Lactiplantibacillus plantarum, one of lactic acid bacteria (LAB), is found in various foods, including dairy products, meat, and vegetables, and most of these bacteria offer beneficial effects to humans and animals as potential probiotics with broad-spectrum antimicrobial activities. The aim of this study was evaluating the antibacterial efficacy of L. plantarum against some foodborne bacteria isolated from dairy products. This research involved 34 dairy products, including local and imported milk, cheese, and yogurt sold locally in Baghdad province, Iraq, during May 2022. For the isolation of L. plantarum, a special medium called MRS (de Man Rogosa and Sharpe) was applied. Colonies were purified and identified by routine bacteriological methods, Vitek2 system, and confirmed by the polymerase chain reaction (PCR) targeting the 16S rRNA gene followed by the amplicon sequencing. Other aerobic bacteria contaminating dairy products were also isolated onto sterile selective media specific for each microorganism, and the isolates were identified by routine diagnostics tests followed by verification with Vitek2 system. Then, the culture supernatant of L. plantarum was tested for its antagonistic activity toward foodborne bacteria by the use of agar well diffusion assay. The findings showed the isolation of 2 L. plantarum, 3 Pseudomonas aeruginosa, 4 Escherichia coli, one isolate of Bacillus subtilis, and another Staphylococcus hominis. The filtered supernatant of L. plantarum was significantly efficient in inhibiting the growth of the above bacteria. Each of E. coli and B. subtilis revealed zones of inhibition of 36 and 38 mm in diameter, respectively, while P. aeruginosa and S. hominis had inhibition zones diameters of 27 and 29 mm, respectively. This suggests that the L. plantarum supernatant possesses a broad-spectrum activity against foodborne bacteria. To conclude, locally made dairy products can hold different contaminating bacteria, which can be eliminated by using probiotics, such as L. plantarum, to avoid foodborne diseases onset.
Forty eight isolates (41.02%) were obtained from 117 wound and burn samples. The isolates that showed high resistance for both antibiotic was two only that represent 4,1% from all isolates. The result of PCR product electrophoresis was referred that the gene is VIM gene. Lactose and raffinose showed double increasing in diameter of inhibition zone of imipenem with 1% that mean showed highest susceptibility that decreased with the concentration increasing, the same result were with meropenem. But no effect were detected on meropenem inhibition zone diameter. Mannose have no effect on the resistance in 1%, 3% and 7%. Results showed that only three case that increase the expression of gene, they were lactose at 1% concentration that increased
... Show MoreThis work includes synthesis of sugar tetrazole derivative, D-ribose reacted with acetone in the presence of sulfuric acid H2SO4 to give 2, 3-O-isopropylidene-D-ribose (1). The Aldol condensation of (1) with formaldehyde in methanolic K2CO3 solution gave 2-hydroxymethyl (2, 3-O-isopropylidene-D-ribose)(2). Which was tosylated by Tosyl chloride in pyridine to yield compound (3), SN2 reaction of (3) with sodium cyanide in DMSO afforded compound (4). The [2+ 3] cycloaddition reaction of (4) with sodium azide gave the targeted compound (5). All prepared compounds have been characterized by: TLC, Specific rotation, Microelemental analysis and [FTIR and 1 H NMR spectroscopy]
Bioremoval of chromium from wastewater of tannery factory in Iraq was studied. The bacteria Proteus vulgaris 7E showed an enhanced capability in biosorping chromium when its concentration increased in the solution, reaching a maximum of 476,7 mg/ ml out of 492 mg/ ml under optimum conditions at pH 6 and 50°C at one hour contact time and biomass of 1 mg/ml. The present results showed that dead cells of P. vulgaris 7E biosorbed 87.41 mg/ml of chromium in comparison with91.18 mg/ml of chromium biosorbed by living cells, this indicates the insignificant effect of physiological state of cells. It was found that the above biosorption is physico-chemical process depends upon electrostatic attraction forces. The results has illustrated that the
... Show MoreThe Quantitative high-resolution planktonic foraminiferal analysis of the subsurface section in three selected wells in the Ajeel Oil Field (Aj-8, Aj-12, and Aj-15) in Tikrit Governorate, Central Iraq has revealed that Shiranish Formation deposited in Late Campanian- Latest Maastrichtian age. This formation consists mainly of marly and marly limestone yielding diverse planktonic foraminiferal assemblages and calcareous benthic foraminifera, with a total of 46 species that belong to 23 genera, Three zones and four subzones, which cover the Late Campanian to the Latest Maastrichtian, were identified based on the recorded planktonic foraminifera and their ranges. They are as follows:1. Globotruncana aegyptiaca Zone that dated to be Lat
... Show MoreTwo specie's of terrnitls are described as new to science from Iraq Amitermes corpnlentus sp. n. and Anacanthotermes savvensis sp. n., with two figures.
single and binary competitive sorption of phenol and p-nitrophenol onto clay modified with
quaternary ammonium (Hexadecyltrimethyl ammonium ) was investigated to obtain the
adsorption isotherms constants for each solutes. The modified clay was prepared from
blending of local bentonite with quaternary ammonium . The organoclay was characterized
by cation exchange capacity. and surface area. The results show that paranitrophenol is
being adsorbed faster than phenol . The experimental data for each solute was fitted well with
the Freundlich isotherm model for single solute and with the combination of Freundlich-
Langmuier model for binary system .
Gastropod species belonging to Qamchuqa Formation (Aptian-Cenomanian) in the North of Iraq are studied. These species are Harpagodes nodosus (J.de C. Sowerby,1823), Ampullina sp.1 (Hannaa and Furisch, 2011), Ampullina sp.2 (Hannaa and Furisch, 2011), Tylostoma pallaryi (Peron and Fourtau, 1904), Calliomphalus orientalis (Douville,1916), and Pyrgulifera (Meek, 1871). They are recorded in the Upper Cretaceous succession of Northern Iraq for the first time. Most of these species are internal moulds and not abundant at any stratigraphic level but irregularly scattered in the formation.
Adsorption experiments were carried out using two different low-cost sorbent materials, date seeds and olive seeds. These sorbents used as a single phase (not as mixture) to remove cadmium ions from simulated wastewater by adsorption process. The equilibrium time was found at 2 hr. The experiments include different parameters such sorbent type and weight and contact time. It was found that both of olive seed and date seed have approximately the same adsorption capacity (qm) with 15.644 mg/g and 15.2112 mg/g, respectively. Equilibrium isotherms and kinetic studies have been carried out. Langmuir isotherm model better fits the experimental data compared with the Freundlich isotherm for olive seed, while Freundlich isotherm fits for date se
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