA and 0.5 mg.L¯¹ Kin. The regenerated shootlets were rooted on MS
(Murashige and Skoog) basal medium with different concentrations of IBA (indolbutyric
acid) and IAA (indol acetic acid). The highest response of rooting was achieved with IBA at
0.05 + IAA at 0.05 mg.L¯¹. The maximum frequency of rooting and highest number of roots
Many trials were made to prepare Tinidazole 2% as bioadhesive vaginal gels using different gel bases including hydroxypropyl methyl cellulose (3 and 4% w/w), methylcellulose (3 and 4%w/w) and carboxymethylcellulose (2 and 3% w/w) .Swelling index of the polymers,pH , viscosity , bioadhesive force , and in-vitro drug release to the simulating vaginal fluid (S.V.F.) were investigated for all the prepared bioadhesive gels . The mechanism of drug release from the gel bases was also investigated.
The results revealed that C MC 3% gave the highest viscosity and bioadhesive strength with the lowest release rate while lowest viscosity and bioadhesive force
... Show MoreFlower samples of Calendula officinalis were collected from the gardens of the college of Pharmacy in Baghdad city to examine their antibacterial activity .The effect of ethanol crude extract of petals and reproductive parts of flowers in different concentrations were obtained and bioassayed in vitro for its bioactivity to inhibit the growth of eight types of bacteria .The extracts of petals part were clearly superior for all bacteria especially the bacteria Pseudomonas aeruginosa (inhibition zone was 25mm in the concentration 100mg/ml) from gram negative bacteria, and Staphylococcus aureus (inhibition zone was 14mm in the concentration 50mg/ml) from gram positive bacteria, whi
... Show MoreAnastatica hierochuntica L. is distributed throughout Arabain Peninsula, and elsewhere it is locally called "Kuffe Maryam" .All parts of the plant are used in folk medicine. This study amid to investigate the effect of aqueous extract of anastatica hierochunctica L. on the cancer cell lines AMN-3. Anti cancer activity of aqueous extract of anastatica hierochunctica L. showed anticancer activity against AMN-3 cell line for twelve concentrations (0.04, 0.09, 0.195, 0.39, 0.78, 1.56, 3.125, 6.25, 12.5, 25, 50, 100) mg/mL in comparison with negative control.
Anastatica hierochuntica L. is distributed throughout Arabain Peninsula, and elsewhere it is locally called "Kuffe Maryam" .All parts of the plant are used in folk medicine. This study amid to investigate the effect of aqueous extract of anastatica hierochunctica L. on the cancer cell lines AMN-3. Anti cancer activity of aqueous extract of anastatica hierochunctica L. showed anticancer activity against AMN-3 cell line for twelve concentrations (0.04, 0.09, 0.195, 0.39, 0.78, 1.56, 3.125, 6.25, 12.5, 25, 50, 100) mg/mL in comparison with negative control.
Cutaneous leishmaniasis (CL) also known under local names like (tropical sore, oriental sore and Baghdad sore) is the most common form of leishmaniasis. It is a public health and a social problem in many developing countries. The Old World disease primarily is caused by Leishmania major in dry desert areas and Leishmania tropica in urban areas. Recently, metal oxide nanoparticales have been versatile platforms for biomedical applications and therapeutic interventions. There is an urgent need to develop new types of antileishmanial agents instead of classical drug (pentostam), especially, when its efficacy showed a decline towards some strains of Leishmania. Therefore, the present work was aimed to evaluating antileishmanial activity of z
... Show MoreThe technique of plant tissue culture has been used to In vitro micropropagation of Spilanthes acmella (L.) Murr. It is an ornamental and medicinal plant not cultivated in Iraq. Seeds were sterilized and cultuared on full strength Murashige and Skoog medium(1962)(MS). Naphthalene acetic acid (NAA), 6- furfuryl aminopurine (Kin.) growth regulators were used at the Initiation stage.The combination between IAA and Kin. was used in multiplication stage. IAA was used for rooting the shoots. Results showed that 1.5% sodium hypochlorite for 15 min was very effective for disinfecting and survival. Nodes exhibited relatively highest response as compared with apical meristems and leaflets culture. Supplying the culture medium with 1 mg/L.
... Show MoreBackground: With the increased in the demands of adult orthodontics, the challenge of direct bonding to non-enamel surface (zirconium) had been increased. The present study was carried out to compare the shear bond strength of three different brackets (stainless steel, sapphire and composite) bonded to zirconium surface and study the mode of bond failure. Materials and methods: The sample was comprised of 30 models (8mm *6mm*1.5mm) of full contour zirconium veneers. They were divided into three groups according to the brackets type; all samples were treated first by sandblast with aluminum oxide particle 50 µm then coated by z-prime plus primer. A central incisor bracket of each group was bonded to the prepared zirconium surface with lig
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