The presence of hydrocarbons in the soil is considered one of the main problems of pollution. In our current study, eight samples isolated from soil saturated with hydrocarbons were taken from different areas of Baghdad, Iraq. In this study, 5 isolates belonging to Pseudomonas aeruginosa by 99%, 4 isolates to Klebsiella pneumoniae by 98%, and 3 isolates to Enterobacter hormaechei by 97% were diagnosed in different ways. A molecular examination was also conducted by 16sRNA. We recorded P. aeruginosa, K. Pneumoniae and E. hormaechei as new local isolates in NCBI. In addition, a comparison was made between our isolates and the global isolates to determine the degree of convergence in the evolutionary line. The genes alkB and nahAc7 were diagnosed in P. aeruginosa capable of degradation hydrocarbons. The aim of this study was to identify the bacterial species that resist the presence of hydrocarbons in the soil and also to diagnose some genes in the bacteria responsible for degradation of hydrocarbons in order to find the biological treatment methods.
The Escherichia coli isolated from UTIs, were identified with biochemical tests and vitek test.The ethanolic extracts of Zingiberofficinale(Z.officinale) were tested against E.coli by using the good agar diffusion test, the alcoholic extracts from (25 -100) mg/ml showed antimicrobial activity against tested microorganism. The diameter of inhibition zone increase at high concentrations and rang from (18-20mm) , and these results compared with antibiotics sensitivity discs were used by discs diffusion method against E.coliisolates, they were resisted to all antibiotics used in this study. It could be concluded that alcoholic extract of Z.officinalehad good antimicrobial effects, and may be able to use for treatment UTIs caused by E.c
... Show MoreCurrent study obtained (75) isolate of Pseudomonas aeruginosa collected from different cases included : 28 isolates from otitis media, 23 isolates from burn infections, 10 isolates from wound infections, 8 isolates from urinary tract infections and 6 isolates from blood, during the period between 1/9/2014 to 1/11/2014
The result revealed that the tox A gene was present in 54 isolates (72%) of Pseudomonas aeruginosa. The gel electrophoresis showed that the molecular weight of tox A gene was 352 bp. The result shows 17 isolates (60.71%) from otitis media has tox A gene, 1
... Show MoreOut of 150 different specimens, 67 S. aureus isolate were isolated. However, 16sRNA gene was located only in 60 isolates. Moreover, mecA gene was located in 48 isolates; thereby MRSA covered 80% of all S. aureus isolates. Of considerable interest, pvl gene was detected in only six isolates (10%). Hence, the present work emphasizes the notion suggested that pvl is not an indicative of CA-MRSA.
Atotal of 551 water samples drinking( five Water Treatment Plans (WTPs) and raw water( from different sites on Tigris river) were collected.According to morphological characteristics and a set of biochemical tests, one hundred twenty eight of Aeromonas spp isolates were obtained In this study The percentages of Aeromonas recovery from river water was 72.52%, from wells water was (35%).Total percentage of positive aeromonas samples of treated water(Filtration &chlorine tank, supply water of WTPs, distribution system, reserviores and other samples not related to WPTs) was 8.8%.Count of Aeromonas in positive aeromonas samples ranging from 1 to 175 cfu 100 ml.
The results showed that generally no significant correlation between presen
For the first time in Iraq, the crustacean Ergasilus ogawai Kabata,
1992 was recorded from the gills of Silurus triostegus, Mastacembelus mastacembelus, Mystus pelusius and Acanthopagrus latus out of 12 fish species caught from Garmat Ali river north of Basrah city during the period from September 1999 till August 2000. The percentage incidence of infestations of these four fish species were 98.9%, 100%,
49.6% and 71.4% while the intensity of infestations were 417, 81.8,
3.4 and 2, respectively. No significant differences in infestations of
male and female hosts with this crustacean were detected.
Summary:
Background: This study was conducted to isolate and identify the bacterial isolates of neonatal septicemia in a Neonatal Intensive Care Unite in the Baghdad teaching hospital and the resistance of these locally isolates to different antibiotics. Suspected cases of neonatal sepsis which were not treated with any antibiotics were enrolled.
Methods: One to two milliliter of blood specimens were collected from these neonates aged from 3-7 days and cultured in Brain heart infusion broth.
Results: Out of 90 neonates, 15 ( 15.5%) were positive on blood cultures. Results showed that nine isolates have been obtained belonging to Klebsiella spp. comprising (60%) out of the total isolates followed by Staphylococcus aureus (27.7%)
The present study introduces description of a new species of genus Arboridia Zakhvaticin 1946, based on a large collection of Cicadellids. External morphological characters particularly male genitalia were discussed and illustrated. The genus Arboridia Zalchvatkiia (Typhlocybinae: Erythroneurini) contains small slender, fragil and attractively coloured and patterned leafhoppers. It was erected by Zakhvatkin in 1946 (Zalchvatkin, 1946). The overall length of adults ranges from 2.5 to 3.4 mm. Members of this genus can be recognized by inner apical cell of forewing which is long with oblique base; Cu confluent with this base at a point near the middle of the length of inner apical cell; two prominent circular deep brown spots on vertex (Zal
... Show MoreThe genetic diversity was studied in sixteen barley Hordeum vulgar L. species cultivated in Iraq , which are differ in their ability to drought stress tolerance by using random amplified polymorphic DNA polymerase chain reaction (RAPD - PCR ) .Barley species was evaluated to drought stress after treatment the plant seedling at germination stages to different concentration of polyethylene glycol (PEDG6000) . The results showed that the Broaq and Arefat species have the highest tolerance to drought stress in contrast the rest of Barly species like Alkhair, Alwarkaa, Ebaa99, Shoaa, Alrafidain,Sameer Rehana 3 , forat9 , jazeral ,and ebaa7 revealed sensitivity to drought stress . The primes which used RAPD technique
... Show MoreDuring the prior three decades numerous research works presented to investigate the behavior of reinforced soil. A, IJSR, Call for Papers, Online Journal