The aim of the present study was to isolated the Enterococcus spp. from milk samples of cow and vaginal swabs from aborted women and patient women in Baghdad during September 2016 to april 2017. All 100 milk sample collecting was carried out on California Mastitis Test (CMT) and the positive Percentage of CMT reactions was 5% and the percentage of Enterococcus isolates from mastitic milk was 60% and 30% from nonmasitic milk. The prevalence of Enterococcus spp was 31% of milk samples and the prevalence of Enterococcus spp. Isolates were 67.74% of the isolates of cow milk samples were Enterococcus faecalis, 25.80% was Group D and 6.45% was non groupable while Enterococcus spp. isolates from aborted women samples were 20% and all isolated was Enterococcus faecalis. Enterococcus spp was identified by Lancefield grouping test, biochemical tests. This was accomplished by the collection of 200 sample of bovine milk and vaginal swab of aborted women the samples growth in Todd Hewitt broth and incubated aerobically for 24 hours at 37C then cultured on azide blood agar by using selective and differential media like macConkey and tellurite along with Lancefield grouping kit.Antibiotic sensitivity test has been done for some isolate which reflected high resistant to (vancomycin, pencillin, Ofloxacin, ciprofloxacin, nitrofurantoin, tetracycline and amikacin) the percentage of resistant to antibiotic was 100% in amikacin, nitrofurantoin and tetracycline for all isolated from aborted women and milk samples.