Investigations made and soil samples brought from 14 sites in different areas, including Abu Ghraib and Al Anbar. Tests and measurements made in the Microbiology Laboratory at the College of Agriculture, University of Anbar. Department of Soil Sciences and water Resources, in order to isolate bio-fertilizers and test isolates fixing nitrogen in atmosphere and solvents for phosphorous compounds efficiency. The experiment included isolating and diagnosis of bacteria from rhizosphere soils of different plants that were brought from different agricultural areas, 74 isolates obtained by soils alleviation, and then the bio-chemical morphological and microscopic characteristics of these soils studied. The results showed that the most abundant and frequent isolates were Azotobacter 15 isolates, Bacillus 15 isolates, Pseudomonas 11 isolates, and Azosperillium 10 isolates and Actinomycetes 10 isolates. Nitrifying bacteria of both geneas Nitrosomonas 7 isolates and Nitrobacter 6 isolates were existed while the numbers of bacteria Azotobacter and Bacillus which fixing Nitrogen in free form and solvent phosphate compounds in the soil are exsist in the soil of the rhizosphere of different plants used in research in agricultural fields. The highest biodiversity rate of isolates was found in Fallujah Nuaimia field in 12 hectares, which pepper planted, with an average of 11 isolates and the lowest rate was 2 isolates in the soil of Heet Basayer field were palms planted in an area of 5 hectares. Chemical and physical Features as well as some heavy elements were estimated elements. Also the concentrations of each site that the samples were taken were estimated. Obtained in the laboratory of the College of Life Sciences at the University of Baghdad. The most important results were the estimated elements were in normal and allowed average except a little increase in zinc concentration 90 mg kg 1 in Sofia field, 7 hectares, were Jet planted, and the concentration of lead was 24.3 mg kg - 1 in Abu Ghraib Nibras field of 80 hectares, which planted with wheat.