The present study aims to evaluate the effects of methotrexate (MTX) with and without vitamin A (Vit. A) on some biochemical parameters and histological structure in male rabbits liver. Twenty male rabbits weighing 1250-1480 gm were divided into four equal number groups. The first group was given 2 ml distilled water as control group. The second group was given MTX (20 mg/kg), the third group was given Vit. A (5000 IU), while the fourth group was given MTX (20 mg/kg) +Vit. A (5000 IU) in alternative days. Following four weeks of treatment, lipid profile total cholesterol (TC), triglycerides (TG), high-density lipoprotein (HDL), [low-density lipoprotein (LDL) and very low-density lipoprotein (VLDL)]; in addition to thyroid hormones triiodothyronine (T3) and thyroxin (T4)] and liver enzymes [glutamic pyruvic transaminase (GPT) and glutamic oxaloacetic transaminase (GOT)] were determined in the serum. Also, the histological examination of liver of all the experimental groups were carried out. The results were revealed that the treatment with MTX caused a significant P≤0.05 increases in TC, HDL, LDL, T4, and GPT when compared with the control group. The treatment with Vit. A did not cause any significant P≥0.05 differences in all the studied parameters. The MTX+Vit. A treated group showed a significant P≤0.05 increases only in GPT compared with the control group; while a significant P≤0.05 decreases was found in TC, HDL, T3, T4, and GOT when compared with the MTX treated group. The histological examination of the liver sections showed that MTX administration caused major histological changes in comparison with the control such as inflammatory cell infiltrations, vascular congestion, sinusoidal dilatation and granular degeneration of hepatocytes. Treatment with Vit. A showed a typical structure in liver tissue. While in MTX+Vit. A group, the histological changes were less severe than those in the MTX treated group; these changes were granular degeneration of hepatocytes and sinusoidal dilatation at low levels. The overall results of this study confirmed that administration of Vit. A decreased the side effects of MTX; this protective effect of Vit. A may have clinical applications in chemotherapy.
The present study aims to investigate the effects of duration and complications of type 2 diabetes mellitus on diabetic related parameters, adipocytokines and calcium regulating hormones. This study was conducted on eighty diabetic patients (41 male and 39 female) in Iraq, with an age range of 20-60 years and a disease duration range of 1-180 months. Blood samples were collected from the patients to determine three main types of biomarkers: diabetic-related parameters [Fasting blood sugar is FBS glycated hemoglobin (HbA1c), insulin, and insulin resistance (IR)], adipocytokines [adiponectin and tumor necrosis factor-α (TNF-α), and calcium regulating hormones [parathyroid hormone (PTH), calcitonin, and vitamin D].
 
... Show More
(11)
(4)
A histological study was conducted to examine the structure of rhombencephalon (Cerebellum) in Columba livia gaddi (Gmeiin, 1789). The result showed that the cerebellum is aportion of the brain lying behind the cerebrum, the surface of the cerebellum contains deep folds. The cerebellum consists of two regions a cerebellar cortex that is called gray matter composed of three major layers from the outside to the inside, as follows: molecular layer, Purkinje cells layer and granular layer, the second region of cerebellum called medulla. This is the white matter.
Previous reports demonstrated the effectiveness of silibinin hemisuccinate as a potential intraocular pressure-lowering agent. The exact mechanism by which silibinin exerted this effect has not yet been documented, but might suggested to interfere with aqueous humor formation. The present study was designed to evaluate the comparative efficacy of silibinin as IOP lowering agent to that of betaxolol in normotensive rabbits, and the interaction of silibinin with betaxolol as a way for investigating the possible mechanism of action of silibinin in this respect. The effects of instillation of 0.75% silibinin solution and 0.5% betaxolol eye drops in the eyes of normotensive rabbits were evaluated using indentation tonometry. The results showe
... Show More
This study was aimed to investigate the iron overload effect on the levels of ferritin, testosterone, follicle stimulating hormone (FSH), luteinizing hormone (LH) and prolactin (PRL) in male patients with β-thalassemia. Blood levels of sex hormones and ferritin were determined in (50 males) beta-thalassemia patients, aged (16-23) years and in (30) healthy males matched for age. They were recruited from the Abin Al-Balady Hospital in Baghdad. Ichroma™ kits were used to determine the blood levels of sex hormones and ferritin. The results showed that the level of testosterone, LH and FSH were significantly (p≤ 0.05) lower in β-thalassemia male patients compared to controls. Furthermore, the level of ferritin and prolactin in male pati
... Show MoreThe finite element method has been used in this paper to investigate the behavior of precast reinforced concrete dapped-ends beams (DEBs) numerically. A parametric investigation was performed on an experimental specimen tested by a previous researcher to show the effect of numerous parameters on the strength and behavior of RC dapped-end beams. Reinforcement details and steel arrangement, the influence of concrete compressive strength, the effect of inclined load, and the effect of support settlement on the strength of dapped-ends beams are examples of such parameters. The results revealed that the dapped-end reinforcement arrangement greatly affects the behavior of dapped end beam. The failure load decreases by 25% when
... Show More
Objective: In this study ,the effects of silver nanoparticles (Ag NPs)were investigated on the liver and kidney tissues. Methodology: The produced nanoparticles have an average particle size of about 30 nm. Eighteen male albino rats were used by dividing them into three groups, each group comprise 6 rats. First group(control group) given food and water like other groups by liberty. Second group was tail injected by (AgNPs) at dose of (0.4 mg/kg. body weight/day). Third group was injected by (AgNPs) at dose of (0.6 mg/kg. body weight/day) for 15 days. All animals were sacrified at the end of experiment. The liver and kidney tissues specimens were fixed in 10% formalin and histological preparations were carried out then stained with H&E. Path
... Show More
Methotrexate (MTX)is a multiple therapeutic drug, it's used in treatment of ectopic pregnancy, neoplastic disease, autoimmune disorders, and inflammatory conditions. It’s associated with a spectrum of side effect that includes damage to the (liver, kidney, lung and bone marrow). MTX has been associated with fetal malformations in( nervous, skeletal, gastrointestinal, and cardiac) system, and even fetal death. The study was conducted to test the effect of two doses of MTX (5and 10mg/kg) on the development of the mice liver during two gestational age (day11 and day 17) of pregnancy.18 healthy female mice were divided into three groups ,first group (control) was injected with normal saline ,second group injected with 5mg/kg and third grou
... Show MoreThe isotretinoin drug is 13-cis-retinoic acid, is the treatment of severe acne and some skin cancers and used for dermatological conditions, this study was designed to detect the toxic role of the isotretinoin on the intrauterine development after implantation in albino mice during pregnancy. There are very little studies which indicating the side-effect of this drug on intra-uterine growth, so in the present research we tried to study the toxic effect of isotretinoin on the endometrial changes of uterus during the 2nd. and 3rd. trimester of gestation in pregnant mice after treatment with single chronic dose of the Isotretinoin drug (20 mg/B.wt) from first day of gestation until 21 days the last day of gestation.
The present study exa