Comparing simple root phenotyping methods on a core set of rice genotypes

R. Shrestha; Z Al-Shugeairy

doi:10.1111/plb.12096

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Publication Date

Mon Sep 09 2013

Journal Name

Plant Biology

Volume

16

DOI

10.1111/plb.12096

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Comparing simple root phenotyping methods on a core set of rice genotypes

R. Shrestha

Z Al-Shugeairy

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AbstractInterest in belowground plant growth is increasing, especially in relation to arguments that shallow‐rooted cultivars are efficient at exploiting soil phosphorus while deep‐rooted ones will access water at depth. However, methods for assessing roots in large numbers of plants are diverse and direct comparisons of methods are rare. Three methods for measuring root growth traits were evaluated for utility in discriminating rice cultivars: soil‐filled rhizotrons, hydroponics and soil‐filled pots whose bottom was sealed with a non‐woven fabric (a potential method for assessing root penetration ability). A set of 38 rice genotypes including the Oryza<styled-content style="fixed-case">SNP</styled-content> set of 20 cultivars, additional parents of mapping populations and products of marker‐assisted selection for root <styled-content style="fixed-case">QTL</styled-content>s were assessed. A novel method of image analysis for assessing rooting angles from rhizotron photographs was employed. The non‐woven fabric was the easiest yet least discriminatory method, while the rhizotron was highly discriminatory and allowed the most traits to be measured but required more than three times the labour of the other methods. The hydroponics was both easy and discriminatory, allowed temporal measurements, but is most likely to suffer from artefacts. Image analysis of rhizotrons compared favourably to manual methods for discriminating between cultivars. Previous observations that cultivars from the <italic>indica</italic> subpopulation have shallower rooting angles than <italic>aus</italic> or <italic>japonica</italic> cultivars were confirmed in the rhizotrons, and <italic>indica</italic> and <italic>temperate japonicas</italic> had lower maximum root lengths in rhizotrons and hydroponics. It is concluded that rhizotrons are the preferred method for root screening, particularly since root angles can be assessed.

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Publication Date

Sun Jan 01 2012

Journal Name

Evidence-based Complementary And Alternative Medicine

Gelam Honey Inhibits the Production of Proinflammatory, Mediators NO,<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M1"><mml:mrow><mml:msub><mml:mrow><mml:mtext>PGE</mml:mtext></mml:mrow><mml:mtext>2</mml:mtext></mml:msub></mml:mrow></mml:math>, TNF-α, and IL-6 in Carrageenan-Induced Acute Paw Edema in Rats

Saba Zuhair

Kamaruddin

Suzana

Yasmin Anum

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Natural honey is well known for its therapeutic value and has been used in traditional medicine of different cultures throughout the world. The aim of this study was to investigate the anti-inflammatory effect of Malaysian Gelam honey in inflammation-induced rats. Paw edema was induced by a subplantar injection of 1% carrageenan into the rat right hind paw. Rats were treated with the nonsteroidal anti-inflammatory drug (NSAID) Indomethacin (10 mg/kg, p.o.) or Gelam honey at different doses (1 or 2 g/kg, p.o.). The increase in footpad thickness was considered to be edema, which was measured using a dial caliper. Plasma and paw tissue were collected to analyze the production of inflammatory mediators, such as NO, PGE₂

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