Breast cancer is one of the most important malignant diseases all over the world. The incidence of breast cancer is increasing around the world and it is still the leading cause of cancer mortality An Approximately 1.3 million new cases were diagnosed worldwide last year. With areas rising increasing, risk factors for breast cancer including obesity, early menarche, alcohol and smoking, environmental contamination and reduced or late birth rates become more prevalent. In Iraq, breast cancer ranks first among types of cancers diagnosed in women. This study was conducted on one hundred twenty women with breast cancer that was evaluated and investigated for the possible role of the risk factors on the development of breast cancer in females. The work had been conducted in Baghdad Medical City Hospital. Information on data includes woman's age of breast cancer, marital status, number of complete births, time of lactation, feminine education, menarche age, social activity of women, such as smoking, hormone treatment, and breast cancer history was collected by self-administered questionnaires. Data were collected and analyzed statistically. Differences were significant at (p˂0.05). It is concluded that there is a strong correlation between breast cancer and the risk factors among most of the parameters used. Keywords: Breast cancer, Investigation, marital status, lactation period Correspondence: Natherah Mohammad Ali AL- Nuaimee 1Department of Clinical Laboratory Science, College of Pharmacy, University of Baghdad, Iraq *Corresponding author: Natherah Mohammad Ali AL- Nuaimee emailaddress: [email protected]