This study has been done for testing the effectiveness and efficiency of probiotic in its ability to inhibit Aspergillus flavus , which secretes the aflatoxin B1 and compare it with materials that were approved to be effective in the inhibition of the growth of fungi and break down and reduction of mycotoxin. these are the filax substance and ozone gas. A laboratory experiment has been conducted to determine the isolates out of the fungus producing the aflatoxin B1.the isolate producing aflatoxin B1 has grown on the yellow corn seed. the probiotic was added along with the filax and they have been exposed to ozone gas and then they were stored from 30 days. A bio test was con conducted to knw the effects of biological toxin on the broiler meat. This study was conducted on 800 broiler chicks (ross 308) , of one day age , the have been weighed on 8 treatment (4 replicate per treatment and 25 chicks per replicate). The first treatment was (T1) which is the control diet that contains yellow corn not contaminated with aflatoxin B1 , the second treatment is (T2) which is the diet that contains yellow corn contaminated with aflatoxin, while the third treatment (T3) it is used the yellow corn which is contaminated with toxin treated with ozone gas. The fourth treatment (T4) it is used the yellow corn which is not contaminated with toxin treated with ozone gas. In the fifth treatment (T5) a toxin contaminated yellow corn was used which is treated with the Iraqi probiotic. The sixth treatment (T6) the yellow corn not contaminated with toxin and treated with Iraqi probiotic was used. The seventh treatment (T7) the yellow corn was contaminated with the aflatoxin and treated with filax and the eighth treatment (T8) the yellow corn not contaminated with toxin was used but it was treated with filax. The results of the study indicated that the addition of ozone gas, the filax and the probiotic to the yellow corn contaminated with toxin led to a significant reduction in toxin amount in comparison with control treatment, which were contaminated with toxin without adding any other substances to them. A significant higher preponderance (P<0.05) and a clear improvement in productive performance of the birds that were fed with diets containing yellow corn and treated with the probiotic. Such improvement was clear also in the treatment to which the ozone gas and the filax were added. The study showed a significant deterioration (p<0.05) in productive characteristics of the birds in which yellow corn, contaminated with aflatoxin B1 was used from this study we conclude there is possibility to add the Iraqi probiotic to the diets of broilers meat that contain aflatoxin B1 to reduce the contamination of diets with toxin, in addition to the efficiency and obvious effectiveness of the filax and ozone gas in eliminating and destroying the toxin.