ABSTRACT The antibacterial and antbiofilm activities of water extract of Calendula officinalis flowers against some of enteropathogenic bacteria was studied, also phytochemical screening and determination of antioxidant activity of the extract has been investigated. The results showed that the water extract of C. officinalis exhibited a good antibacterial activity against all pathogenic bacterial isolates (Salmonella, Shigella dysenteriae, Shigella flexneri, Shigella sonnei and E. coli) especially at concentration 100 µg/ml in contrast with the control cefotan antibiotic. S. sonnei was more sensitive to extract than other bacteria with highest inhibition zone (23 mm). The preliminary phytochemical tests results indicated the presence of alkaloids, saponins, flavonoids, terpenoids, glycosides and phenols, while tannins and reducing sugars absence in the extracts. Water extract (at concentration 100 µg/ml) caused 74.6% lipid peroxidation inhibition of linoleic acid emulsion, this activity was greater than other concentrations (25 and 50 µg/ml) and standard α-tocopherol (63%). Also, it was found that aqueous extracts decreased the adherent growth of bacteria on glass tubes. The results indicated that all isolates have the ability to form biofilms with different thickness degrees, the absorbance values were ranged between (1.04 - 1.68), the Salmonella isolate was the best isolate formed biofilm with highest absorbance value (1.68). On the other hand C. officinalis extract inhibited bacterial adhesion on polystyrene surface and consequently caused biofilms detachment and this revealed decreased in absorbance values of biofilms. These reported activities for C.officinalis flowers extract allow their listing as potential antibiofilm, antibacterial and antioxidant natural agents. This might suggest their use as therapeutic agents for treatment enteric infections.
Thiazoles are heterocyclic nitrogenous compounds, which were given a great attention due to their antibacterial effect, antihypertensive, stimulating glucose absorption (like antibiotics), acts as cytoprotective agent. The present work is conducted to evaluate the degree of antibacterial activity on the growth activity of Escherichia Coli (E.Coli) and Pseudomonas aeruginosa (P. aeruginosa) by incubation with 2-methyl-4-(3,4 dihydroxy phenyl)-Thiazole compound (MDHPT). Optical density (O.B) was measured by turbidmetric method, any increase in O.D would represent the increase in the bacterial growth .The results showed that there was a 75.7 % inhibition when using 190.4 ïg/ml (MDHPT) solution and 64.3% was obtained when using concentr
... Show MoreWater saturation is the most significant characteristic for reservoir characterization in order to assess oil reserves; this paper reviewed the concepts and applications of both classic and new approaches to determine water saturation. so, this work guides the reader to realize and distinguish between various strategies to obtain an appropriate water saturation value from electrical logging in both resistivity and dielectric has been studied, and the most well-known models in clean and shaly formation have been demonstrated. The Nuclear Magnetic Resonance in conventional and nonconventional reservoirs has been reviewed and understood as the major feature of this approach to estimate Water Saturation based on T2 distribution. Artific
... Show MoreCarbamazepine (CBZ) is a narrow therapeutic index drug used in the treatment of trigeminal neuralgia and psychiatric disorders. Valerian (VAL) is a popular herbal product which should be prescribed to treat insomnia and anxiety. The study was designed to investigate the presence of significant pharmacokinetic (PK) interaction between Valerian (VAL) at different concentrations on Carbamazepine (CBZ) pharmacokinetic parameters in healthy male rabbits. In an in vivo, parallel-randomized controlled trial, the rabbits in three groups "first (control), second and third" were given oral doses of CBZ (50 mg/kg), for "second and third" groups (as test groups) rabbits were given (20 and 40 mg/kg/day) of the VAL respectively, as suspensi
... Show MoreAnastatica hierochuntica L. is distributed throughout Arabain Peninsula, and elsewhere it is locally called "Kuffe Maryam" .All parts of the plant are used in folk medicine. This study amid to investigate the effect of aqueous extract of anastatica hierochunctica L. on the cancer cell lines AMN-3. Anti cancer activity of aqueous extract of anastatica hierochunctica L. showed anticancer activity against AMN-3 cell line for twelve concentrations (0.04, 0.09, 0.195, 0.39, 0.78, 1.56, 3.125, 6.25, 12.5, 25, 50, 100) mg/mL in comparison with negative control.
Methotrexate (MTX) is a folate antagonist widely used in the treatment of neoplastic diseases; its biotransformation in the liver produced active metabolites that promote hepatotoxicity. The present study was designed to evaluate the hepatoprotective effect of aqueous extract of Camellia sinensis (Green tea) against MTX-induced liver damage in rats. A model of liver injury in rats was induced by intraperitoneal injection of 20mg/kg MTX as a single dose followed by saline and 1.25% and 2.5% aqueous extract of green tea (GTE) were orally administered 7 days prior and 5 days after MTX-intoxication as a sole source of drinking water. After killing the animals, blood samples were obtained for evaluation of serum levels of alanine and
... Show MoreThe flavonoid rutin is the main content of the a queous extract of
the Iraqi plant Alhagi graecorum (family Leguminosae) which was
given to patients with different dental diseases and gave successful
results In this investigation , the amount of rutin is determined and was
found to be 4mg/100gm of the dried plant and it is equivalent to
4mg/500ml (0.8mg/100ml) of the mouthwash formula which was
prepared in this study This study reported for the first time the expiration date of rutin
in the prepared formula and was found to be 2.1 years. It also reported
for the first time that the ionic strength, buffer type and buffer concentration had no effect on the hydrolysis of rutin. Photochemical decomposition of
Anastatica hierochuntica L. is distributed throughout Arabain Peninsula, and elsewhere it is locally called "Kuffe Maryam" .All parts of the plant are used in folk medicine. This study amid to investigate the effect of aqueous extract of anastatica hierochunctica L. on the cancer cell lines AMN-3. Anti cancer activity of aqueous extract of anastatica hierochunctica L. showed anticancer activity against AMN-3 cell line for twelve concentrations (0.04, 0.09, 0.195, 0.39, 0.78, 1.56, 3.125, 6.25, 12.5, 25, 50, 100) mg/mL in comparison with negative control.
fhe .rudy has shad a light on the abi_ lity Qf aq,ueous. extract of
Mairlr:aria chamomile ibr eliminating growth of miconazole. .resistant
muta.nt of Candida albk·afzy. whi-ch i:,solated from . human. ruiil and vagina ;Tfl- inimJ.tJTI inhibition cohc!:lhtra_tion (MIC) is l6 Jrl'g/n: l. Spon-taneous tesistant .mutatiqns-.fot mic-onazole were also is:ola ed by using sp 1-f.g/ml of rni:comlzoJeThis ::onc ntrf}:ti:o:n is five times more than the MIC .Different c<:n'lcentnrtlo·ns. of aqu pus e trac.t of Mchr:ztno}Jiile'Â
... Show MoreThe effect of the aqueous extract of fig (Ficus carica) fruit was studied by using different concentrations of this fruit (15, 20 and 25 mg/kg of body weight) on some hematological parameters (hemoglobin concentration, packed cell volume, white blood cell count and platelets count) in female rabbits. Twenty female rabbits were randomly divided into four groups (five animals in each group). Three groups were dosed with the concentrations mentioned above, while the last was administered with distilled water and considered as control group. These animals were orally dosed by aqueous extract using a micropipette. The results showed that there was a significant (P<0.05) increase in hemoglobin concentration and packed cell volume means for all
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