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Study the effect of the different doses from the laser on Staphylococcus aureus Bacteria growth in vitro

Background: Laser is a novel physical therapy technique used to treat various conditions, including wound healing, inhibition of bacterial growth, and postoperative wounds. High-power pulsed alexandrite laser therapy is one of the most prevalent forms of laser therapy, which is a noninvasive method for treating various pathological conditions, thereby enhancing functional capacities and quality of life. It is a modern medical and physiotherapeutic technology. Generally, the Alexandrite laser emits infrared light with a wavelength of 755 nm, allowing it to propagate and penetrate tissues. Objective: This study focused on the application of a high-power pulsed alexandrite laser in vitro to evaluate the effect of a pulsed alexandrite laser on antibiotic-resistant bacteria utilizing varying exposure times, pulse durations, and laser fluencies to determine which dose is more effective on S. aureus bacteria. Method: The laser used in this study was the alexandrite laser which was considered a pulsed laser and had the following parameters: The wavelength was 755 nm, the beam diameter was (14 mm), the exposure times varied (30, 60, 90) seconds, the laser fluency (5, 10, 15 and 20 J.Cm-2). The study was carried out after the bacteria were diagnosed as being antibioticresistant. They were exposed to different doses of Alexandrite laser. Three samples of bacteria were exposed to laser beams for 30 seconds with a 5ms pulse duration and with a laser fluency of 5J/cm2, and this process was repeated with laser fluencies of 10, 15, and 20. This procedure was repeated using exposure times of 60sec and 90sec. As well as, this process was repeated by exposure with 30 sec, 60 sec and 90 sec exposure times, 10ms and 20ms pulse durations and with different laser fluencies 5, 10, 15 and 20J/cm2, separately. Results: A significant reduction (p = <0.0001) in the mean values of the colony was observed with the increase of laser fluency doses compared with control at the same pulse duration. A significant reduction (p = <0.0001) in the mean count of the colonies was observed in the comparison between two laser fluences at the same pulse duration. In conclusion, the exposure times, pulse durations and laser fluencies of pulsed alexandrite laser showed an effect on the mean count of the colonies of S aureus bacteria and determined the effective dose. Keywords: laser, Staphylococcus aureus, Bacteria growth

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Publication Date
Mon Jul 24 2023
Journal Name
Pollution Research
THERMODYNAMIC AND KINETIC STUDY OF ADSORPTION OF THE AZO DYE FROM AQUEOUS SOLUTION ON THE SURFACE OF IRAQI FLINT

EM International

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Publication Date
Thu Nov 28 2024
Journal Name
Journal Of Baghdad College Of Dentistry
Effects of three different types of intracoronal bleaching agents on shear bond strength of stainless steel and sapphire brackets bonded to endodontically treated teeth (An in vitro study)

Background: evaluate the effects of three different intracoronal bleaching agents on the shear bond strengths (SBS) and failure site of stainless steel and monocrystalline (sapphire) orthodontic brackets bonded to endodontically treated teeth using light cured orthodontic adhesive in vitro. Materials and methods: Eighty extracted sound human upper first premolars were selected, endondontically treated and randomly divided equally (according to the type of the brackets used) into two main groups (n = 40 per group). Each main group were subdivided (according to the bleaching agent used) into four subgroups 10 teeth each; as following : control (un bleached) group, hydrogen peroxide group (Hp) 35%, carbamide peroxide group (CP) 37% group and s

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Publication Date
Wed May 17 2017
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Detection for The Production of Siderophore in Burkholderia cepacia Isolated From the Rhizosphers of Some Plants and studing the Effect of the Bacterial Filtrate and Extract on Some Pathogenic Fungi

bstract         This study was performed to isolate and identify the Burkholderia cepacia from rhizosphers of different plants and study their ability to produce Siderophore, the study was also aimed to assess the antifungal activity of the bacterial filtrate and extracts of the isolates against some pathogenic fungi.     The isolate which showed higher inhibitory effects was selected for extraction of some active metabolites produced by it, and evaluate the activity in vitro, via inhibition of the growth of the fungi using different concentrations of extract (50, 100, 200 )µL/ml , The results showed that out of  (250) samples of soil , (8) isolates (3.2%) gave positive

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Publication Date
Sun Sep 04 2011
Journal Name
Baghdad Science Journal
Study the Effect of Ocimum basilscm oil in the Sensory properties of Laboratory Biscuit

The study aims at showing the effect of basil oil on the sensory properties of the laboratory biscuits. the results show the sensory valuation before and after the storage. the (A4) equation exceeds 15% of T exchange (3 , 4 , 5 g. basil 19 , 55 g . fats )in most of the studied sensuous qualities. Then, the (A5) equation of 20% exchange percentage (4.60g. basil oil + 18.40g. fats). Then, the (A3) equation of 10% exchange percentage (2.30g. basil oil + 20.70g. fats). Then the (A2) equation of 5% exchange percentage (1.5g. basil oil + 21.85g. fats). Finally, the control equation (A1) received the lower value of sensuous evaluation and general acceptance. Abstract differences also appeared at denotation level 0.05 between the (A2)

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Publication Date
Tue Dec 13 2005
Journal Name
Iraqi Journal Of Laser
Stimulation of Phagocytic Activity of Human Polymorphonuclear Leukocytes in Vitro Using 10 mW He-Ne Laser.

Stimulative effect of 10 mW He-Ne laser on the phagocytic activity of human polymorphonuclear leukocytes( PMNs) has been studied in vitro. Normal polymorphonuclear leucocytes were isolated from the human peripheral blood. A mixture of 0.25 ml of Hanks solution, 0.25 ml of serum, 0.25 ml of Candida albicans suspension and 0.25 ml of PMNs suspension was prepared. The samples of mixture of PMNs and Candida were subdivided in 1 ml ependrof tubes and irradiated to He-Ne laser for 1, 3, 5, 10 and 20 min. The diameter of the irradiated area was 0.8 cm. For calculation of Phagocytic index before and after irradiation, the samples were incubated (37°C) at 5, 15, 30, 60 min. The slides of samples were prepared and stained using Giemsa stain. The

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Publication Date
Sun Jun 02 2013
Journal Name
Baghdad Science Journal
Comparison of The Effect of Aqueous Extracts of two Plants, Origanum Vulgare L. and Fenugreek Seeds with Anticancer Drug Cis-Platin on the Growth of Cancer Cell Lines

This study involved the effect of the aqueous extracts of two plants, Origanum vulgare L.(1), Trigonella Foenum Graecum L. (Fenugreek) seeds(2) on the growth of cancer cell lines. Rhabdomyo sarcomas (RD) of human cell line and female intestine cells of Albino mice (L20B) in vitro System. These extracts were compared with the known anticancer drug Cis-platinum(Cis-Pt) as a positive control. The phytochemical tests were used for screening the active compounds in plants. The inhibition activity assay was used as a parameter of the cytotoxic effect of these extracts. Cancer cell lines were treated with four concentrations of Cis-platin, 31.25, 62.5, 125 and 250 ?g/ml for 72 hour exposure time. The same concentrations were used for the other ext

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Publication Date
Wed Dec 30 2015
Journal Name
College Of Islamic Sciences
The phenomenon of building the act of the actor and the effect in the Quranic readings                          (Structural and semantic study)

After this systematic presentation of the phenomenon of rotation of formulas, ie the construction of the verb and the verb, and the narrated narratives therein differed among the readers, it became clear to us how the difference was clear between reading and the other, and how readers have varied in their readings of the verb, As we have noted through our modest research that the phenomenon of building the verb for the actor and the effect have included the act of both past and present tense, and not limited to a specific time, and this difference in reading was not limited to a particular environment, but beyond To more than one environment This is evidenced by the readings of various readers from the environment of Kufa, Basra, Mecca,

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Publication Date
Wed Jan 15 2020
Journal Name
Iraqi Journal Of Laser
Electronically Implementation and Detection of Pulse Laser from Continuous Laser Diode

This research aims to design a high-speed laser diode driver and photodetector, the result is the
design of the high-speed laser diode driver with a short pulse of 10 ns at 30 KHz frequency and the
delivered maximum pulse voltage is 5.5 mV. Also, its optical output power of the laser diode driver is
about 2.529 mW for the centroied wavelength 1546.7 nm with FWHM of 286 pm and (1270-1610) nm.
The design of the circuit based on bipolar transistor where the input pulse signal is simply generated by
an arduino kit with 15 kHz frequency and then compensated to trigger to small signal amplifier which
was is simply NPN C3355 transistor and the output is a current driver to the laser diode. OptiSystem
software and Electronic

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Publication Date
Sat Feb 26 2022
Journal Name
Iraqi Journal Of Science
Effects of Psidium Guajava Leaves Extract on the Viability of Echinococcus Granulosus Protoscolices in Vitro and in Vivo

     Echinococcosis is a common parasitic disease in humans and ruminants; it is considered as a health and economic problem in most parts of the world. The present study aims to evaluate the in vitro and in vivo scolicidal effects of Psidium guajava extracts on the protoscolices from hydatid cysts of Echinococcus granulosus. Protoscolices were aseptically aspirated from the livers of naturally infected sheep. Various concentrations of the extract were used for 6-48hrs. Eosin staining (0.1%) was used to determine the viability of protoscolices. The findings showed that the extract at the concentrations of 100 mg/ml killed 100% protoscolices after 48 hrs. However, the concentrations

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Publication Date
Fri May 05 2023
Journal Name
International Journal Of Professional Business Review
The Effect of Emotional Intelligence on Manager Performance: a Field Study in the General Authority for Irrigation and Reclamation Projects in Baghdad

Purpose: the purpose of this study is to investigate how managers working for the General Authority for Irrigation and Reclamation Projects react to the impact of Emotional Intelligence (EI) on their performance.   Theoretical framework: The current study includes an intellectual framework on two variables, namely EI and Manager Performance (MP), because it is essential to investigate the relationship between these two variables and the impact of EI on MP.   Design/methodology/approach: The research problem is that a manager's capacity to make wise decisions about their work or interactions with subordinates is diminished when they have inadequate EI. The questionnaire is used as a tool for gathering data for the study, and the st

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