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In vitro investigation of phytochemical, antioxidant and antimicrobial activities of Harpagophytum procumbens seeds extracts

Medicinal plants contain bioactive substances that are highly bioavailable in extracts or pure molecules, making them promising for therapeutic applications and precursors for chemo-pharmaceutical semi-synthesis. Harpagophytum procumbens (Devil’s Claw) is widely recognized as one of the most potent therapeutic herbs. This study aimed to extract seeds from H. procumbens using two types of solvents and to assess both qualitative and quantitative aspects of the extracts. The two extracts were evaluated for antibacterial and anti-biofilm activities using agar well diffusion assays against four bacterial isolates and two yeast isolates. Qualitative analysis identified the presence of alkaloids, flavonoids, tannins, saponins, and terpenoids. The active components detected were: alkaloids (12.69%), flavonoids (3.25%), total phenolic compounds (24.58%), total terpenoids (8.55%), and total steroids (1.25% for methanolic and 4.55% for petroleum ether). Both methanolic and petroleum ether extracts exhibited antioxidant activities of approximately 85.33% and 74.19%, respectively, compared to ascorbic acid, which had an antioxidant effect of 67.99% at a concentration of 200 µg/ml. The extracts demonstrated a broad spectrum of activity against all tested bacteria (Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Streptococcus pyogenes) and fungi (Candida albicans, C. tropicalis, C. parapsilosis). At a concentration of 1000 µg/ml, the seed extracts showed the highest bactericidal activity, with inhibition zones ranging from 10 to 22 mm. Moreover, both extracts exhibited greater anti-biofilm activity at 1000 µg/ml compared to lower concentrations. Our study found that seed extracts of H. procumbens possess significant antibacterial and antioxidant activities, particularly at a concentration of 1000 µg/ml.

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Publication Date
Thu Aug 01 2013
Journal Name
Journal Of The College Of Basic Education
The antimicrobial activity of salvia pratensis extracts against some microbial agent

The effectiveness inhibitory to extract alcohol for the leaf and flower to plant sage Salvia pratensis each of Staphylococcus aureus, streptococcus epidermidis, Salmonella typhi, Pseudomonas aeroginosa, Escherichia coli, Aspergillus niger and Candida albicans whom had any inhibition to aqueous extracts of the parts itself species bacterial and fungal. The study also demonstrated that the extract of plant containing compounds chemical such as tannins, Alkaloids, Flavonoieds, and saponins, which owns effectiveness of medical. The MIC, MBC and inhibition zones for crud extract were determinated for microbial agents.

Publication Date
Thu Dec 01 2022
Journal Name
Russian Journal Of Bioorganic Chemistry
Synthesis, Identification and Study of Antioxidant and Anticancer Activities of New 2-Substituted-1,3,4-Thiadiazole from Creatinine

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Publication Date
Tue Jun 20 2023
Journal Name
Baghdad Science Journal
Synthesis and Evaluation of Platinum Nanoparticles Using F. Carica Fruit Extract and Their Antimicrobial Activities

Abstract

In this manuscript, a simple new method for the green synthesis of platinum nanoparticles (Pt NPs) utilizing F. carica Fig extract as reducing agent for antimicrobial activities was reported. Simultaneously, the microstructural and morphological features of the synthesized Pt NPs were thoroughly investigated. In particular, the attained Pt NPs exhibited spherical shape with diameter range of 5-30 nm and root mean square of 9.48 nm using Transmission Electron Microscopy (TEM) and Atomic Force Microscopy (AFM), respectively. Additionally, the final product (Pt NPs) was screened as antifungal and antibacterial agent against Candida and Aspergillus species as well as Gram-positive Staphyllococcus aureus and G

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Publication Date
Mon Sep 19 2022
Journal Name
International Journal Of Biomaterials
Green Biosynthesis of Silver Nanoparticles from Moringa oleifera Leaves and Its Antimicrobial and Cytotoxicity Activities

The plant occupied the largest area in the biosynthesis of silver nanoparticles, especially the medicinal plants, and it has shown great potential in biotechnology applications. In this study, green synthesis of silver nanoparticles from Moringa oleifera leaves extract and its antifungal and antitumor activities were investigated. The formation of silver nanoparticles was observed after 1 hour of preparation color changing. The ultraviolet and visible spectrum, Fourier transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, and transmission electron microscopy techniques were used to characterize synthesis particles. Ultraviolet and visible spectroscopy showed a silver surface plasmon resonance band at 434

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Publication Date
Sun Mar 05 2017
Journal Name
Baghdad Science Journal
Study the Effects of Olive Leaves Extracts in the activity of the enzyme GOT and their Biological Activities

The olive tree, has been used it is important plant for the time being some of their parts on a large scale in the treatment of gastrointestinal disorders and stimulate circulation . Moreover, it is used as antibacterial material and also to address some of the respiratory system, diabetes, food preservation osteoporosis. This study involved the collection of olive leaves from different areas in Baghdad / Iraq. These leaves have been harvested, wash it, then dried and crushed, where the study aimed to identify the active ingredients and chemical elements in the olive leaf as well as its effect on the action of GOT enzyme .The study showed that the aqueous extracts (cold and hot) of the olive leaves powder are acidic in nature pH values

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Publication Date
Mon Mar 02 2015
Journal Name
Journal Of Baghdad College Of Dentistry
Antibacterial Effects of Green Tea Extracts on Aggregatibacter Actinomycetemcomitans: In-Vitro Study

Publication Date
Thu Oct 01 2015
Journal Name
Journal Of Dental And Medical Sciences
Antibacterial effects of green tea extracts on Prophyromonas Gingivalis (In-Vitro study)

Publication Date
Fri Nov 22 2024
Journal Name
Journal Of Baghdad College Of Dentistry
Antibacterial effects of green tea extracts on Aggregatibacter actinomycetemcomitans (In-Vitro study)

Background: Green Tea is made from the leaf of the plant “Camellia sinensis”. Green tea is reported to contain thousands of bioactive ingredients including catechins which have shown great promise for having antimicrobial effects. Periodontal diseases represent one of the most prevalent diseases around the world and the main etiologic factor behind it, is plaque accumulation, in addition certain kinds of bacteria have been detected frequently in subjects suffering from periodontitis, Several studies suggested that the outcome of periodontal treatment is better if particular pathogens including Aggregatibacteractinomycetemcomitans can no longer be detected after therapy. Materials and Methods: plaque samples were collected from 2

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Publication Date
Fri Jul 28 2017
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Inhibition of Bacterial Growth by Lawsonia inermis (henna) Leaf Extracts In Vitro

Leaf samples of Lawsonia inermis were collected from Basrah city, South of Iraq to examine their antimicrobial activity . The effects of water and chloroform crude extracts of the leaves in different concentrations were obtained and bioassayed in vitro for its bioactivity to inhibit the growth of six types of bacteria . The extract of water was clearly superior for all bacteria especially the bacteria Staphlylococcus aureus (inhibition zone was 21mm in concentration 70mg/ml) from gram positive bacteria, and Klebsiella pneumoniae (inhibition zone was 20mm in the same concentration)  , and the growth of all bacteria was inhibited to varying degrees by increasing the concentration of the henna leaves and are commonly known to possess a

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Publication Date
Sun Sep 01 2013
Journal Name
Baghdad Science Journal
Spectroscopic Characterization and Antimicrobial Activities of Some Heavy Metals Complexes of 2-hydroxy phenyl piperonalidene

An antibacterial and antifungal piperonal-derived compound and its Rh(III), Pd(II), Pt(IV), and Cd(II) metal complexes were synthesized and characterized by spectroscopic methods, conductivity, metal analyses and magnetic moment measurements. The nature of the complexes formed in ethanolic solution was studied following the molar ratio method. From the spectral studies, octahedral geometry was suggested for rhodium (III) and platinum (IV) complexes, while a square planer structure was suggested for palladium (II) complex and a tetrahedral geometry for cadmium (II) complex. Structural geometries of these compounds were also suggested in gas phase by using hyperchem-8 program for the molecular mechanics and semi-empirical calculations.

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