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Role of plant growth regulators in gene expression of SGR gene responsible for stay green of wheat varieties
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A field experiment was carried out during winter season of 2019-2020 at Al-Mhanawyah Research Station - Agriculture Research Directorate - Babylon Governorate / Iraqi, to study the gene expression of Sgr gene responsible for controlling the duration of staying green in varieties of wheat under effect of plant growth regulator during the two growth stages (vegetative and reproductive) by using quantitative reverse transcription-PCR (RT-qPCR) technique and achieving the highest grain yield for a number of wheat varieties. Randomized complete block design (RCBD) arranged according to split plots used with three replicates. The experiment included twelve wheat varieties (Saberbic, Al-Rasheed, Iraq, Tamoz-3, Al-Adnaniya, Babel, IPA-99, Al-Latifeya, Al-Hashemia, Al-Naeema, Buhooth-10 and Al-Baraka) within main plots and nine treatments of plant growth regulator spraying (spraying of distilled water at tillering and anthesis stages, spraying of Abscisic acid (ABA) at tillering stage, spraying of ABA at anthesis stage, spraying of ABA acid at tillering and anthesis stages, spraying of kinetin at tillering stage, spraying of kinetin at anthesis stage, spraying of kinetin at tillering and anthesis stages, spraying of ABA at tillering stage + kinetin at anthesis stage and spraying of ABA at anthesis stage + spraying of kinetin at tillering stage) within sub plots. The results showed that ABA spraying caused a significant increase in the gene expression of Sgr gene, whereas the spraying of kinetin caused a significant decrease in gene expression. It was observed that the Saberbic variety gave the highest gene expression of Sgr gene (1.44). Al-Rasheed and Iraq varieties showed that the decrease in the vegetative growth period which accounted 65% of lives cycle compared with reproductive stage (35%). Also, It is observed that the spraying of ABA was decreased the number of days of the stage in which it is sprayed or after, while the spraying of kinetin was increased the number of days of the stage in which it is sprayed or after. Besides, the spraying of kinetin at tillering stage revealed a significant increase in the biological yield (11.68-ton ha-1), whereas the spraying of ABA at tillering stage + kinetin at anthesis stage had the highest mean of grain yield of (4.63-ton ha-1). Al-Rasheed variety gave the highest mean of grain yield (5.56-ton ha-1).

Scopus
Publication Date
Thu Jul 20 2023
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Role of Ascorbic Acid in Drought Stress Tolerance of Wheat Plant Triticum aestivum L.
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Drought is a major abiotic stress, severely affecting the physiological processes and  growth of plants. The present investigation aimed to evaluate the impacts of drought stress with three periods for irrigation: every 3 days(control), every 6 days, and every 12 days and foliar spray with different concentrations of ascorbic acid (0, 75, 150, 225) mg.L-1  on the wheat plant. Pots experiment was conducted as a completely randomized design with three replications in the green house, Department of Biology, College of Education for Pure Science (Ibn Al- Haitham), University of Baghdad, during the growing season, 2018 – 2019. The results indicated that drought stress decreased plant growth and hastened the senescence of flag l

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Publication Date
Sun Oct 31 2021
Journal Name
Al-rafidain Journal Of Medical Sciences ( Issn: 2789-3219 )
Evaluation of EOS Gene Expression and IL-6 Serum Levels in Iraqi Patients with Psoriasis
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Background: EOS (encoded by the IKZF4 gene) is a member of the zinc finger transcription factor IKaros family, and plays a critical role in Treg suppressor functions, and maintaining Treg stability. IL-6 is a soluble mediator with a pleiotropic effect on inflammation, immune response, and hematopoiesis. Aim: To estimate serum IL-6 level and EOS gene expression in Iraqi patients with psoriasis. Method: Twenty-two patients with psoriasis (8 females, 14 males) with age ranged 18-72 years, were recruited from Baghdad Teaching Hospital, Dermatology Clinic, Baghdad, and 24 healthy donors. The serum levels of IL-6 by ELISA and the gene expression of IKZF4 (EOS gene) by RT-qPCR technique. Results: The results showed a non-significant diffe

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Publication Date
Thu Nov 30 2023
Journal Name
Iraqi Journal Of Science
The Correlation Study between TP53 Gene Expression and Acute Myeloid Leukemia in Iraq
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     Acute myeloid leukemia represents the most prevalent type of acute leukemia in adults. Mutations in the tumor protein (TP53) gene have been found in more than half of all human cancers. This study was done to investigate the relationship between TP53 gene expression and the appearance and progression of acute myeloid leukemia in Iraq. This study included 100 subjects, divided into 60 patients suffering from pre-diagnostic acute myeloid leukemia and 40 healthy individuals. The difference in TP53 gene expression between acute myeloid leukemia patients and healthy individuals has been investigated, and the gene expression of TP53 has been measured after extraction of total RNA at concentrations (15–83 n

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Publication Date
Tue Aug 01 2023
Journal Name
Biochemical And Cellular Archives
Interleukin-32(IL-32) gene expression in Iraqi chronic hepatitis B virus patients
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Hepatitis B infection is a prominent infectious disease caused by hepatitis B virus (HBV), which infect liver and is considered as the main cause of liver cirrhosis, fibrosis and liver cancer worldwide. A pro-inflammatory cytokine Interleukin32 is believed to have a role in chronic HBV infections. Since its role in CHB infections is remain unclear, this study was done to detect IL-32 gene expression in CHB patients in order to identify its exact role. A total number of 110 blood samples were collected from Gastroenterology and Hepatology Teaching Hospital in Baghdad Medical City from CHB patients for both males and females with different age groups according to the research ethics form then sent to Central Public Health Laboratory (CPHL),

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Crossref
Publication Date
Sat Sep 30 2023
Journal Name
Iraqi Journal Of Science
Association of SCARB1 Gene Expression with Chronic Myeloid Leukemia Progression in a Sample of Iraqi Patients
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     The present research design examines the relationship between SCARB1 gene expression and the progression of chronic myeloid leukemia (CML) in Iraqi patients. The variations in gene expression between patients with CML and healthy controls were investigated. The gender and age correlations with CML patients were included, as was the association of gene expression folding of the SCARB1 gene with clinical data (WBC, RBC, hemoglobin, platelets, and BCR-ABL gene). The results displayed a significant difference in the mean gene expression level (∆Ct) of the CML group when compared to the matching ∆Ct values in the healthy control group. The gene expression folding of the SCARB1 gene indicates considerable changes in expression, wh

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Publication Date
Fri Jun 24 2022
Journal Name
Iraqi Journal Of Science
Effect of electric and heat shock on morphological characteristics for two varieties of wheat
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Wheat (Triticum aestivum L.) is one of the Poaceae family (Gramineae). Is momentous for human nutrition, and the stresses can affect strongly on the phenotype characteristics of the plant. The aim of this study was to determine how electric shock on germinated grain (for 2.5, 5, and 7.5 mins.) and heat shock at ( 35 oC, 40 oC and 45 oC) applied after phase out the radical length of 2-5 mm, from the grain of two wheat Varieties: “Baghdad 1” and “Babylon 113”. The electric shock for 2.5 mins., lead to delay of flowering, from day to 50 % flowering was 93 day, as well as, gave lowest value of plant height 64.5cm and lowest spike length was 10.7 cm. While The highest flag leaf area was obtained by electric shock for 5 mins. was 56.6

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Publication Date
Wed Jan 01 2020
Journal Name
Sylwan Journal
Histological Evaluation and Gene Expression of Local Application of Calmodulin on Wound Healing of rats
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Background and Objectives: Wound healing is a complex process with overlapping phases haemostasis, inflammation, proliferation and maturation/matrix remodeling. Each phase of wound healing requires different management strategies, and inappropriate treatment can delay wound healing. The aim of the present study was to evaluate the efficacy of topical application of calmodulin as a significant augmentation of the granulation tissue production process of wound healing and to express of genes CaMKK2, MaP2K6 and CXCR4 at site of wound defect, that have versatile effects on the body and they belong to Ca/camodulin related genes. Material and Methods: In this study thirty albino male rats, weighting (300-400) gram, aged (6-8) months, wil

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Publication Date
Thu Oct 01 2020
Journal Name
Biochemical & Cellular Archives
THE STUDY ON ABILITY OF ESCHERICHIA COLI ISOLATED FROM DIFFERENT CLINICAL CASES TO BIOFILM FORMATION AND DETECTION OF CSGD GENE RESPONSIBLE FOR PRODUCE CURLI (FIMBRIAE)
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A total of 165 clinical sample included Urine, Swab wounds and Burns were collected from Baghdad Governorate. Results showed that rate all isolates of E. coli was 50(30.3%) and rate of urine infection was 46(92%) and rate of swab wounds infection 4(8%). Where was diagnostic based on streaked on MacConkey agar, then single colony was transferred to Eosin Methylene Blue (EMB). Identification some of the biochemical test included: Catalase test, Oxidase test, Indole test, Methyl red, Vogues - Proskauer test and Citrate Utilization test. Then confirmed by the Vitek - 2 Compact System. The ability of E.coli isolate to biofilm formation to be studied it is considered one of the most important factors of virulence and has role in causing injury an

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Scopus
Publication Date
Mon Jan 30 2023
Journal Name
Iraqi Journal Of Science
Gene Expression Evaluation of Intracellular Adhesins and Regulatory Genes among Biofilm Producing MRSA Isolates
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       The gene expression of the most important structural genes ica A and D of biofilm, sarA, and sigB regulatory genes of some methicillin-resistant Staphylococcus aureus (MRSA) isolates were examined using the real-time polymerase chain reaction after 24 hours of growth. The results revealed that the isolates with strong biofilm production had the highest gene expression of the structural icaA and D genes. Whereas the isolates that showed moderate and weak biofilm production, recorded the lowest gene expression. The results of the regulatory genes sarA, and sigB fluctuated among all MRSA isolates. Isolate No. 64 recorded the highest gene expression

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Publication Date
Sun May 28 2017
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Performance Evaluation of three Indian Wheat Varieties Under Iraqi Environment
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 The performance of the Indian wheat varieties (PBW34, PBW343 and WH542) was compared with the local varieties (Tamose – 2 and Abu Ghareeb).      The experiment was conducted during two seasons first 2000-2001 and second 2001-2002 at AL- Twaitha experimental station , middle of Iraq.         The results showed that (Tamose – 2) exceeded the other varieties in plant height and heading date in the two seasons, while WH542 gave the lowest plant height.  PBW34 variety showed a significant increase in 1000 grain weight followed by PBW343 in the second season. Moreover, PBW34 and PBW343 gave the highest average of 50 spike weight in the second season.&nbsp

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