Influence of Water Stress on Expression of the X95950 Gene and Several Cellular Callus Components in Violet-Treated Sunflower Varieties

Yusra Ismail Hussein Al-taei; Ibrahim Abdullal Hamza

doi:10.1088/1755-1315/1262/5/052017

Details

Publication Date

Fri Dec 01 2023

Journal Name

Iop Conference Series: Earth And Environmental Science

Volume

1262

DOI

10.1088/1755-1315/1262/5/052017

Choose Citation Style

Statistics

(1)

Influence of Water Stress on Expression of the X95950 Gene and Several Cellular Callus Components in Violet-Treated Sunflower Varieties

Yusra Ismail Hussein Al-taei

Ibrahim Abdullal Hamza

...Show More Authors

AbstractThe research was conducted between 2017 and 2019 at the College of Agricultural Engineering Sciences and Laboratory of Plant Tissue Culture for Postgraduate Studies at the University of Baghdad. One experiment used a totally random design. The experiment examined the effects of PEG (Polyethylene glycol) at concentrations of 0, 2, 4, 6, and 8% on the development of three sunflower types (Ishaqi-1, Aqmar, and AL-Haja) exposed to UV-C rays for 40 minutes as a result of the growing of the juvenile peduncle outside the live body. The aim of the study was to better comprehend the physiological and biochemical changes caused by water stress on the callus of several sunflower varieties. The X95950 gene was amplified using qPCR technology to investigate drought tolerance gene expression and callus cell content of glutathione, ascorbic acid, and APX enzyme as indicators to determine the effect of PEG on callus tissue cells in the nutrient medium. The expression of the X95950 gene was influenced by water stress. It was particularly expressed in Ishaqi-1 at 4 and 8% PEG concentrations, with values of 1.64 and 1.01, respectively. The glutathione content and activity of the enzyme ascorbate peroxidase, which were 56.49 mol-1 and 0.149 mg-1 protein absorption units, differed significantly among the varieties. PEG concentrations significantly influenced glutathione content, with 8% achieving the highest average of 50.07 mol g-1 and 4% achieving the highest averages of ascorbic acid and ascorbate peroxidase enzyme activity of 2.462 mg 100 g-1 and 0.138 mg-1 protein absorption units, respectively. The interaction was considerable, with the maximum average glutathione callus content in the Aqmar variety reaching 69.60 mol g-1 at 6% PEG concentration. At 4% PEG concentration, the Aqmar variety outperformed in terms of APX enzyme efficacy, reaching 0.238 mg-1 absorption units of protein.

View Publication

Publication Date

Wed Jul 05 2017

Journal Name

Chemical Engineering Communications

Microwave-assisted preparation of mesoporous-activated carbon from coconut (Cocos nucifera) leaf by H3PO4activation for methylene blue adsorption

Ali H.

S.

Mohd Azlan Mohd

Lee D.

Siti Solehah

M. K.

...Show More Authors

View Publication

(115)

(107)

Publication Date

Sun Jan 26 2020

Journal Name

Journal Of Global Pharma Technology

Synthesis, Characterization of 2-azido-4-(azido (2-azido-2-( azido carbonyl)-1,3-dioxoian-4-yl)methyl)– 5-((R-azido (hydroxyl) methyl- 1,3-dioxole-2-carbonyl azide. ethanol. hydrate (L-AZD) with Some Metal Complexes

Synthesis

Tri nuclear cluster

Azide

Waleed Khalid Mahdi

Ala H. Hassn

Falih H. Musa

...Show More Authors

The reaction oisolated and characterized by elemental analysis (C,H,N) , 1H-NMR, mass spectra and Fourier transform (Ft-IR). The reaction of the (L-AZD) with: [VO(II), Cr(III), Mn(II), Co(II), Ni(II), Cu(II), Zn(II), Cd(II) and Hg(II)], has been investigated and was isolated as tri nuclear cluster and characterized by: Ft-IR, U. v- Visible, electrical conductivity, magnetic susceptibilities at 25 Co, atomic absorption and molar ratio. Spectroscopic evidence showed that the binding of metal ions were through azide and carbonyl moieties resulting in a six- coordinating metal ions in [Cr (III), Mn (II), Co (II) and Ni (II)]. The Vo (II), Cu (II), Zn (II), Cd (II) and Hg (II) were coordinated through azide group only forming square pyramidal

1 2 3 4 ... 3022 3023 3024 3025