An epidemiological study in Al-Mahmmodiya (50 km south Baghdad) to investigate a possible vector of Leishmaniasis were applied .This region is considered as a foci of Leishmaniasisaccording to the health association statistics. CDC light traps were used to collect the insects nightly. Insects were collected by Indoor application as human dwellings and animal shelters and Outdoor application as rodent's barrows and field trees. Sand flies were transported to the laboratory, isolated and identified according to the identification keys in Tropical Biological Researches Unit at the Collage of Science / University of Baghdad. Must of the collected sand flies were belonging to three species PhlebotomuspapatasiScopoli(45%), PhlebotomusalexandriSinton (35%) and SergentomiyabaghdadisAdler & Theodor (20%). About 896 feeding females of sandflies were dissected; only 32 ones were infected with the parasites identified as Leishmaniaspp. five samples were cultured in parasite media to insure the type of parasite. Only the specie of Phlebotomuspapatasi was containing parasites inside. The culture media was contaminated, so the attempts to get pure culture to discover the possibility of transport another type of parasite are continue. The aim of the study is to investigate a possible vector of Leishmaniasis in Al-Mahmmodiya region.