Background: The present work investigated the profile and biodiversity of the pathogenic streptococci species isolated from local and imported mozzarella soft cheese in Baghdad City from October 2022 to January 2023. The study aimed to examine the molecular characterization of 16Sr RNA gene in some streptococcus species isolates from mozzarella soft cheese in Baghdad city Methods: From 50 samples, 8 isolates in all were found and identified based on the VITEK, molecular, and sequencing of the 16SrRNA gene. The eight isolates represented Lactococcus cremoris. Streptococcus alactolyticus, Streptococcus sanguinis, and Streptococcus thoraltensis. The isolates were subjected to conventional PCR and electrophoresis to detect the 16SrRNA gene using specific primers , Streptococcal genes were used in the neighbor-joining phylogenetic analysis that determined these sequences were derived from Streptococcal genes. Results: DNA sequencing evaluation Significant alignments (96-99% identities) to the Streptococci isolates found in BLAST-NCBI Gene-bank were discovered after sequencing the bacterial DNA products generated by PCR. The results revealed that 8/50 amplified the 16SrRNA gene, which has a molecular weight of about 1250 bp. the gene distribution was statistically significant (p ≤ 0.05). Conclusion: Streptococcus isolates from mozzarella soft cheese by using molecular tools to first stop the spread of the epidemic of harmful microorganisms in food, it is represented a health risk for the consumers.
The aim of this study is to determine the level of pollution with heavy metals (Cd, Cr, Cu, Ni, Pb, Zn) and their potential sources in dust samples collected from schools in Ramadi City, Iraq. The dust samples were collected from 40 primary schools and two kindergartens and analyzed by using atomic absorption spectrophotometer. The heavy metal concentrations were found to follow the order Cr > Cu > Pb > Ni > Zn > Cd. The results indicated that the concentrations of Cd, Cu, and Pb exceeded the permitted background values. The pollution level was assessed using the geo-accumulation index (Igeo) and pollution load index (PLI). The classification of dust samples according to Igeo values showed that
... Show MoreOne of the most significant elements influencing weather, climate, and the environment is vegetation cover. Normalized Difference Vegetation Index (NDVI) and Normalized Difference Built-up Index (NDBI) over the years 2019–2022 were estimated based on four Landsat 8 TIRS’s images covering Duhok City. Using the radiative transfer model, the city's land surface temperature (LST) during the next four years was calculated. The aim of this study is to compute the temperature at the land's surface (LST) from the years 2019-2022 and understand the link, between LST, NDVI, and NDBI and the capability for mapping by LANDSAT-8 TIRS's. The findings revealed that the NDBI and the NDVI had the strongest correlation with the
... Show MoreThe current study included bioremoval of chromium metal ions from aqueous solution by using seventeen Pseudomonas aeruginosa species isolated from different environments. The experimental results showed that isolates Pseudomonas aeruginosa have high efficiency in removal of chromium where the P. aeruginosa p.8 was the most efficient (P≥0.001) in bioremoval of chromium with a removal capacity reached 92.5 mg/L and removal index reached (96.5%). While P. aeruginosa p.4 was the least efficient (P≥0.001) in bioremoval of chromium from aqueous solutions reached 74.6 mg/L and removal index reached (79.8%). The REP-PCR detection using BOX-primer, showed genetic relatedness among the isolates of P.aeru
... Show MoreIn recent years, there is more interest in water sources availability, including groundwater due to an increase in demand for water because of the increasing population in the world, and the water recedes due to climate change also. Therefore, the study of groundwater has required more attention. The aim of the present study is to establish a MODFLOW model in the groundwater modeling system software to simulate the movement of groundwater in the Turssaq alluvial fan which is located in the Qazaniyah city, east of Diyala Governorate. The solid model was used to define the aquifer in the study area. Using the GIS software, mapping and preparing the data needed to create a conceptual model were carried out. The data of the
... Show MoreThe aim of this research was to indicate the opinion of the Iraqi consumer about the quality and safety of local food products, the questionnaire was included 19 questions for product quality, price, distribution and promotion as a tool to survey the opinions of 128 consumers in Baghdad, the data was analyzed by using percentage, weighted mean, and weight percent, the results obtained showed that the Iraqi consumer prefer local food products for their high quality and appropriate price, however they need attention to packaging, promotion and distribution.
Trace elements concentrations are frequently reported to be a good indicator for diagnosis and prognosis of some disease. The levels of copper, zinc, manganese, and magnesium were measured in serum of two group. Group one contains (52) newborn jaundice patients while the second group contains (20) healthy newborn used as controls.
In this study the concentration of Cu, Zn, Mg, and Mn were measured using atomic absorption spectrophotometer (AAS). It has been found that zinc level is significantly lower in newborn jaundice patients compared with normal subjects at (p<0.05) , also the level of Cu, Mg, and Mn were significantly
... Show MoreA quantitative interpretation of gravity and magnetic anomalies in west of Tikrit city and surroundings, has been completed utilizing Grav2dc and Mag2dc (2D, 2.5D) forward techniques. The modeling has been carried out along four profiles, two NW-SE profiles along the distinct gravity residual anomalies and two NE-SW profiles along the magnetic residual anomalies. The most geologic plausible model that matches the data was picked. The model along the gravity profile (A-A') reveal faulting of the basement, whereas along the profiles B-B', C-C' and D-D' did not present faulting. The models comprise of two rock units, the first is the sedimentary cover and the second unit i
... Show MoreMilling process is a common machining operation that is used in the manufacturing of complex surfaces. Machining-induced residual stresses (RS) have a great impact on the performance of machined components and the surface quality in face milling operations with parameter cutting. The properties of engineering material as well as structural components, specifically fatigue life, deformation, impact resistance, corrosion resistance, and brittle fracture, can all be significantly influenced by residual stresses. Accordingly, controlling the distribution of residual stresses is indeed important to protect the piece and avoid failure. Most of the previous works inspected the material properties, tool parameters, or cutting parameters, bu
... Show MoreObjective(s): The present study describes the quality of life (QOL) among adolescents with age of (12-22) year old and identifies factors that may be associated with it. Methodology: A cross-sectional design is carried throughout to evaluate adolescents' quality of life in Hilla City for the period of September 2nd 2016 to November 6th 2016. The present study is conducted in different settings that include schools and colleges in Hilla City. A non-probability "purposive" sample of (144) participant is selected and comprised of (50%) males and (50%) females. An instrument is constructed for the intent of the st