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Publication Date
Wed Mar 31 2021
Journal Name
Journal Of Tikrit University For Humanities
Volume
28
Issue Number
3, 4
DOI
10.25130/jtuh.28.3.4.2021.18
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4hiMCJgBVTCNdQwCEKlB
أثر الانتماء التاريخي للأندلسيين على تعزيز الثقافة الوطنية بعد سقوط غرناطة الحياة الاجتماعية أنموذجاً
Historical Belongingness
Andalusians
National Culture
Granada
Wijdan Fareeq Enad
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تم التوقيع على معاهدة غرناطة في 25 نوفمبر 1491 ، ومنحت فيها غرناطة جميع الحقوق الدينية والاجتماعية والاقتصادية ، ولكن على الأرض لم تكن سوى ورقة وخدعة ، والغرض منها استسلام المدينة في أقصر وقت. وبأقل التكاليف. وقد تعرض الأندلسيون للاضطهاد في جميع مناحي الحياة حتى جاء قرار طردهم جاء من البلاد.

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Publication Date
Mon Apr 06 2020
Journal Name
August
INFLUENCE OF SOME FACTOR ON SOMATIC EMBRYOS INDUCTION AND GERMINATION OF DATE PALM CV BARHI BY USING CELL SUSPENSION CULTURE TECHNIQUE
This study was done to examine different concentrations of mercuric chloride (Hgcl2) for Explants sterilization and different concentration of NAA) mg/L with 2iP for their effect on initiation primary callus and subsequent embryogenesis in Phoenix dactyliferae cultivar Barhi. Leaf primordial and shoot tips were excised from 2-3 years old offshoot. The result showed that contamination percentage decreased with an increase in concentration of Hgcl2 for two explants where in the control (0.0)% gave highest value 75% and 62.5% of contamination for leaf primordial and shoot tip respectively while the addition (0.1)% gave a significant decrease 37.5 and 0% for explants respectively. The Explants were inoculated onto Murashiege and Skoog
1962 (MS) medium supplemented with 2iP 3mg/L and containing 0
25
50 or 100 mg/L of NAA
the addition (50) mg/L of NAA gave highest value 68.8% in percentage of callus initiation with significant difference from addition 0% gave lowest value (18.8)%
as well Explants of shoot tip gave significant difference was 53.1% while leaf primordial gave 28.1%
Primary callus was transferred to fresh MS medium containing (0
5
10 and 20) mg/L of NAA with 3mg/L 2iP
Results were recorded after 12 weeks. A highest value of embryonic callus induction percentage or fresh weight and dry weight 87.5% or 1.56g and 0.47g for the character respectively for the treat 20 mg/L while control treatment 0.0 gave the lowest value 12.5% or 0.37g and 0.14g for same character respectively. Inoculated 1g fresh biomass into a 250 ml Erlenmeyer flask containing 50 ml MS liquid medium supplemented with NAA 20mg/L and agitated at 150 rpm by shaker to making suspension culture the measurements of packed cell volume ( pcv) or fresh and dry weight taken weekly for 12 weeks
maximum values were reached in the eighth week of PCV and fresh weight but the dry weight reached to highest value in seventh week
then the curve began to decline gradually until it reached stability in the last two weeks. The cell suspension was Diluted to different initial cell density of 100
500
10000
50000 and 100000 cells /ml with hemocytometer. The initial cell number of (10000) cells gave significant different of plating efficiency PE (11.6)% while (100) cells gave lowest value 1.8% of PE.
Ali A.
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