Chemical pesticides have an impact on other living organisms in addition to their intended target organisms. Any chemical pesticide is therefore made safe for use by examining its biological characteristics and side effects. The present study was aimed at determining the resistance efficiency of six bacterial isolates obtained from malathion-contaminated soils. Bacteria were isolated from soil samples collected in Adhamiya, Baghdad, Iraq. Biochemical tests and VITEK 2 compact equipment were used to identify the bacterial isolates. Primary and secondary screening tests were conducted on the bacterial isolates for resistance against malathion pesticides. The optimal bacterial growth conditions were determined in malathion-contaminated media. The results demonstrated that the bacterial isolates 1, 3, 4, and 8 grew best on malathion-contaminated (100 mgL-1) mineral salt medium (MSM). Isolates 1 and 2 had a MIC of 500 mgL-1, where they continued to grow until the seventh day of incubation. Pseudomonas aeruginosa, Pseudomonas putida, Aeromonas hydrophilia, and Escherichia coli were the identified bacterial isolates. These isolates showed optimal bacterial growth in the test conditions (temperature, incubation period, and pH), indicating their efficiency and ability to degrade malathion. The highest average growth of P. aeruginosa at 35 °C was 70.08 mm, while that of P. putida was 40.10 mm during the 7-day incubation period. Also, the highest values of average growth were observed in the same bacteria at pH 7, with a value of 26.98 mm. The findings of this study reveal that Pseudomonas aeruginosa and Pseudomonas putida were the best bacterial isolates for biodegrading malathion.
م.د. فاطمة حميد ،أ.م.د وفاء صباح محمد الخفاجي, International Journal of Psychosocial Rehabilitation,, 2020 - Cited by 1
The research aims at identifying the level of family violence directed against the kindergarten child, as well as to identify the significance of the difference in family violence directed against the kindergarten child according to the gender variable
(male-female). It aims also to identify the significance of the difference in family violence directed against the kindergarten child according to the Stage variable (kindergarten-pre-primary). The researcher employed the descriptive correlative approach to conduct the study. The research sample was chosen in a simple random way, as the number of the participants was (130) boys and girls, with (70) males and (60) females, distributed according to the stage with (66) participants in
... Show MoreDuring the last few yt:ars, au increase :n ut ilization of contact ienses (both cosmetic and correction of refractive errors lenses) was noticed among yotmg females, with an increase in eye infections. The present i nvocation was carried out to demonstrate the percentage of bacter ial contamination of both the preservation fluid and lens oonlainers. Fifty two contact lenses users among female students and em I ployees of
college of Education-Ibn Al- Haitham were surveyed. Data were
obtained on methodof usage and results of microbial examination. The &nb
... Show MoreThe molluscicidal effects of herbicide 2, 4-D were studied against tow species of freshwater snail Bulinus truncatus and Melanopsis nodosa by short term experiments. Calculated values of lethal concentrations (LC50 and LC100) were maid to two spices for different period of time 24hr, 48hr, 72hr and 96hr. The study had showed that the herbicide 2, 4-D was toxic against the tow species. The toxicity of the herbicide was low or unknown in low concentrations in the first period of exposure 24and 48hr to two spices but it increase gradually with increase period of exposure. The spice of B.truncatus was more tolerant than the M.nodosa. All the individual of M nodosa was death while in B.truncatus the complete death was not appear until finish the
... Show MoreThe main aim of this study is to investigate the ability of four local entomopathogenic isolates Beauveria bassiana (Bals.) and Metarhizium anisopliae (Met.) to control the mosquito larvae in the lab. The results revealed that the isolate (MARD48) B .bassiana reduced the survival rate of the mosquito larvae to (80%) followed by the isolate M. anisopliae (MARD10) to (90%) in the first two days of treatment, and 60 and 66% respectively in the third day. The results also showed that the isolate B. bassiana (MARD48) killed 50% of the population (LC50) with the concentration 1×104 conidia/ml compared to 1×107 conidia/ml for the isolates B. bassiana (MARD14) and M. anisopliae (MARD10), and 1×108 conidia/ml for the isolate B .bassiana (MARD76).
... Show MoreThe main aim of this study was to molecular identification and determine the antagonistic impact of rhizosphere Trichoderma spp. against some phytopathogenic fungi, including (Magnaporthe grisea) pyricularia oryzae, Rhizoctonia solani and Macrophomina phasolina. Four Trichoderma isolates were isolated from rhizosphere soils of the different host plants in different locations of Egyptian governorates. The morphological characterization of isolated Trichoderma as well as using of (ITS1-5.8S-ITS2) ribosomal gene sequence acquisition and data analyses. By comparing the results of DNA sequences of ITS region, the fungi represented one isolate were positively identified as T. asperellum (1 isolate T1) and one as T. longibrachiatum (1 isolate T2)
... Show MoreOver the past few decades, the health benefits are under threat as many commonly used antibiotics have become less and less effective against certain illnesses not only because many of them produce toxic reactions but also due to the emergence of drug-resistant bacteria. The clinical use of a combination of antibiotic therapy for Pseudomonas aeruginosa infections is probably more effective than monotherapy. The present study aims to estimate the antibacterial and antibiofilm activity of Conocarpus erectus leaves extracts against multi-drug resistant P. aeruginosa isolated from different hospitals in Baghdad city. One hundred fifty different clinical specimens were collected from patients from September 2021 to January 2022. All samples were
... Show MoreA sample of 168 tonsillitic subjects (93 males and 75 females), livin g in Baghdad was evaluated microbiologically. The patients had an age range of 3-35 years. Four bacterial species were isolated and identified. They were P-haemolyt ic Streptococcus ,(39.3%) Staphylococcus
... Show MoreFlame thermal spray technique has been used to produce a cermets composite coating based on powders of ceramic oxides (
AhOJ) reinforced by mineral powders as bonding material ( Ni - AI ) at different rates on Inconel substrates , after preparing it by grit blasting.
After completing the best parameters of coating such as distance of spraying , surface roughness and angle of spraying , the tests of porosity , hardness , microstructure and thermal cycling have been made.
The experimental results show that all properties of coating were effected after heat treatment. The best value of heat treatmen t is at (I 000°C) for (l.Shr) in which the &
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