Klebsiella pneumoniae have an ability to form biofilm as one of strategies to persist and overcome host defenses. The study aims to evaluate the effectiveness of rosemary essential oil alone and in combination with some antibiotics against biofilm of K. pneumoniae isolated from urine. The antibiotics resistance pattern by disc diffusion method and minimal inhibitory concentration (MIC) of gentamicin, ciprofloxacin, amoxicillin, trimethoprim/ sulfame- thoxazole, cefotoxime and rosemary essential oil were determined. The ability to form biofilm as well as inhibition of biofilm formation of K. pneumoniae was performed. MICs 128, 0.25, 768, 64, 384 and 10 µg/ml were used. The effect of MIC and 1/2 MIC of antibiotics and rosemary essential oil that used alone and in combinations found significant differences (P ≤ 0.05) in biofilm reduction percentage of all isolates in comparison to control. In conclusion, the combination of antibiotics with rosemary essential oil effect negatively on biofilm of K. pneumoniae.
This research was conducted under Lath house canopy of a nursery circumstances, Baghdad University for season 2013 in order to study the effect of soak lemon seeds sour orange with different levels of licorice concentrations extract (0, 0.5, 2.5 and 4.5 g/l) for 24 hours in the percentage of germination and some recipes vegetative growth of seedlings .the results outweigh the bitter orange seedlings and focus soak for licorice extract 4.5 g / l significant superiority in most of the traits plant height, leaf number, root length, stem diameter, amounting to 31.077 cm, 14.46 paper / plant, 25.35 cm, 0.52 mm respectively, which showed significant differences for the treatment of comparison which gave lower values. Clear that the licorice ext
... Show MoreThe laboratory experment was conducted to study the effect of H2O2 with 5ppm,10 ppm and Uric Acid with 5ppm,10 ppm concentration on seed germination and seedling growth of Zea Mays L.
The result showed that the H2O2 and Uric Acid effect significantly to increase percentage of seed germination , seedling vigor index,dry weight and proline concentration compared with control treatment ,given the concentration of each H2O2 and Uric Acid of the best result of seed germination 70.00%,76.00% and seedling vigor index 21.00%,26.00% and dry weight give in 5ppm to Uric Acid significantly increased 0.34%,the 10 ppm to H2O2 increased proline concentration in plant compared with control treatment
The experiment was carried out in College of Agricultural Engineering Sciences, University of Baghdad during November 1, 2019 to June 1, 2020. The experiment was designed according to a randomized complete block design. Each block contained 18 experimental units that included the three study factors. The first factor interaction between two inoculum densities and application methods, three levels control, treatment of seeds inoculation with 5g plant and treatment of seedlings inoculation whith 30g plant . The second factor was three -1 -1concentrations of spraying of kinetin 0, 75 and 150 mg L , and third factor include two levels of organic manure) and addition of fertilizer 2% of -1 the weight of the soil (O2). The results showed a signif
... Show MoreThe experiment was carried out in the field of botanical garden belonging to the Department of Biology Sciences, College of Education for Pure Science -Ibn AL-Haitham ,Baghdad University. for the growing season. 2014 -2013 to study the effect of the electromagnetic field which included five different intensities (0,5,10,15,20) MT and three periods of time, namely, (1,2,3) an hour and their interaction on some of the morphological characteristics of the safflower plant . designed experiment by Randomized Complete Block Design (RCBD) and three replicates per treatment, compared to the average using less significant difference at the level of probability (0.05) , the results showed the following:- 1-Exposing seeds to diffe
... Show MoreAccording to the extraction procedure , Eucalyptus incrassata leave sample yielded 5% and 2% w/w(Based on dry leaves ) of the aqueous extract and essential oils respectively. Disk diffusion method was used to determine the antimicrobial activity of aqueous extract and essential oils of E . incrassata leaves against eight isolates of multidrug- resistant of Staphylococcus aureus ( S. aureus) . It was found that aqueous extract and essential oils have variable antimicrobial activity(the inhibition zone diameter ranged from 7 to 14 mm respectively ) , while essential oils showed more effect than aqueous extract . It was noticed that values of Minimal Inhibitory Concentration ( MIC ) for
... Show MoreThe effect of thickness variation on some physical properties of hematite α-Fe2O3 thin films was investigated. An Fe2O3 bulk in the form of pellet was prepared by cold pressing of Fe2O3 powder with subsequent sintering at 800 . Thin films with various thicknesses were obtained on glass substrates by pulsed laser deposition technique. The films properties were characterized by XRD, and FT-IR. The deposited iron oxide thin films showed a single hematite phase with polycrystalline rhombohedral crystal structure .The thickness of films were estimated by using spectrometer to be (185-232) nm. Using Debye Scherrerś formula, the average grain size for the samples was found to be (18-32) nm. Atomic force microscopy indicated that the films had
... Show MoreAn experiment was carried out by using post in kalar horticulture Station/Sulaimania province on soil taked from once region sields during growing season of 2008-2009. The objective was to study adding increasing levels of urea fertilizer which is (0.0, 0.20, 0.40, 0.80) gm/Pot and superphosphate fertilizer which is (0.0, 0.24, 0.48) gm/pot in some chemical properties of grain for wheat IPA 95. This experiment was carried out by completely randomized design (CR D) with three replications. Results in dictated of clear increase in all the studied characteristics (concentration for each nitrogen, phosphorus and potassium and carbohydrate percentage with increasing levels of fertilizers).
Seventy-six urine specimens were collected from of patients suffering from recurrent
urinary tract infections (UTIs). Specimens were bacteriologically analyzed, fifty
(65.8%) of isolated bacterial strains were belonged to E.coli. 100% of isolated
uropathogenic E.coli (UPEC)strains displayed a biofilm positive phenotype under
optimized condition using microtiter plate assay. 21 of E.coli strains classified as highly
positive biofilm producers (42%), and 29 (58%) as weakly positive biofilm producers.
This study aims to investigate the effect of low concentrations of sulfuric antioxidants on sperm of rams. This study was conducted in the field of sheep and goats of the Department of Livestock in the collage of Agricultural Engineering Sciences, University of Baghdad. The semen was collected using the artificial vagina at weekly rate (Pooled semen) and was subjected to different tests and then was divided into seven treatments but different concentrations of cysteine (0.5, 1mM) and glutathione (0.5, 1mM) and taurine (0.5, 1mM).The sample of the semen was diluted with Tris dilution (10: 1). The results showed that the low concentrations of glutathione, cysteine and taurine resulted i