Using PCR and Gel Electrophoresis Techniques to Molecular Confirm and Detection for Flowering Gene Presence in Maize Hybrids

Al-Baidhani H.H. Al-Baidhani H.H

doi:10.1088/1755-1315/1158/6/062037

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Publication Date

Sat Apr 01 2023

Journal Name

Iop Conference Series: Earth And Environmental Science

Volume

1158

DOI

10.1088/1755-1315/1158/6/062037

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Using PCR and Gel Electrophoresis Techniques to Molecular Confirm and Detection for Flowering Gene Presence in Maize Hybrids

Al-Baidhani H.H. Al-Baidhani H.H

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Abstract<p>The laboratory experiment was conducted in the laboratories of the Musayyib Bridge Company for Molecular Analyzes in the year 2021-2022 to study the molecular analysis of the inbreed lines and their hybrids F1 to estimate the genetic variation at the level of DNA shown by the selected pure inbreed lines and the resulting hybrids F1 of the flowering gene. Five pure inbreed lines of maize were selected (ZA17WR) Late, ZM74, Late, ZM19, Early ZM49WZ (Zi17WZ, Late, ZM49W3E) and their resulting hybrids, according to the study objective, from fifteen different inbreed lines with flowering time. The five inbreed lines were planted for four seasons (spring and fall 2019) and (spring and fall 2020) in the spring season 2019 the inbreed lines were crossed and flowering time were recorded and in the fall season 2019 they were crossed according to the study objective (late × late), (late × early) and (early) × late) and (early × early) in the third season, The results indicated that the two initiators used with the target flowering gene are highly efficient in diagnosing genetic variations and genetic divergence between the selected inbreed lines and their resulting hybrids F1 according to the different flowering time using PCR Poly Chain Reaction and Gel electrophoresis techniques. ) and the fourth hybrid (early×early) was superior in most of its field traits. It was found that inbreed line No. 15 (ZA17WR) did not show any bands in the interaction of the PCR and the flowering gene, and this is evidence that this inbreed line is counted as being optimal for the target gene and therefore genes or genetic sites may influence the early or delayed flowering time trait Therefore, it needs future studies. The aim of the current study is to know the genetic structures that contain the flowering gene and according to the planting date, whether fall or spring, to take advantage of those results in choosing and determining the appropriate and most appropriate method in the maize crop breeding programs to obtain promising genetic structures in terms of flowering time, whether was early or late. Additionally using two techniques to gather leads to increase the usefulness of these two techniques as the number of examined hybrids and inbred lines continues to increase rapidly.</p>

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Tue Dec 28 2021

Journal Name

2021 2nd Information Technology To Enhance E-learning And Other Application (it-ela)

Pedestrian and Objects Detection by Using Learning Complexity-Aware Cascades

Mohammed F. Alrifaie

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Publication Date

Tue Aug 05 2014

Journal Name

International Journal Of Pharmacy And Pharmaceutical Sciences

FORMULATION AND EVALUATION OF FLOATING ORAL IN-SITU GEL OF METRONIDAZOLE

Gastro-retention

in situ gel

sodium alginate

metronidazole

Lena

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Publication Date

Tue Jul 02 2024

Journal Name

Al-rafidain Journal Of Medical Sciences ( Issn 2789-3219 )

Formulation and Evaluation of Bilastine Thermosensitive Mucoadhesive Ophthalmic in situ Gel

Alaa Ghazi

Lena Murad

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Background: Bilastine is a non-sedating, second-generation antihistamine used to treat urticaria and allergic conjunctivitis. Objective: to formulate and test bilastine as a mucoadhesive ophthalmic in situ gel in order to extend its presence at site for longer time and help treat conjunctivitis and allergic rhinitis. Methods: We prepared formulations using different concentrations of poloxamers (Poloxamer 407 (P407) and Poloxamer 188 (P188)) in combination with hydroxypropyl methyl cellulose (HPMC). The prepared formulas were evaluated for their physicochemical properties, sol-gel transition temperature, viscosity, mucoadhesive strength, drug release, and kinetic modeling. Results: The prepared in situ gels were clear and transparen

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Publication Date

Wed Mar 29 2017

Journal Name

Iraqi Journal Of Pharmaceutical Sciences ( P-issn 1683 - 3597 E-issn 2521 - 3512)

Preparation and Evaluation of Chloramphenicol as Thermosensitive Ocular in- situ Gel

Ali

Shaimaa

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The purpose of this study was to develop poloxamer-based in-situ gel of chloramphenicol aiming to increase bioavailability and prolong corneal contact time, controlling drug release, and enhancing ocular bioavailability. The in-situ gel was prepared using different concentrations of poloxamer 407 combined with hydroxypropyl methyl cellulose (HPMC) or carbapol 940 to achieve gelation temperature about physiological temperature and improve rheological behavior and gelling properties of poloxamer gel. The prepared formulations were evaluated for their appearance, pH, and sol-gel transition temperature. The formulations F₂, F₃, and F₅ have a gelation temperature within the accepted range 35-37⁰C an

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(6)

Publication Date

Sat Dec 05 2015

Journal Name

PrzeglĄd Elektrotechniczny

Power Amplifier Frequency Controller Using feedback control techniques for Bio-implanted Devices

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Thu Jun 25 2020

Journal Name

Scientific Reports

Lysophosphatidylcholine acyltransferase 2 (LPCAT2) co-localises with TLR4 and regulates macrophage inflammatory gene expression in response to LPS

LPCAT2

LPS

LPCAT1

TLR4

TLR2

siRNA

shRNA

macrophage

cytokine

gene expression

W.

H.

M.

A. J.

S. K.

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Despite extensive investigations, an effective treatment for sepsis remains elusive and a better understanding of the inflammatory response to infection is required to identify potential new targets for therapy. In this study we have used RNAi technology to show, for the first time, that the inducible lysophosphatidylcholine acyltransferase 2 (LPCAT2) plays a key role in macrophage inflammatory gene expression in response to stimulation with bacterial ligands. Using siRNA- or shRNA-mediated knockdown, we demonstrate that, in contrast to the constitutive LPCAT1, LPCAT2 is required for macrophage cytokine gene expression and release in response to TLR4 and TLR2 ligand stimulation but not for TLR-independent stimuli. In addition, cells transfe

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Publication Date

Wed Aug 01 2018

Journal Name

Ecotoxicology And Environmental Safety

Biochemical and molecular alterations in freshwater mollusks as biomarkers for petroleum product, domestic heating oil

Ali Abdulhamza

Adel M.

Ayad M.J.

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Publication Date

Fri Jan 22 2021

Journal Name

International Journal Of Agricultural & Statistical Sciences

EFFECT OF GROWING MEDIA, NUTRIENT SOLUTION AND CONTROL OF FLOWERING ON THE PRODUCTIVITY OF THE CUCUMBER CULTIVATED BY THE SOILLESS CULTURE.

Abdulraheem Assi

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Publication Date

Sun Apr 17 2016

Journal Name

Al-academy

The optical text in the cinema film Between the presence and absence Play for free mark: حسام الدين محمد عبد المنعم

HussamulDeen

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The light-based life in the universe, including the human derived concepts and meanings of the fear of darkness and evil, comfort and goodness of light, became constitute bilateral haunted man to this day in various concepts of life.Therefore reflect the light form artistic aesthetic in visual arts such arts Fine Photography and the other until the emergence of art cinema, as the use of lighting in cinema has produced high-energy in the composition of its values expressive and symbolic, where it became dark and light are the space visually moving the vehicles media kit for many within the work structure artwork. The research is divided into five chapters, the first chapter (the methodological framework) that included an introduction the

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Publication Date

Thu May 13 2021

Journal Name

International Journal Of Development In Social Science And Humanities

The Impact of Teachers using Storytelling Techniques through Virtual Instruction to Increase English Speaking Ability

Bushra

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DBN Rashid, INTERNATIONAL JOURNAL OF DEVELOPMENT IN SOCIAL SCIENCE AND HUMANITIES, 2021

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