Background: Toxin-producing Shiga Escherichia coli has been identified as a new foodborne pathogen that poses a significant health risk to humans. Shiga toxin-producing Escherichia coli can be found in raw cow milk and its derivatives. A small number of Escherichia coli strains that produce shiga toxin are pathogenic. Aim of study: The study aimed to see if there were any virulence genes in 50 milk samples that were typical of Entero-haemorrhagic E. coli and evaluate the Myrtus communis effects on these bacteria. Materials and Method: Milk samples were used to isolate E. coli bacteria (n= 27), biochemically analyzed, and genetically screened for virulence genes using a multiplex (PCR). The hydro-alcoholic extraction of Myrtus communis leaves was tested at four strengths, ranging from 20-50 mg/ml. Results: The findings of the molecular profile indicated that (stx2) was found in 11 (40.7%),(hlyA) in 13 (48.2) and eae genes in 9 (33.3) of E. coli isolate, respectively. Treatment with an extract of this plant at a dosage of 50 mg/ml had the highest effect on Escherichia coli, which was significantly different from all other treatments. Conclusions: The virulence genes shigatoxin-2 (stx2), intimin (eae), and entero-hemolysin (hlyA) were found in Strains of E. coli isolated from milk, according to the findings of this study.
Bodies of water are usually being polluted by wastes from domestic and industrial sources thereby making them unfit for use. Hence, this study aimed at assessing the water quality from Asa River, Ilorin, Nigeria in terms of bacteriological and physicochemical parameters. The bacteriological parameters assessed were heterotrophic bacterial count, total coliform, faecal coliform, identification of the isolates, antibiotic resistance patterns, and plasmid profile of the isolates. Whereas, the assessed physicochemical parameters were pH, total chloride, suspended solid, and total hardness. The heterotrophic bacterial count, total coliform, and faecal coliform counts ranged from 7.6 x 103 to 3.2 x 106 cfu/ml,
... Show MoreAl-Chibayish Marsh (CM) is considered as the major part of Central Marshes area of this marsh is 1050 Km². The water quality of these marshes is suffering from salt accumulation due to intensive dam construction, limited supply of water from sources, climate change impacts, and the absence of outlet flow from these marshes, specifically at low flow periods. So, the current research aims to assess and improve these marshes' hydraulic behavior and water quality and define the best location for outlet drains. Field measurements and laboratory tests were conducted for two periods (November 2020 and February 2021) to define the (TDS) concentrations at nine different locations. Samples were also examined for water's phy
... Show MoreAbstract A total of 207 specimens were collected from different sources including patients, health care staff and hospital environment in Ibb city, Yemen. The study used the bacteriocin produced from active producer strains in typing of Staphylococcus aureus. Depending on the morphological, cultural and biochemical characteristics, 54 (26.09%) isolates of Staphylococcus aureus were identified. An antibiotic sensitivity test was done for the bacterial isolates, and the results showed that there were multiple resistant antibiotics. The Staphylococcin production of these isolates has been detected by using wells assay. Fifty one isolates were Staphylococcin producer. Four isolates (staph19, staph25, staph28 and staph43) were chosen as go
... Show MoreBacterial strains were isolated from oil-contaminated soil, in 2018, these isolates were identified, and with the aim of finding out the ability of these isolates to degrede the oil compounds, the color change of medium which added to it isolates was read by the method of Pacto Bushnell Hans. Then the change in the petroleum compounds was read by gas chromatography, for the most effective isolates.
The nine isolated bacterial showed different degrees of color change, and the isolates (Pseudomonas, Bacillus, Micrococcus) outperformed the color change amount (78, 78, 77) %, respectively, compared to the control, and the three isolates together showed the best color change of 90.7. % Compared to the control, and the
... Show MoreAl-Ruhbah region is located in the southwest of Najaf Governorate. A numerical model was created to simulate groundwater flow and analyze the water quality of the groundwater, by developing a conceptual model within the groundwater modeling system software. Nineteen wells were used, 15 for pumping and four for observation. A three-dimensional model was built based on the cross-sections indicating the geologic layers of the study area, which were composed of five layers. When a distance of 1,000 m between the wells was adopted, 135 wells can be operated simultaneously. These wells were hypothetically operated at 6, 12, and 18 h intervals, with a discharge of 200, 430, and 650 m
Back ground: One out of six children in the
world today is involved in child labor, doing
work that is damaging to his or her mental,
physical and emotional development.
Objective: Assessment of some health
problems among the studied working children.
Method; A cross-sectional study was
conducted in Al Amen Primary Health Care
(PHCC) during the period from January to
August 2009, a sample of 6048 children were
selected randomly(3218girls and2866 boys age
between 5-17 years ) and interviewed to collect
information using a structured questionnaire
form, information related to different aspects
of child labor prevention were included in the
form as well as a general medical examination
and lab
ABSTRACTBackground: In Medical ethics education, improving medical student’s attitudes toward respecting the right of patients is an essential task. The medical students’ attitude has been affected by social, educational and personality background factors.Objective: To investigate medical student’s attitudes regarding medical ethics courses.Method: The study was conducted in Al-Kindy College of Medicine on academic year (2013 -2014) for the period from January to September. A cross- sectional study design was adopted with a self- administered questionnaire form distributed to medical students in the 5th-6th under graduate grades. The questionnaire consisted of 31 items relevant to student’s opinion about attitudes concerning ethi
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