Oral squamous cell carcinoma (OSCC) is the most common malignant neoplasm of the oral mucosa. Human papillomavirus (HPV) virus cause a broad scope of diseases from benign to invasive tumors, types 16 and 18 classified as carcinogenic to humans. This study aimed to provide the first molecular characterization of HPV types in Iraq. Thirty-five unstimulated whole saliva samples were collected from histopathologically confirmed patients with oral cancer were enrolled in this study. Genomic DNA was extracted from exfoliating cells to amplify HPV-DNA using HPV-L1 gene sequence primers by polymerase chain reaction method (PCR), the viral genotyping was performed using direct sequencing method. HPV genotypes identified were deposited in GenBank. HPV DNA was detected in 20 of 35 OSCC patients representing (57%).The most frequent HPV genotypes were HPV-18 accounting for (75%) (15 out of 20 patients) followed by HPV-16 accounting for (20%) (4 out of 20), and HPV-11 accounting for (5%) (5 out of 20 patients). This study highlights the high-risk HPV genotypes in OSCC patients and their phylogenetic analysis tree and their homology to the ancestral sequence which may indicate emerging of a new biological entity of HPV-positive OSCC with a potential sexually transmission.
Abstract
Background
Stathmin1 (also known as metablastin) is a major microtubule-depolymerizing protein that involved in cell cycle progression and cell motility.
Stathmin1 has been found to be up-regulated in some cancers and correlated with cell differentiation and proliferation.
Stathmin1 is a major cytosolic phosphoprotein that regulates microtubule dynamics and is associated with malignant phenotypes in various cancers, including non-small cell lung cancer.
Objective
To evaluate the role of Immunohistochemical expression of stathmin1 in non-small
... Show MoreThe Hartha Formation is one of the important formations deposited during Late Campanian age.
The present study deals with four boreholes (EB-53, 54, 55 and 56) within the East Baghdad oil field to diagnoses the microfacies and interpret the depositional environments.
Six major microfacies were recognized in the succession of the Hartha Formation. Their characteristic grain types and depositional texture enabled the recognition of paleoenvironment. There are Orbitoides wackestone-packstone , Orbitoides - miliolid wackestone, Peloidal and Pellets - echinoderm wackestone to packstone, Peloidal wackestone to packstone, Pelletal wackestone to packstone, and Planktonic foraminifera wackestone-packstone.
Four assoc
... Show MoreBackground: The aim of this study was to evaluate the expression of fibroblast growth factor-2 and Heparanase in salivary pleomorphic adenoma, and to correlate the two studied markers with each other and with clinicopathological parameters including: age, sex, tumor site and histopathological presentation. Methods: Sections of twenty five formalin-fixed paraffin embedded tissue blocks specimens of salivary pleomorphic adenoma were immunostained using monoclonal antibodies (Fibroblast growth factor-2 and Heparanase) to assess their expression in this tumor. Results: The expression of fibroblast growth factor-2 and Heparanase were positive in all pleomorphic adenoma cases (100%). The positive expression of fibroblast growth factor-2 was signi
... Show MoreBackground: The level of parent education has an important role in making decision about their children oral hygiene.<br />Material and methods: A cross sectional descriptive study was conducted in Baghdad city, the total sample include 569 preschool children (318 males and 251females) aged 5-years, they were randomly selected from different kindergartens. For measuring oral cleanliness gingival condition and dental stain, Plaque index by Silness and Lِe gingival index by Lِe and Silness and stain index by Leung were used respectively.<br />Results: Results showed that the mean plaque index was (0.68 ± 0.018) results reveal a high prevalence of gingival inflammation (77%)with mild type was found to be the predominant, as m
... Show MoreBackground : From the standpoint of mortality &morbidity, cancer is by far the most important clinical problem that concerns the breast today .The age adjusted incidence of new cases has been increasing steadily with increase in the incidence among the Iraqi women during the last few years .
Materials &method : A prospective study was arranged to assess the occurrence of postsurgical loco-regional recurrence of breast carcinoma in the Iraqi female patients , a total
number of 91 female patients were assessed during period from Dec.2000 to Dec.2002, the
median period of follow up was two years.
Result : Loco-regional recurrence developed in 20 patients (22%). Chest wall and axilla were th
Leishmaniasis is a group of parasitic diseases caused by Leishmania spp., an endemic infectious agent in developing countries, including Iraq. Diagnosis of cutaneous lesion by stained smears, serology or histopathology are inaccurate and unable to detect the species of Leishmania. Here, two molecular typing methods were examined to identify the promastigotes of suspected cutaneous leishmaniasis samples, on a species level. The first was species-specific B6-PCR and the second was ITS1-PCR followed by restriction fragment length polymorphism (RFLP) using restriction enzyme HaeIII. DNA was extracted from in vitro promastigote culture followed by amplification of kDNA by B6 or amplification and digestion of LITSR/L
... Show MoreApproximately 15% of all couples all over the world suffer from difficulty conceiving their first child. The word "infertility" is used to describe this problem.
When a couple had regular, unprotected sexual intercourse for a year or longer, it indicates that they have been unsuccessful in their efforts to conceive.
The current investigation aims to find out if there is a relationship between PROTAMIN-1(PRM-1) and INTERLEUKINE-12 (IL-12) gene expressions and their effect on the development of infertility. The current investigation comprised 100 teratozoospermia patients and 100 healthy fertile controls who had their semen examined. Samples were given by Al-Nahrain University's Biotech
... Show MoreBackground: Hydatid disease is a serious infection of man caused by larval stage (hydatid) of the small dog tapeworm. Serological tests are important in the diagnosis of hydatid
disease.
Objectives: The study was carried out to detect the rate of seropositivity of hydatid cysts in Kirkuk and Tikrit cities.
Materials and Methods: The retrospective hospital based and seroepidemiological studies were carried out in Kirkuk and Tikrit cities, during the period from beginning of January
2000 until the end of October 2003. The hospital based study included 125-hydatid cyst operations in hospitals of Kirkuk (82) and Tikrit (43). The serological tests used for
detection of hydatid cysts antibody were latex agglu