The alterations in glyoxylate reductase and hydroxy-pyruvate reductase concentrations in the sera and the genetic alterations associated with calcium oxalate kidney stones in Iraqi patients were not studied previously so this study aimed to focus on these points. This study included 80 subjects; they were 50 patients with calcium oxalate stones compared to 30 apparently healthy controls. Biochemical investigations for kidney functions (creatinine, urea, and uric acid), were performed on the sera of both groups. Also, complete blood count, random blood sugar, and blood group tests. Furthermore, urine had been collected for General Urine Examination to visualize oxalate crystals in the urine of the patient. Also, the GRHPR enzyme concentration was measured by ELISA for both groups. The DNA was isolated from whole blood and the target DNA was amplified by PCR then the pathogenic mutations at c.295C>T (rs119490108), c.165G>A (rs180177314) and c.904C>T (p. Arg302Cys) rs180177322 were investigated by direct sequencing of the product, and then the results were analyzed. This study found that the concentration of the enzyme in the controls (4.78 ± 1.06 mg/dl) was significantly higher than its concentration in the patients (0.411 ± 0.02mg/dl). The pathogenic mutations were not found in both studied groups, but other positions were found polymorphic; at exon 4 the rs2768659 (A>G), rs1294628807 (G>A) and rs2736664 (C>T), at exon 6: c.579A>G (p. Ala193=) rs309458 and c.494-68A>G rs309459 and at exon 9 c.*146A>G rs1057507. In conclusion, this study found that calcium oxalate stones were associated with decrease GRHPR enzyme concentration in the patients compared to the control group which may be caused by mutations or epigenetics masking of the gene expression.
Objectives: To study the prevalence of rs1799964 (-1031 T/C) and rs361525 (- 238 G/A) SNPs and their effect on the disease activity, severity, and cytokines production in newly diagnosed Iraqi rheumatoid arthritis patients. Patients and Methods: sixty-three patients were diagnosed by a specialist physician while attending the rheumatology unit and twenty control participated. The inflammatory markers were measured and PCR amplification and sequencing were performed to demonstrate TNF-α SNPs. Results: Regarding (-1031 C/T) SNP, the TT genotype and allele C were significantly present in the controls, and the CT genotype was distributed significantly in the patients. The TT genotype was mostly distributed in the mild-moder
... Show MoreBackground: It was found that the occurrence of Guillain-Barre Syndrome (GBS) varies in respect to
the month and season; this variation has not been adequately studied.
Objective: To determine the month and seasonal variation of GBS in children in the Khuzestan
province, south west Iran, during a 10-year period (2006-2015).
Patients and methods: We extracted data retrospectively from acute flaccid paralysis committee
from healthcare centers in all cities of the province, over a 10-year period (from January 1, 2006 to
December 31, 2015). In this study all children under 15 years of age with Guillain-Barre syndrome
were surveyed. In order to compare the frequency of GBS in different seasons and months we used the
Ch
The problem with the research essential questionably is: (What are the variations phenotypic designs decorative Alangat) The research aims detection of phenotypic variables and Acgalah in Wares structure decorative employee in the decorative designs in the external and internal interfaces to the tombs of the Iraqi holy shrines and the walls of b (upper threshold _ Najaf) Alattabatin Husseinia and Abbasid in the holy city of Karbala) current position (1435 AH / 2014 AD). And ensure that the theoretical framework topics following: diversity in the structure motifs and vegetable processors color as well as landmarks decking decorative decorative gift items and adopted a researcher at the procedures purely on the descriptive analytical appro
... Show MoreLeishmania parasites reproduce wherever there are cells of the mononuclear phagocyte system, almost in macrophages. These are most copious in the liver and spleen;therefore, infection leads to an expansion of both of them. This study determined the burden of visceral leishmaniasis (VL) infection on liver and spleen. A total of 20 mice were infected peritoneally with 2x107promastigotes of Leishmania donovani / ml and other 12 mice left without infection as a healthy control. The weight of whole body, liver and spleen were measured and the histological development using hematoxylin and eosin stains were determined after 15, 30, 45-and 60-days post infection. The results represent that the mean weights of liver and spleen were increased in inf
... Show MoreThe relation between anemia and inflammatory immune response has lately had much attention. This research was conducted from October 2018 until April 2019, including (110) children below 12 years from both gender in some Hospitals, Primary Health care centers, Public Primary Schools and Kindergarten in Baghdad, Iraq. The objective of this study is to determine the possible correlation between iron deficiency anemia and inflammatory immune response among children infected with Entamoeba histolytica or Giardia lamblia. Blood samples were taken from all groups to measure hemoglobin level, serum iron, total iron binding capacity (TIBC), mean corpuscular volume (MCV), and mean corpuscular hemoglobin concentration
... Show MoreRheumatoid arthritis is a chronic, progressive, inflammatory autoimmune disease of unidentified etiology, associated with articular, extra-articular and systemic manifestation that require long-standing treatment. Taking patient’s beliefs about the prescribed medication in consideration had been shown to be an essential factor that affects adherence of the patient in whom having positive beliefs is an essential for better adherence. The purpose of the current study was to measure beliefs about medicines among a sample of Iraqi patients with Rheumatoid arthritis and to determine possible association between this belief and some patient-certain factors. This study is a cross-sectional study carried out on 250 already diagnosed rheumatoid
... Show MoreBackground: Diabetic Mellitus is considered as a public health concern. More than 8 percent of the United States has diabetes. Diabetes is a serious risk factor for Atherosclerotic cardiovascular disease (ASCVD) and an important cause of mortality. ASCVD is the commonest cause of death in the Western world. Diabetes was defined as a high risk condition for ASCVD. In adults with diabetes with ASCVD or multiple ASCVD risk factors it is important to prescribe high intensity statin to reduce LDL at least to 50%.
Objective: To investigate association between dyslipidemia and HbA1c and to detect benefit of using some statins in decreases the risk of CVD.
Material and method: A
... Show MoreA skin condition known as chronic spontaneous urticaria (CSU), which lacks an obvious triggering factor, is characterized by recurrent transient wheals, angioedema, or both for longer than six weeks. the current study was focused to study compared and diagnosis CSU by using heamatological and immunological parameters as (White blood cell count, eosinophils (EO), IL-6 and Total-IgE, in Chronic Urticaria patients were Clinically diagnosed by dermatologists in Specialized Center of Allergy in Baghdad/Al-Rusafa with age ranged between (11-60), as well as a control group included 40 with age ranged between (11-60). The results of demographic and clinical characteristics displayed the rate of female patients was higher than male which repr
... Show MoreProtein bound fucose (PBF), protein bound hexose (PBHex), and total calcium {T.Ca) were 'determined in sera of (40) hy-p.ertnyroidism , (40) hypothyroidism patients and (40) controL The resultsr vealed a significant decrease in the kwel of PBF, PBHex and T.Ca in sera of patients with .hyperthyroidism compared to control; Inc se Qf PBF. there nQ difference in its level betwe.en patients with hypothyroidism and control group. While there is a significant increment in PBHex leveli:n both hyper and hypothyroidism With respect to that of control Result indicates, that total calcium levels were i.n the nomml range for all p tients groups. Patient compa
... Show MoreThe present study aimed to investigate the histological changes of heart, lung, liver and kidney which caused by different concentrations (10, 20 and 40 mg/kg) of Ivabradine. Results of the study revealed some histological changes represented by aggregation of the lymphocytes around respiratory bronchioles of the lung. In the liver, the drug caused hepatocyte necrosis and infiltration of the lymphocytes. In Kidney, there are no histopathological modifications in the tissue after the animals treated with 10 mg\kg of Ivabradine. When the animals treated with Ivabradine drug at 20mg/kg of bw, dose showed vascular congestion between myocardial fibers of heart. Emphysematous c
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