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Pseudomonas aeruginosa, a ubiquitous environmental
organism, is a difficult-to-treat opportunistic pathogen
due to its broad-spectrum antibiotic resistance and its ability to
form biofilms. In this study, we investigate the link between
resistance to a clinically important antibiotic, imipenem, and
biofilm formation. First, we observed that the laboratory strain
P. aeruginosa PAO1 carrying a mutation in the oprD gene,
which confers resistance to imipenem, showed a modest reduction
in biofilm formation.We also observed an inverse relationship
between imipenem resistance and biofilm formation for
imipenem-resistant strains selected in vitro, as well as for clinical
isolates.We identified two clinical isolates of P. aeruginosa from
the sputum
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