Background: Toxin-producing Shiga Escherichia coli has been identified as a new foodborne pathogen that poses a significant health risk to humans. Shiga toxin-producing Escherichia coli can be found in raw cow milk and its derivatives. A small number of Escherichia coli strains that produce shiga toxin are pathogenic. Aim of study: The study aimed to see if there were any virulence genes in 50 milk samples that were typical of Entero-haemorrhagic E. coli and evaluate the Myrtus communis effects on these bacteria. Materials and Method: Milk samples were used to isolate E. coli bacteria (n= 27), biochemically analyzed, and genetically screened for virulence genes using a multiplex (PCR). The hydro-alcoholic extraction of Myrtus communis leaves was tested at four strengths, ranging from 20-50 mg/ml. Results: The findings of the molecular profile indicated that (stx2) was found in 11 (40.7%),(hlyA) in 13 (48.2) and eae genes in 9 (33.3) of E. coli isolate, respectively. Treatment with an extract of this plant at a dosage of 50 mg/ml had the highest effect on Escherichia coli, which was significantly different from all other treatments. Conclusions: The virulence genes shigatoxin-2 (stx2), intimin (eae), and entero-hemolysin (hlyA) were found in Strains of E. coli isolated from milk, according to the findings of this study.
Segmented regression consists of several sections separated by different points of membership, showing the heterogeneity arising from the process of separating the segments within the research sample. This research is concerned with estimating the location of the change point between segments and estimating model parameters, and proposing a robust estimation method and compare it with some other methods that used in the segmented regression. One of the traditional methods (Muggeo method) has been used to find the maximum likelihood estimator in an iterative approach for the model and the change point as well. Moreover, a robust estimation method (IRW method) has used which depends on the use of the robust M-estimator technique in
... Show MoreThe levels of lead (pb), copper (cu), cobalt (co) and cadmium (cd) were determined in different kinds of milk and the health risks were evaluated. The mean levels were 0.73±0.21, 0.06±0.01, 0.12±0.01 and 0.14±0.01 ppm for these metals respectively. The levels of pb and cu were found to be insignificant differences (p<0.05), whereas the levels of co and cd, were no significant differences (p>0.05). The dry and liquid kinds of milk were different significantly (p<0.05), whereas the original, was no significant differences (p>0.05). The values for all metals were more than one. The metals pb and cd were detected at highest concentrations in most dry and liquid milk samples.
Underground water is extracted by wells that are connected through underground canals. Thus, the levels of pollutant elements in ground water could be evaluated directly from water samples collected from the wells. This study was conducted in the city of Samara / Salah-Aldeen province – Iraq. The samples were taken from 29
wells within the study area for the period 2012-2014 with a supervision from the General Authority for Groundwater / the Iraqi Ministry of Water Resources. GIS technology was adopted to calculate the pollution elements at Samara water table. The concentrations of chemical elements [K, Na, Mg, Cl, Ca] and compounds [SO4, HCO3] were manipulated and calculated for the entire area. The results of laboratory analy
Eight soil samples were selected around Najaf governorate at depth levels 40-50 cm. X-Ray Fluorescence (XRF) was used to determine the concentrations of major and trace elements. Liner and mass attenuation coefï¬cient (µ, µÏ) have been determined at gamma energies (662, 1172,1332) keV using NaI (Tl) detector. The range of linear attenuation coefficients for calculated samples were (0.553-1.163) cm-1, (0.122-0.178) cm-1 and (0.049-0.105) cm-1 at (662, 1172,1332) keV respectively. The range of mass attenuation coefficients obtained (0.39-0.76) cm2/gm, (0.087-0.117) cm2/gm and (0.0336-0.074) cm2/gm at (662, 1172,1332) keV respectively. The result
... Show MoreThe survey was carried out From January to April of 2018 on macrofungi samples collected from different places in Halabja province located in north eastern parts of Iraq-Kurdistan region. This region is rich in forest trees and pasture lands with diversity of shrubs and herbs and is expected to support the growth of several macro fungal species. However, this part of Kurdistan in Iraq is still unexplored from macrofungal point of view. In this paper three species from Pezizaceae and Pyronemataceae families that belonging to (Pezizales, Ascomycota), were reported from Iraqi Kurdistan. These macrofungal species are recorded for the first time from Iraq. Also the species were identified and showing their locations distributed on a map prepared
... Show MoreObjective :To evaluate elderly's environmental practices concerning fall prevention at governmental elderly care homes in Baghdad city. Methodology: A quazi- Experimental study was carried out in governmental elderly care homes at Baghdad city, during the period 1st, June 2014 to 30th November, 2014 , selected a purposive " Non – probability " sample of (40) elderly men and women aged (60) years old and over who were resident in governmental elderly care homes " Al Ceelakh and Al Sader elderly care homes", the data was collected through the use of constructed questionnaire that consist of (23) items,
Three seismic instantaneous attributes (phase, frequency, and variance) were utilized on 3D-seismic poststack migrated data, covering 617.31 km2, integrated with data of two wells (Du-1 and Du-2) in Dujaila oil field, southeast of Iraq. They gave good results in detecting reef buildups and confirmed the existence of the stratigraphic hydrocarbon trap that was not obvious in the conventional seismic amplitude sections. They display several seismic criteria in attribute sections for recognizing reef buildups and hydrocarbon accumulation, such as phase reversal, low frequency, and high amplitude variance. The seismic attributes emphasized that the stratigraphic trap of reef rudist buildups with hydrocarbon content is con
... Show MoreThe trade balance is considered as a way to join the national economy with the world, So it is the mirror that reflect the economic center of the country and it is point to competitive ability of it, The tariff and the exchange rate perform a great role to motivate the domestic production and improve the trade balance if we direct them to achieve the same purpose. The Iraqi economic has trade balance which achieve overabundant during the years of studying because of the oil exports, while the other exports represent a small percentage it is hardly remember, and expansion in imports from other side causes exhaustion in oil revenues, and this make the trade balance in permanent
... Show MoreThe resistance of Staphylococcus aureus to ciprofloxacin has complicated the problem of treating staphylococcal associated infections in which MRSA is the causative agent since ciprofloxacin was the drug of choice to treat such infections. Our study investigated the incidence of Ciprofloxacin resistant S. aureus isolates that were also methicillin resistant among Iraqi patients. The obtained bacterial isolates were tested for Ciprofloxacin resistance using agar dilution method and the sequence of gyrA and parC. The results revealed that about 8% of the isolated MRSA strains were Ciprofloxacin resistant and the resistance was due to mutation in gyrA rather than parC.