Imidacloprid (1-[(6-chloro-3-pyridinyl) methyl]-N-nitro-2-imidazolidinimine), is a recent systemic and contact insecticide with high activity against a wide range of pests. Continuous dispersion of this pesticide in the environment and its stability in soil results in environmental pollution which demands remediation. Used in this research Rhizobium pusense strain IHB 1 (OP218458.1) and Psychrobacter celer strain IHB2(OP672320.1). which were previously isolated from botanical fields soil of greenhouses which has been using imidacloprid pesticides for many years to determine optimum condition and degradation ability for imidacloprid by tested in minimal salt medium (MSM) for a duration of 21 days. The temperature, pH number, and concentration of the pesticide were determined for the growth of bacteria. The best growth of Psychrobacter celer strain IHB2(OP672320.1) was at 28°C, pH 6, and pesticide concentration 50ppm, while Rhizobium pusense strain IHB1 (OP218458.1) had the best growth at 24°C, pH 7, and pesticide concentration 75ppm.Levels of imidacloprid in MSM medium were analyzed by high-performance liquid chromatography (HPLC). Rhizobium pusense strain IHB 1 (OP218458.1) was able to degrade 50.2% and Psychrobacter celer strain IHB2(OP672320.1) was able to degrade 59.01% of the initial amount of imidacloprid at the concentration of 25 mg /L in MSM media. All bacteria introduced in this study were among the first reports of imidacloprid degrading isolates in MSM-limited media from greenhouse soil. Therefore, the results of this study demonstrate the effectiveness of using soil bacteria for microbial degradation of imidacloprid. These findings suggest that these strains may be promising candidates for bioremediation of imidacloprid-contaminated soils.
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An experiment was carried out in the vegetables field of Horticulture Department / College of Agriculture / Baghdad University , for the three seasons : spring and Autumn of 2005 , and spring of 2007 , to study the type of gene action in some traits of vegetative and flowery growth in summer squash crosses (4 x 3 = cross 1 , 3 x 7 = cross 2 , 3 x 4 = cross 3 , 3 x 5 = cross 4 , 5 x 1 = cross 5 , 5 x 2 = cross 6). The study followed generation mean analysis method which included to each cross (P1 , P2 , F1 , F2 , Bc1P1 , Bc1P2) , and those populations obtained by hybridization during the first and second seasons. Experimental comparison was performed in the second (Two crosses only) and third seasons , (four crosses) by using RCBD with three
... Show MoreMixture experiments are response variables based on the proportions of component for this mixture. In our research we will compare the scheffʼe model with the kronecker model for the mixture experiments, especially when the experimental area is restricted.
Because of the experience of the mixture of high correlation problem and the problem of multicollinearity between the explanatory variables, which has an effect on the calculation of the Fisher information matrix of the regression model.
to estimate the parameters of the mixture model, we used the (generalized inverse ) And the Stepwise Regression procedure
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The determination of captopril (CAP) using a new continuous flow injection analysis (CFIA) method was given in this work CAP in its pure state and some of its pharmaceutical preparations. The technique can be described as simple, fast, sensitive, easy to operate, and low-cost. The CAP reacted with ammonium ceric(IV) sulfate (ACS)2(NH4 )2SO4Ce(SO4)2. 3 H2O in an acidic medium and the reaction led to the formation of a white, slightly yellowish precipitate. The formed precipitate was studied using Ayah 6S×1-ST-2D Solar cell-CFI Analyzer, a through the reflection of accident light on the surfaces of the precipitate particles at (0-1800), expressed as the response
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A new simple sensitive and selective spectrophotometric method has been developed for the analysis of vanadium(V) in three randomly chosen samples from river water at different locations by continuous flow injection analysis. The method based on the oxidation of pyrogallol by vanadium(V) in acidic solution to form color species and the same species was determined using homemade Ayah 6SX1-T-2D solar cell analyser . Chemical and physical parameters were investigated using the high intensity of snow white light emitted diode as a source. The linear dynamic range for the instrument response versus vanadium(V) concentration was 1-200 mg.L-1 with correlation coefficient r = 0.9920. The limit of detection (S/N=3) was 70 ng/ sample from the step
... Show MoreNew mode for the on-line determination of oxonium ion in different strong acids using CFIA via the use of homemade linear array Ayah 5Sx4-ST- 5D solar CFI analyser
A new simple sensitive and selective spectrophotometric method has been
developed for the analysis of vanadium(V) in three randomly chosen samples from
river water at different locations by continuous flow injection analysis. The method
based on the oxidation of pyrogallol by vanadium(V) in acidic solution to form color
species and the same species was determined using homemade Ayah 6SX1-T-2D
solar cell analyser . Chemical and physical parameters were investigated using the
high intensity of snow white light emitted diode as a source. The linear dynamic
range for the instrument response versus vanadium(V) concentration was 1-200
mg.L-1 with correlation coefficient r = 0.9920. The limit of detection (S/N=3) was 70<