Preferred Language
Articles
/
6xfCX5MBVTCNdQwCS9Id
CLINICAL UTILITY OF URINARY ANTIGEN TEST AND MOLECULAR METHOD FOR DETECTION OF LEGIONELLA PNEUMOPHILA

Background: Legionella pneumophila (L. pneumophila) is gram-negative bacterium, which causes Legionnaires’ disease as well as Pontiac fever. Objective: To determine the frequency of Legionella pneumophila in pneumonic patients, to determine the clinical utility of diagnosing Legionella pneumonia by urinary antigen testing (LPUAT) in terms of sensitivity and specificity, to compares the results obtained from patients by urinary antigen test with q Real Time PCR (RT PCR) using serum samples and to determine the frequency of serogroup 1 and other serogroups of L. pneumophila. Methods: A total of 100 pneumonic patients (community acquired pneumonia) were enrolled in this study during a period between October 2016 to April 2017; 92 samples were collected from patients attended and admitted to Al-Imamein Al-Kadhimein Medical City and 8 samples from those in the (Center of Kidney Diseases and Transplantation) in the Medical City of Baghdad. All patients were under therapy with antibiotics. Serum and urine specimens were obtained from all patients; urine samples were processed for urinary antigen test (rapid test). Serum samples were collected and submitted to DNA extraction for detection of L. pneumophila mip gene by q RT PCR assay. Results: The percentage of L. pneumophila in two hospitals in Bagdad was 30%. Of these 26% was serogroup 1 detected by urinary antigen testing (UAT). In the other hand, 23% of samples were positive by q RT PCR based mip gene, of these 19 % were serogroup 1 and 4% were another serogroup. The sensitivity of UAT is high (P value < 0.001), which means statistically highly significance than q RT PCR. Conclusion: LPUAT is a rapid tool for early diagnosis of Legionella infection, which highlights the need of using this test in hospitals and health institutions and there is a high prevalence of L. pneumophila in Iraq that refer to the necessity of considering this microorganism point of view in future studies for detection and treatment in pneumonic patients. Keywords: L. pneumophila, mip gene, quantitative real time PCR, urinary antigen. Citation: Gauad SA, Abdulrahman TR, Muhamad AK, Jawad AA, Hassan JS. Clinical utility of urinary antigen test and molecular method for detection of Legionella pneumophila. Iraqi JMS. 2018; 16(2): 207-215. doi: 10.22578/IJMS.16.2.13

Crossref
View Publication Preview PDF
Quick Preview PDF
Publication Date
Sun Jan 04 2015
Journal Name
Journal Of The Faculty Of Medicine Baghdad
Detection of JAK2V617F tyrosine kinase mutation and estimation of serum erythropoietin in blood donors who have high hematocrit

Background: JAK2V617F mutation is the most prevalent molecular abnormality in myeloproliferative neoplasms (MPNs) and has become a valuable marker for diagnosis of MPNs. Almost all patients with polycythemia vera (PV) have this acquired mutation. However, it has also been found in many other hematological diseases, and some studies even detected the presence of JAK2V617F in normal blood samples.
Objectives: To discuss the actual need to defer blood donors with high hematocrit.
Patients and methods: This prospective case control study was started on 16th of December 2012 and completed on 8th of July 2013, and enrolled 94 male blood donors who attended the National Blood Transfusion Center (NBTC) in Bagh

... Show More
Crossref
View Publication Preview PDF
Publication Date
Tue Jun 30 2009
Journal Name
Al-kindy College Medical Journal
Abdominal Distention and Acute Urinary Retention secondary to Congenital Distal Vaginal Obstruction in a Newborn Female

Congenital distal vaginal obstruction is usually asymptomatic in a newborn female. On rare occasions, it may present as an acute emergency with life threatening complications.This paper is reporting the rare condition of two newborn females presenting urgently with abdominal distension and acute urinary retention as a result of congenital distal vaginal Obstruction. The case history and urgent management shall be presented and both conditions shall be discussed.

View Publication Preview PDF
Publication Date
Mon Apr 01 2013
Journal Name
مجلة كلية بغداد للعلوم الاقتصادية الجامعة العدد الخاص بمؤتمر الكلية
A propose method for hiding image into image

Publication Date
Fri Jan 26 2024
Journal Name
Iraqi Journal Of Science
A simple Cascade Method for Mixed Noise Removal

Images are usually corrupted by type of noise called "mixed noise ", traditional
methods do not give good results with the mixed noise (impulse with Gaussian
noise) .In this paper a Simple Cascade Method (SCM) will be applied for mixed
noise removal (Gaussian plus impulse noise) and compare it's performance with
results that acquired when using the alpha trimmed mean filter and wavelet in
separately. The performances are evaluated in terms of Mean Squane Error (MSE)
and Peak Signal to Noise Ratio (PSNR).

View Publication Preview PDF
Publication Date
Thu May 01 1997
Journal Name
Polymer-plastics Technology And Engineering
An Efficient Method for Real Gas Pseudopressure Calculation

Scopus (2)
Crossref (1)
Scopus Clarivate Crossref
View Publication
Publication Date
Sun Oct 01 2017
Journal Name
Journal Of The Association Of Arab Universities For Basic And Applied Sciences
Semi-analytical method for solving Fokker-Planck’s equations

Crossref (6)
Crossref
View Publication
Publication Date
Tue Feb 28 2023
Journal Name
Iraqi Journal Of Science
Modified Iterative Method for Solving Sine - Gordon Equations

       The basic goal of this research is to utilize an analytical method which is called the Modified Iterative Method in order to gain an approximate analytic solution to the Sine-Gordon equation. The suggested method is the amalgamation of the iterative method and a well-known technique, namely the Adomian decomposition method. A method minimizes the computational size, averts round-off errors, transformation and linearization, or takes some restrictive assumptions. Several examples are chosen to show the importance and effectiveness of the proposed method. In addition, a modified iterative method gives faster and easier solutions than other methods. These solutions are accurate and in agreement with the series

... Show More
Scopus Crossref
View Publication Preview PDF
Publication Date
Thu Jul 20 2023
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Direct Method for Variational Problems Using Boubaker Wavelets

The wavelets have many applications in engineering and the sciences, especially mathematics. Recently, in 2021, the wavelet Boubaker (WB) polynomials were used for the first time to study their properties and applications in detail. They were also utilized for solving the Lane-Emden equation. The aim of this paper is to show the truncated Wavelet Boubaker polynomials for solving variation problems. In this research, the direct method using wavelets Boubaker was presented for solving variational problems. The method reduces the problem into a set of linear algebraic equations. The fundamental idea of this method for solving variation problems is to convert the problem of a function into one that involves a finite number of variables. Diff

... Show More
Crossref
View Publication Preview PDF
Publication Date
Wed Aug 01 2018
Journal Name
Engineering And Technology Journal
A Proposed Method for the Sound Recognition Process

Crossref
View Publication
Publication Date
Wed Jan 01 2014
Journal Name
Journal Of Next Generation Information Technology
Face detection and the effect of contrast and brightness

We propose a system to detect human faces in color images type BMP by using two methods RGB and YCbCr to determine which is the best one to be used, also determine the effect of applying Low pass filter, Contrast and Brightness on the image. In face detection we try to find the forehead from the binary image by scanning of the image that starts in the middle of the image then precedes by finding the continuous white pixel after continuous black pixel and the maximum width of the white pixel by scanning left and right vertically(sampled w) if the new width is half the previous one the scanning stops.

Scopus (1)
Scopus