A simple reverse-phase high performance liquid chromatographic method for the simultaneous analysis (separation and quantification) of furosemide (FURO), carbamazepine (CARB), diazepam (DIAZ) and carvedilol (CARV) has been developed and validated. The method was carried out on a NUCLEODUR® 100-5 C18ec column (250 x 4.6 mm, i. d.5μm), with a mobile phase comprising of acetonitrile: deionized water (50: 50 v/v, pH adjusted to 3.6 ±0.05 with acetic acid) at a flow rate 1.5 mL.min-1 and the quantification was achieved at 226 nm. The retention times of FURO, CARB, DIAZ and CARV were found to be 1.90 min, 2.79 min, 5.39 min and 9.56 min respectively. The method was validated in terms of linearity, accuracy, precision, limit of detection and limit of quantitation. The developed method was successfully applied for the estimation of furosemide, carbamazepine, diazepam and carvedilol in bulk and in pharmaceutical dosage forms.
New simple and sensitive spectrophotometric methods for the determination of paracetamol in aqueous medium were developed. The first method is based on coupling of paracetamol with p-amino-2-hydroxy sodium benzoate (AHB) in the presence of sodium periodate, as oxidizing agent, to form a brownish-orange compound which shows a λmax at 470 nm. The molar absorptivity (εmax) of the colored product was found to be (3371) l.mole1.cm-1 and Sandel’s index 0.0449 μg.cm-2. The method follows Beer’s law in the concentration range of 12.5-500.0 μg of paracetamol in a final volume of 25 ml (0.5-20.0) μg.ml-1 with relative standard deviation percent (R.S.D%) ranged between 0.26-4.71% and accuracy, expressed by recover
... Show MoreA rapid and sensitive method for analysis of amino acid hydrolysates of nigella sativa L seed has been developed using O-phthaldialehyde(OPA ) as a pre-column derivatizing agent. OPA reagents in the presence of mercaptoethanol react rapidly with primary amino acids ( less than 60 sec.) to form isindole derivatives which easily separated with good selectivity on ODS column. Resolution of amino acid derivatives is carried out with a methanol gradient in 0.01 maqueous sodium acetate. pH 7.1 . The quantitation of amino acid derivatives is reproducible within an average relative deviation of + 1.4% the linearity for most amino acids were more than 0.9993 with detection limit of 0.2 ppm. 15 amino acid were detected in the analysis of
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The extracting of personal sprite from the whole image faced many problems in separating the sprite edge from the unneeded parts, some image software try to automate this process, but usually they couldn't find the edge or have false result. In this paper, the authors have made an enhancement on the use of Canny edge detection to locate the sprite from the whole image by adding some enhancement steps by using MATLAB. Moreover, remove all the non-relevant information from the image by selecting only the sprite and place it in a transparent background. The results of comparing the Canny edge detection with the proposed method shows improvement in the edge detection.
Iris detection is considered as challenging image processing task. In this study efficient method was suggested to detect iris and recognition it. This method depending on seed filling algorithm and circular area detection, where the color image converted to gray image, and then the gray image is converted to binary image. The seed filling is applied of the binary image and the position of detected object binary region (ROI) is localized in term of it is center coordinates are radii (i.e., the inner and out radius). To find the localization efficiency of suggested method has been used the coefficient of variation (CV) for radius iris for evaluation. The test results indicated that is suggested method is good for the iris detection.
Leucine aminopepotidase (LAP)[EC:3.4.11.1] activity has been assayed in (50) serum samples of patients with diabeties naphrophathy D.N (non-insulin dependent diabetic (NIDD) , and (50)serum sample of healthy individuals without any clinically detectable diseases have been as control group. The aim of this study is to measure leucine aminopeptidase activity and partially purifying the enzyme from sera of patients with diabetes nephropathy The results of this study revealed that Leucine aminopeptidase (LAP) activity of nephropathy patient’s serum shows a high signifiacant increase (p < 0.001) compared to that of the healthy subjects.LAP was purified from the serum of patients with diabetes nephropathy by dialysis and gel filtration (Se
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Nonlinear regression models are important tools for solving optimization problems. As traditional techniques would fail to reach satisfactory solutions for the parameter estimation problem. Hence, in this paper, the BAT algorithm to estimate the parameters of Nonlinear Regression models is used . The simulation study is considered to investigate the performance of the proposed algorithm with the maximum likelihood (MLE) and Least square (LS) methods. The results show that the Bat algorithm provides accurate estimation and it is satisfactory for the parameter estimation of the nonlinear regression models than MLE and LS methods depend on Mean Square error.
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In this paper the wind data that is measured for 12 months (January to December 2011) at Al-Hay district of Wasit province, southern IRAQ country has been analyzed statistically. The wind speed at heights of 10 m above ground level was measured for every 10 minutes interval. The statistical analysis of wind data was performed using WAsP software which is based on Weibull distributions. The Weibull shape and scale parameters is obtained and used in this paper statistics. The achieved results demonstrated that the study area has Annual Mean Energy Production (AMEP) about 219.002 MWh. The computations have been performed on 70m hub‟s height of the turbine and on Earth surface roughness length (0.0, 0.03, 0.1, 0.4, 1.5) m respectively.