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Reverse Phase High Performance Liquid Chromatography (RP-HPLC) was coupled with
ultraviolet absorption sepectoscopy (UV) for separation and identification of Naphthalene,
Acenaphthylene, Pyrene, Benz{a} anthracene and 1,3,2,4-Dibenzanthracene.
RP-HPLC was performed on an ODS-C18 column (150×4.6 mm I.D) using acetonitrile–buffer
phosphate as mobile phase. UV absorption spectra of the elutes was detected in 254 nm, and
studying the chromatographic variables include organic modifier ratio, PH, column temperature and
concentration of buffer to maximize resolution and minimize separation time. the results showed
that using mobile phase( 80:20) v/v acetonitrile:0.01M phosphate buffer solution at PH 6 with flow
rate 1ml/min and column te
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