Background: Using dual-energy X-ray absorptiometry, body fat mass has been determined. The assessment of body fat mass was conducted utilizing dual-energy X-ray absorptiometry analysis of the pelvis and vertebral column. While it is acknowledged that osteoporosis can impact both body fat mass and bone mineral density, the particulars of this relationship currently remain uncertain. Objective: The aim of the present investigation is to assess gender differences in the effects of osteoporosis on the body fat mass of the upper and lower extremities. Method: 170 individuals participated (85 males and 85 females) in this study. Patients who presented with bone discomfort consisted of 40 males and 40 females. In addition, 90 apparently healthy volunteers, consisting of 45 males and 45 females, were studied and considered to constitute the control group. Dual-energy X-ray absorptiometry was utilized to determine the bone mineral density and body fat mass of every participant for all body parts. Results: Statistically significant disparities in body fat mass were observed between males and females, as well as between the control group and patients diagnosed with osteoporosis. Conclusions: Our results indicate that the patients with osteoporosis showed an increase in body fat mass (for both sexes). Other results obtained from this research revealed that females were more frequently suffering from osteoporosis than males.