The plant Conyza canadensis, which belongs to Asteraceae (Compositae) family and known as Canadian horseweed. It was used as traditional medicine in China, Pakistan, India, and Africa for the treatment of various diseases causing by bacteria, fungi, or viruses. The plant has antimicrobial, antioxidant, anticoagulant, anti-inflammatory, and anticancer pharmacological activity. This study provides the first phytochemical investigation of the plant in Iraq and is concerned with extraction, fractionation, isolation, and purification of some of the important phytochemicals detected in the plant-like phenolic acids, flavonoids, and alkaloids. Also, the literature survey has revealed that the plant has a substantial antimicrobial activity, so it was deemed desirable to make a study for the antimicrobial activity of the plant. The whole plant was collected from Baghdad city / College of Pharmacy/ University of Baghdad farm during July (2020). The aerial parts and roots were washed thoroughly, dried in shade, chopped, pulverized into a coarse powder, and then weighed. The shade-dried crushed plant materials were first defatted by maceration in hexane for 24 h. Then extracted by two extraction methods (hot method using soxhlet apparatus and cold method by maceration in solvent), using 85% aqueous ethanol as solvent extraction, and fractionated by petroleum ether, chloroform, ethyl acetate, and n-butanol. The phytochemical screening of the ethanolic extract from both extraction methods revealed alkaloids, saponin glycosides, coumarins, flavonoids, steroids, phenolic compounds, proteins, anthraquinones, terpenoids, and cardiac glycosides. However, depending on the percentage yields, the hot method yield was better than the cold method, so the extraction method by soxhlet was preferred upon maceration as it gives a higher percentage yield. The petroleum ether, chloroform, and ethyl acetate fractions were analyzed by thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC) for their steroids, alkaloids, and polyphenolic (phenolic acids and flavonoids) contents, respectively. The different chromatographic results revealed the presence of stigmasterol and β- sitosterol in petroleum ether fraction, harmine alkaloid in chloroform fraction, quercetin, quercitrin, apigenin, p-coumaric acid, and caffeic acid in ethyl acetate fraction of the Iraqi C. canadensis plant. Three polyphenolics compounds (p-coumaric acid, caffeic acid, apigenin) were isolated from ethyl acetate fraction by preparative thin-layer chromatography plates (PLC), and Harmine alkaloid was isolated from chloroform fraction by high-performance liquid chromatography (HPLC) using a fraction collector. The isolated compounds were subjected to various chromatographic and spectral analytical techniques for their identification, such as TLC, FTIR, HPLC, and high-performance thin-layer chromatography (HPTLC). Petroleum ether fraction was analyzed for the detection of coumarins by TLC. One compound was isolated, purified by PLC, symbolized as MS compound, and identified by FTIR and 1H -NMR since there is no standard available for this compound. The isolated MS compound could be pyranocoumarin glycoside. To investigate the essential oil composition of Iraqi C. canadensis, hydrodistillation of fresh aerial part of the plant was done using Clevenger-type apparatus for 3hr. The essential oils components and the hexane fraction obtained by maceration of the plant material in hexane solvent were identified using GC/MS analysis. The results of GC/MS analysis of the essential oil were abundant by hydrocarbon compounds, particularly by sesquiterpene hydrocarbon. This study also involves a preliminary determination of the antimicrobial activity of ethyl acetate fraction of the plant against two Gram-positive bacteria (Staphylococcus aureus and Staphylococcus epidermidis), two Gram-negative bacteria (Escherichia coli and Klebsiella sp.) and one fungi species (Candida albicans) by measuring the inhibition zone diameter around the hole in mm, compared with streptomycin and fluconazole standard drugs for antibacterial and antifungal activity, respectively. The antimicrobial results showed significant antibacterial activity against S.aureus (gram-positive bacteria) and important antifungal activity against Candida albicans. In contrast, no antibacterial activity was demonstrated against the tested gram-negative bacteria. Furthermore, the antibacterial activity exerted against S. epidermidis (gram-positive bacteria) was affected by dilution dimethyl sulfoxide (DMSO).
An antibacterial and antifungal piperonal-derived compound and its Rh(III), Pd(II), Pt(IV), and Cd(II) metal complexes were synthesized and characterized by spectroscopic methods, conductivity, metal analyses and magnetic moment measurements. The nature of the complexes formed in ethanolic solution was studied following the molar ratio method. From the spectral studies, octahedral geometry was suggested for rhodium (III) and platinum (IV) complexes, while a square planer structure was suggested for palladium (II) complex and a tetrahedral geometry for cadmium (II) complex. Structural geometries of these compounds were also suggested in gas phase by using hyperchem-8 program for the molecular mechanics and semi-empirical calculations.
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Dental clinicians and professionals need an affordable, nontoxic, and effective disinfectant against infectious microorganisms when dealing with the contaminated dental impressions. This study evaluated the efficiency of hypochlorous acid (HOCl) as an antimicrobial disinfectant by spraying technique for the alginate impression materials, compared with sodium hypochlorite, and its effect on dimensional stability and reproduction of details. HOCl with a concentration of 200 ppm for 5 and 10 min was compared with the control group (no treatment) as a negative control and with sodium hypochlorite (% 0.5) as a positive control. Candida albicans, Staphylococcus aureus, and Pseudomonas aeruginosa were selected to assess the antimicrobi
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Dental clinicians and professionals need an affordable, nontoxic, and effective disinfectant against infectious microorganisms when dealing with the contaminated dental impressions. This study evaluated the efficiency of hypochlorous acid (HOCl) as an antimicrobial disinfectant by spraying technique for the alginate impression materials, compared with sodium hypochlorite, and its effect on dimensional stability and reproduction of details. HOCl with a concentration of 200 ppm for 5 and 10 min was compared with the control group (no treatment) as a negative control and with sodium hypochlorite (% 0.5) as a positive control. Candida albicans, Staphylococcus aureus, and Pseudomonas aeruginosa were selected to assess the antimicrobi
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Sage (Salvia officinalis), belong to Labiatae family is indigenous to Iraq and other Mediterranean areas but now cultivated world- wide, principally for its use as culinary herb. In the present study preliminary screening for the important phytochemical natural product groups indicated the presence of flavonoid, saponin, hyrolysable and condensed tannin groups. The antibacterial activity of two concentrations 10 mg/ml and 100 mg/ml of chloroform and hydroalcoholic extracts from Salvia officinalis leaves was evaluated against four strains of gram negative bacteria (Escherichia coli, Pseudomonas arigenossa, Klebsiella pneumonia, and Proteus spp) and two str
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Two compounds,[2-amino-4-(4-nitro phenyl) 1,3-thiazole],(4) and [2-amino-4-(4-bromo phenyl) 1,3-thiazole],(5), were synthesized by refluxing thiourea (1) with each of para-ntiro and para-bomophanacyl bromides(2) and (3) respectively, in absolute methanol. Then, by reaction of [5] with 3,5-dinitrobenzoyl chloride in dimethylformamide (DMF) yielded (6) .On the other hand, reaction of (4) with chloroacetyl chloride in dry benzene afforded (7), which is upon treatment with thiourea in absolute methanol, af
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This study was aimed to produce bacteriocin from Bacillus. licheniformis isolated from local soil of corn and sunflower fields and using as antimicrobial agent . Fourteen of local isolates of Bacillus sp. were obtained and ability of these isolates for growth on Brain heart infusion agar (BHI) at 550C were tested. Isolate C4 was revealed high growth density in comparison with other isolates. Isolate C4 was identified as Bacillus licheniformis according to morphological, cultural and biochemical tests, Moreover genetic analysis for 16S rRNA gene and given accession number MT192715.1 in GenBank of NCBI . Production of bacteriocin from this isolate was carried out in Luria Broth (LB) and partially purified by precipitation with 30-70 % saturat
... Show MoreThis study deals with air pollution tolerance index (APTI) and anatomical variation in leaves of two species of terrestrial plants Ficus sp. and Conocarpus sp. that have bee commonly the separated along roadsides in many stations within Babylon province. APTI values of both species were less than 10 during study period which represented sensitivity of these plants to air pollution. There are Anatomical responses to pollution in the leaves of both studied species. Main adaptations included increased thickness of parenchyma cell walls with clear dark deposits in sections of Ficus sp. from sections of stations 2 and 4 which represent polluted stations. Conocarpus sp. main adaptation included stomata increased in density and decreased in size w
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Five isolates of Gram negative bacteria (Klebsiella pneumoniae, Psuedomonas auroginosa, proteus mirabilis and two strains of E.coli) were in quested for the ability of bearing silver nanoparticles by using LB medium, all the isolates of bacteria were buttered brown color just as soon as mixed the supernatant of bacterial culture with AgNO3 solution, that refered the biosynthesis of Silver nanoparticles (Ag NPs). UV–visible spectrophotometer and Fourier transform infrared (FTIR) spectroscopy were utilized for estimation of (Ag NPs). The five isolates of bacteria were tendered to produce spontaneous mutants by using different kinds of antibiotics, Ampicillin put to use for making mutant in E.coli and Proteus mirabillis, while Pseudom
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The primary goal of root canal treatment (RCT) is to expel the presence of any necrotic or vital tissue, microbes and their byproducts from the canal space before press forward with the following steps of the RCT procedures. Although this is difficult to attain, various strives had been practiced by employing chemical and mechanical methods to eliminate as much microorganisms as possible and make the canal space valid for the obturation materials to be received. The aim of this review is to demonstrate some of what new remedies that could be used as root canal disinfectant by summarizing the recent studies regarding the efficacy of different natural products against the most persistence microbiota that could be responsible for most
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Biological Activity of Complexes of Some Amino Acid