درست عملية تحييد البلازميد لسبع عزلات محلية لبكتريا pneumoniae Klebsiella المتعددة المقاومة للمضادات الحيوية والمعزولة من حالات التهابات المجاري البولية باستخدام مادتي الـ Acridin .(SDS) Sodium dodecyl sulphate والـ orange أظهرت النتائج ان العزلات المحلية لجرثومة pneumoniae.K كانت ذات مقاومة عالية جدا ًاذ بلغت (%100) لمضادات امبيسيلين،ببراسيلين، اموكسيسيلين، بنسلين ج، تتراسايكلين، ارثرومايسين وجنتامايسين واظهرت مقاومة عالية (71–%86) لمضادات سيفاليكسين، سيفالوثين، سيفتازديم، سبروفلوكساسين، ستربتومايسين والتوبرامايسين، ومقاومة متوسطة (43–%57) لمضادات سيفاكلور، سيفرادين، ونالدكسك اسيد، في حين انها اظهرت مقاومة ضعيفة (%29) لمضاد سيفوتاكسيم وانها كانت حساسة لمضادات كلورامفينيكول واميكاسين. ومن خلال عملية تحييد البلازميد وجد ان مادة الـ SDS اكثر كفاءة في احداث التحييد مقارنة بالـ Acridine orange حيث لوحظ فقدان المقاومة للمضادات الحيوية بالنسبة للـ SDS بمدى (70– %100) اما بالنسبة للـAcridine فكانت (28–%100)، وبعد عملية التحييد فقدت المقاومة لمضادات الببراسيلين، اموكسيسيلين، بنسلين ج، سيفاليكسين، سيفالوثين،سيفتازديم، تتراسايكلين، جنتامايسين وتوبرامايسين مما يدل على ان صفة مقاومة هذه المضادات الحيوية محمولة على البلازميد في حين لم تفقد مقاومة مضادات الامبيسيلين، سبروفلوكساسين، ارثرومايسين وستربتومايسين مما يدل على ان المورثات التي تشفر لمقاومتها محمولة على الكروموسوم.
Klebsiella pneumoniae are Gram-negative which cause many diseases such as urinary tract infections, respiratory tract infections and septicemia. Inulinase is an enzyme used in food manufacture and pharmaceuticals. Inulinase is used in decreasing lipid ratio and, cholesterol in blood and considered as a prebiotic factor inside intestine. Many microorganisms can produce inulinase, such as yeast, fungi and bacteria; among such bacteria: Bacillus spp., Arthrobacter spp., and Pseudomonas spp. but there are no studies about inulinase production by K. pneumoniae have been reported. So the current study aims at investing the ability of producing and purification inulinase by K. pneumoniae. Method: K. pneumoniae were isolated from many hospitals and
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پس از اعلام جنبش مشروطه , نویسندگان ایرانی فرصت مناسب به مبارزه آزاد و تعبیر واضح از قلم یافتند . بیشتر مجلات و روزنامه ها و ده ها تن از أنها را گسترش در شهرهای ایرن یافتند , اما نثر روزنامه نگاری به تنهایی نمی تواند ارضای میل آزادی و واعظان کند برای ابراز به برجسته احساسات سیاسی واجتماعی , وبه همین دلیل است به انواع دیگر از ن
... Show MoreA total of 47 samples were collected from different clinical specimens (urine, wounds, burns, sputum, blood, stools) during the period from November 2013 to
January 2014, only 18 isolates (38.29%) were identified as Klebsiella pneumoniae ,11isolates (23.4%) as E. coli , 9 isolates (19.1%) as S. aureus, 3isolates (6.3%) as Psedomonase spp., 2 isolates (4.2 %) as K. terrigena and 4 isolates (8.5%) as K. oxytoca. The results were shown the elevation of K. pneumoniae percentage among the bacterial isolates depending on cultural, microscopically, biochemically characteristics and confirmed by using the API 20E and VITEK 2 system . Also K. pneumoniae isolates were more frequently in sputum samples then burns, stools , urine, wounds ,and blo
Out of 150 clinical samples, 50 isolates of Klebsiella pneumoniae were identified according to morphological and biochemical properties. These isolates were collected from different clinical samples, including 15 (30%) urine, 12 (24%) blood, 9 (18%) sputum, 9 (18%) wound, and 5 (10%) burn. The minimum inhibitory concentrations (MICs) assay revealed that 25 (50%) of isolates were resistant to gentamicin (≥16µg/ml), 22 (44%) of isolates were resistant to amikacin (≥64 µg/ml), 21 (42%) of isolates were resistant to ertapenem (≥8 µg/ml), 18 (36%) of isolates were resistant to imipenem (4- ≥16µg/ml), 43 (86%) of isolates were resistant to ceftriaxone (4- ≥64 µg/ml), 42 (84%) of isolates were resistant to ceftazidime (1
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The organizations recognize what the effective role of time management in achieving competitive advantage and success. But the effectiveness depends on integrating some organization actions which perspective are relating with it by significance relations and high affect. From this the research problem is arising، which descripe the recognizing of libraries managers for the role of some organizational actions. The samples from (40) libraries managers are chosen.
The major results: these organizational actions are related and affected on the time management if integrated with one anothers more than if related in separate. &nbs
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In this paper, quantified study of the biofilm formed by Klebsiella pneumoniae isolated from urine specimen of patient suffering from acute urinary tract infection (UTI) on catheter, stainless-steel and glass coupon surfaces, as well as determine the relationship between time contact and biofilm progression using crystal-violet binding assay based on the values of optical density at 620nm of the crystal violet stain which bonded total biofilm biomass by resolubizing with 99.9% ethanol at the specific interval times. The result showed biofilm formed on three tested surfaces but in different degrees. According to obtained data, the catheter coupons presents a higher capability to attract bacteria cell and biofilm formation followed by glas
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Quorum sensing (QS) is a perfectly orchestrated molecular communication system. It is a boon for Klebsiella pneumoniae, and bane for the host. This system is believed to make K. pneumoniae a leading cause of multidrug-resistant (MDR) nosocomial infections. This study aimed to investigate the antibacterial and anti-biofilm potential of medicinal plant extracts through interfering with QS of K. pneumoniae. The effect of different concentrations of ethanolic extracts of cinnamon and clove on K. pneumoniae was determined by analyzing the growth curve, survival assay (MTT), Qualitative and quantitative biofilm formation, antibiotic resistance, along with studying gene expression of the genes encoding the above traits, using quantitative real tim
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