Background and Objectives: Urinary tract infections (UTIs), among a wide range of microbial infections, are of a double-edged worry with health-care and economic implications. They are serious diseases that can influence various parts of the urinary tract. The aim of this study was characterization of the enteric bacteria isolated from urine of human UTIs and studying their antimicrobial sensitivity. Materials and methods: A total of 50 urine samples were collected from patients with UTIs of both genders. The isolates identification was done using routine diagnostic methods and confirmed by Vitek2. Antimicrobial susceptibility was done against 10 antimicrobials. Results: Both genders of human were found to suffer from urinary tract problems caused by bacteria. Out of 50 patients, 45 (90%) of the cases showed bacterial growth. Approximately, 30.43% of the human infections were found to be caused by members of the Enterobacteriaceae family. The ratio of female patients with UTIs was more than that of males, the most common bacterium isolated from human urine was E. coli, which constituted approximately 85.7% of the enteric bacteria isolated and 26.1% of all bacterial isolates. Other members of Enterobacteriaceae family were also isolated from patients enrolled in this study, such as Citrobacter freundii, which constituted the same incidence rate as K. pneumoniae. Concerning antimicrobial resistance, 11, 10, 9, and 8 out of 12 of E. coli isolates were resistant to Erythromycin, Vancomycin, Tetracycline, and Ceftazidime together, respectively, with a range of resistance from 91.7% to 66.7%. Low percentages of bacteria showed intermediate sensitivity to Imipenem, Gentamicin, Chloramphenicol, Vancomycin, and Erythromycin. However, 12, 11, 10, 10, 9, and 8 out of 12 isolates were susceptible (susceptibility ranged from 100% to 66.7%) to each of Cefotaxime, Chloramphenicol, Imipenem, Amikacin, Ciprofloxacin, and Gentamicin. Conclusions: Escherichia coli was the most common bacteria isolated from human UTIs. All of the isolates were multi-drug resistant toward at least four antimicrobials. Particularly, Erythromycin and Vancomycin had no effect on the enteric bacteria at all. Imipenem might be the most effective drug against a large number of the human isolates.
This study aimed at isolating uropathogenic Escherichia coli from urinary tract infections (UTIs) of human and cattle to examine the molecular diversity and phylogenetic relationship of the isolates. A total of 100 urine samples were collected from UTIs of human and cattle. The isolates identification was done using routine diagnostic methods and confirmed by Vitek2. Antimicrobial susceptibility was tested against 10 antimicrobials. Random amplified polymorphic DNA (RAPD)-polymerase chain reaction (PCR) was applied to identify the genetic diversity among E. coli isolates from human and animal origin by using five different octamer primers. The gelJ software for the phylogenetic analysis created Dendrograms. Out of 50 human urine samples, E.
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Ten isolates of Klebsiella pneumoniae, seven isolates of Pseudomonas aeruginosa and nine isolates of Staphylococcus aureus, were obtained from 100 urine samples collected from Baghdad hospitals. All isolates were identified biochemically and confirmed by using VITEK 2 and were then tested for their susceptibility towards 6 antibiotics and for phenolic extracts of Thymus vulgaris and Cinnamomum cassia. All bacteria were greatly affected by T. vulgaris, especially K. pneumoniae. Viable count was performed, it was noted that the number of bacterial cells reduced from 1×108 CFU to 1.2× 103, 2×105 and 1.8×106CFU of K. pneumoniae, P. aeruginosa and S. aureus respectively. While C. cassiahad a slight effect on them. K. pneumoniae isola
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Objectives: The objectives of the present study are to identify both, the rate of urinary tract infections (UTI) in medical
and surgical wards in Baghdad Teaching Hospital and the common types of microorganisms that cause this type of NIs,
study the resistance of isolated microorganisms to several types of antibiotic and the effect of some disinfection
material like Iodine, chlorhexidine and septicin on the growth of these isolates.
Methodology: This is descripƟve study carried out from 1\6\2009 to 1\11\2009. A total of 200 paƟents were admiƩed
to medical and surgical wards in Baghdad teaching hospital, paƟents were divided into two group 100 paƟents, in
surgical ward and 100 paƟents in medical ward. Some of impor
Antibiotic resistance is a problem of deep scientific concern both in hospital and community settings. Rapid detection in clinical laboratories is essential for the judicious recognition of antimicrobial resistant organisms. So, the growth of Uropathgenic Escherichia coli (UPEC) isolates with Multidrug-resistant (MDR) and Extensively Drug-resistant (XDR) profiles that thwart therapy for (UTIs) has been detected and has straight squeezed costs and extended hospital stays. This study aims to detect MDR- and XDR-UPEC isolates. Out of 42 UPEC clinical isolates were composed from UTI patients. The bacterial strains were recognized by standard laboratory protocols. Susceptibility to antibiotic was measured by the standard disk diffusi
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Urinary tract infection is a bacterial infection that often affects the bladder and thus the urinary system. E. coli is one of the leading uropathogenic bacteria that cause urinary tract infections. Uropathogenic E. coli is highly effective and successful in causing urinary tract infections through biofilm formation and urothelial cell invasion mechanisms. Other organisms that cause urinary tract infections include members of the Enterobacteriaceae family, streptococci and staphylococci species and perch. In addition, K.penumoniae is another important gram-negative bacterium that causes urinary tract infections. With the PCR technique, unseen bacterial species can be detected using standard clinical microbiology methods. In this study, the
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Background: The emergence and spread of multidrug-resistant Gram-negative bacilliin burn wound infections related to biofilm formation, which lend to challenge in treatment with conventional antibiotics andprompting to search for novel antimicrobial agents to control the infections.Silver nanoparticles (AgNPs) have wide spectrum biological properties with different mechanisms of action and less toxicity towards human cells.
Objective:The goal of this study was to evaluated the anti-bacterial and anti-biofilm activities of AgNPs alone and in combination with aminoglycoside (Amikacin) and β-lactam (Ampicillin) antibiotics against multidrug resistant Gram-negative bacilli (Pseudomonas aeruginos
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An increasing trend to use probiotic and study their effects on the pathogens has been conductor where they are defined as live micro-organisms that give a health benefit to the host when ingested in sufficient quantities, including the yeast Saccharomyces boulardii In addition research show that a magnetic field (MF) has a biological effect. This study aims to investigate the effects of magnetic field on the inhibitory action of Saccharomyces boulardii against bacteria isolated from urinary tract infection, Study the sensitivity of bacterial isolates to antibiotics after diagnosis by microscopic, Cultural and biochemical examinations as well as Api20 E examinations were used gram negative bacteria , Most isolates were resistant to an
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