Objectives The strategies of tissue-engineering led to the development of living cell-based therapies to repair lost or damaged tissues, including periodontal ligament and to construct biohybrid implant. This work aimed to isolate human periodontal ligament stem cells (hPDLSCs) and implant them on fabricated polycaprolactone (PCL) for the regeneration of natural periodontal ligament (PDL) tissues. Methods hPDLSCs were harvested from extracted human premolars, cultured, and expanded to obtain PDL cells. A PDL-specific marker (periostin) was detected using an immunofluorescent assay. Electrospinning was applied to fabricate PCL at three concentrations (13%, 16%, and 20% weight/volume) in two forms, which were examined through field emission scanning electron microscopy (FESEM). The isolated hPDLSCs were implanted on the fabricated PCL. After 21 days, FESEM was conducted to evaluate the implanted scaffolds, and an MTT assay was performed to characterize the biological response of the PCL scaffold at different cell exposure durations (24, 48, and 72 h). Results Periostin was expressed in the expanded PDL cells, and this result revealed that 20% weight/volume PCL scaffold with a pore size of more than 10 μm was the best. The growth rates of PDLSCs were high. Cytotoxicity test of fabricated PCL scaffold demonstrated no significant change in the cell viability when compared with the negative control and no deteriorating or inhibitory effect on growth after different durations. Conclusions A cell sheet was successfully formed by using PCL as a scaffold to cover dental implants and promote PDL cell attachment, proliferation, and growth for biohybrid implant construction.
This work examines the ability of a special type of smart antenna array known as Switched Active Switched Parasitic Antenna (SASPA) to produce a directive and electronically steerable radiation pattern. The SASPA array consists of antenna elements that are switchable between active and parasitic states by using P-Intrinsic-N (PIN) diodes. The active element is the element that is supplied by the radio frequency while short-circuiting the terminals of an element in the array results in a parasitic element. Due to the strong mutual coupling between the elements, a directional radiation pattern with high gain and a small beamwidth can be produced with only one active element operating at a time. By changing the parasitic state to the active
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Hexapod robot is a flexible mechanical robot with six legs. It has the ability to walk over terrain. The hexapod robot look likes the insect so it has the same gaits. These gaits are tripod, wave and ripple gaits. Hexapod robot needs to stay statically stable at all the times during each gait in order not to fall with three or more legs continuously contacts with the ground. The safety static stability walking is called (the stability margin). In this paper, the forward and inverse kinematics are derived for each hexapod’s leg in order to simulate the hexapod robot model walking using MATLAB R2010a for all gaits and the geometry in order to derive the equations of the sub-constraint workspaces for each
... Show MoreObject tracking is one of the most important topics in the fields of image processing and computer vision. Object tracking is the process of finding interesting moving objects and following them from frame to frame. In this research, Active models–based object tracking algorithm is introduced. Active models are curves placed in an image domain and can evolve to segment the object of interest. Adaptive Diffusion Flow Active Model (ADFAM) is one the most famous types of Active Models. It overcomes the drawbacks of all previous versions of the Active Models specially the leakage problem, noise sensitivity, and long narrow hols or concavities. The ADFAM is well known for its very good capabilities in the segmentation process. In this
... Show MoreDrilling fluid loss during drilling operation is undesirable, expensive and potentially hazardous problem.
Nasiriyah oil field is one of the Iraqi oil field that suffer from lost circulation problem. It is known that Dammam, um-Radoma, Tayarat, Shiranish and Hartha are the detecting layers of loss circulation problem. Different type of loss circulation materials (LCMs) ranging from granular, flakes and fibrous were used previously to treat this problem.
This study presents the application of rice as a lost circulation material that used to mitigate and stop the loss problem when partial or total losses occurred.
The experim
... Show MoreThe flow measurements have increased importance in the last decades due to the shortage of water resources resulting from climate changes that request high control of the available water needed for different uses. The classical technique of open channel flow measurement by the integrating-float method was needed for measuring flow in different locations when there were no available modern devices for different reasons, such as the cost of devices. So, the use of classical techniques was taken place to solve the problem. The present study examines the integrating float method and defines the parameters affecting the acceleration of floating spheres in flowing water that was analyzed using experimental measurements. The me
... Show MoreGroupwise non-rigid image alignment is a difficult non-linear optimization problem involving many parameters and often large datasets. Previous methods have explored various metrics and optimization strategies. Good results have been previously achieved with simple metrics, requiring complex optimization, often with many unintuitive parameters that require careful tuning for each dataset. In this chapter, the problem is restructured to use a simpler, iterative optimization algorithm, with very few free parameters. The warps are refined using an iterative Levenberg-Marquardt minimization to the mean, based on updating the locations of a small number of points and incorporating a stiffness constraint. This optimization approach is eff
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