Hydatid cyst disease is one of the most common diseases in many places in the world. The infection occurs when human and livestock drinking or eating contaminated water and food with eggs of Echinococcus granulosus worm. Surgery is the best solution to eradicate cysts and rapid healing, but it may be accompanied by some risks such as rupture of the cyst and leakage its contents of protoscolices, which leads to the return of infection and spread in the body. Several methods have been used to reduce the risks of surgery, including withdrawal of hydatid fluid and its contents and injection scolicidal substances like ethanol and others. Researchers have recently tested the efficiency of nanoparticles such as selenium, silver, and gold nanoparticles against the protoscolices. The present study aims to test the efficiency of Titanium oxide (TiO2) nanoparticles versus protoscolices of hydatid cyst. Protoscolices were obtained from hydatid cysts in sheeplivers. The TiO2nanoparticles were prepared at concentrations of 250, 500, 1000, 2000, and 4000 µg/ml at different times (15, 30, 60 min.). The results showed the efficacy of all concentrations at all times in the killing of protoscolices compared with control groups.
