doi:10.1111/plb.12096

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Publication Date

Mon Sep 09 2013

Journal Name

Plant Biology

Volume

16

DOI

10.1111/plb.12096

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Comparing simple root phenotyping methods on a core set of rice genotypes

R.

Z

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Abstract<p>Interest in belowground plant growth is increasing, especially in relation to arguments that shallow‐rooted cultivars are efficient at exploiting soil phosphorus while deep‐rooted ones will access water at depth. However, methods for assessing roots in large numbers of plants are diverse and direct comparisons of methods are rare. Three methods for measuring root growth traits were evaluated for utility in discriminating rice cultivars: soil‐filled rhizotrons, hydroponics and soil‐filled pots whose bottom was sealed with a non‐woven fabric (a potential method for assessing root penetration ability). A set of 38 rice genotypes including the Oryza<styled-content style="fixed-case">SNP</styled-content> set of 20 cultivars, additional parents of mapping populations and products of marker‐assisted selection for root <styled-content style="fixed-case">QTL</styled-content>s were assessed. A novel method of image analysis for assessing rooting angles from rhizotron photographs was employed. The non‐woven fabric was the easiest yet least discriminatory method, while the rhizotron was highly discriminatory and allowed the most traits to be measured but required more than three times the labour of the other methods. The hydroponics was both easy and discriminatory, allowed temporal measurements, but is most likely to suffer from artefacts. Image analysis of rhizotrons compared favourably to manual methods for discriminating between cultivars. Previous observations that cultivars from the <italic>indica</italic> subpopulation have shallower rooting angles than <italic>aus</italic> or <italic>japonica</italic> cultivars were confirmed in the rhizotrons, and <italic>indica</italic> and <italic>temperate japonicas</italic> had lower maximum root lengths in rhizotrons and hydroponics. It is concluded that rhizotrons are the preferred method for root screening, particularly since root angles can be assessed.</p>

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Publication Date

Fri Jan 13 2023

Journal Name

Journal Of The Faculty Of Medicine Baghdad

Detection of Chronic Obstructive Pulmonary Disease among Smokers Aged ≥40 years Attending Primary Health Care Centers in Baghdad-AL-Karkh and AL-Resafa: الكشف عن الالكشف عن مرض الانسداد الرئوي المزمن بين المدخنين الذين تزيد أعمارهم عن 40 عامًا يراجعون مراكز الرعاية الصحية الأولية في بغداد - الكرخ و الرصافة

Isra A.

Nada Abdul Wahhab

Faris

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Background: Chronic obstructive pulmonary disease causes permanent morbidity, premature mortality and great burden to the healthcare system. Smoking is it's most common risk factor and Spirometry is for diagnosing COPD and monitoring its progression.

Objectives: Early detection of chronic obstructive pulmonary disease in symptomatic smokers’ ≥ 40years by spirometry.

Methods: A cross sectional study on all symptomatic smokers aged ≥ 40 years attending ten PHCCs in Baghdad Alkarkh and Alrisafa. Those whose FEV1/FVC was <70% on spirometry; after giving bronchodilator, were considered COPD +ve.

Results: Overall, airway obstruction was seen in

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