The aim of the study was molecular detection of C. neoformans that isolated from 150 (88 female and 62 male) clinical samples (sputum samples) from pulmonary patients in Baghdad. The diagnoses of Cryptococcus neoformans in samples was done by using direct microscopic examination, culture media and PCR Technology. Microscopic examination and cultured revealed that 65 out of 150 (43.33 %) samples were positive and the others samples were Negative. Results of the genetic diagnosis looking for the fungi causing cryptococcosis using primers specific for ITS gene which were specially designed for this study revealed that 6 (4 %) of sputum samples were positive. In this study used the PCR technology due to the present

Background: Septicemia is a common condition in children with a resultant high morbidity and mortality. It’s defined as the presence of bacteria in the blood and is often associated with severe infections, the alternative names (Blood poisoning, Bacteremia with sepsis, systemic inflammatory response syndrome).
Objective: To study the etiological agents and antibiotics susceptibility of septicemia in children admitted to Central Teaching Hospitals of Pediatric.
Patients and Methods: A total of 80 patients having signs and symptoms of Septicemia who were admitted to Central Teaching Hospital of Pediatric in Baghdad city. Samples were obtained during the period from the 1st January till the 30 th of Feb

Fisetin is a plant flavonoid found in strawberries and other fruits and vegetables such as apples, persimmons, and onions. It has many pharmacological effects like anti-inflammatory, antioxidant, cardioprotective, neuroprotective, and anti-carcinogenicity which are attributed to its ability to reduce oxidative stress which considers the main reason for different disease conditions. Genotoxicity refers to the genetic material destruction within the cell which can be caused by different chemicals as well as radiation. The present study evaluates the effect of orally-administered fisetin daily for seven constitutive days on genotoxicity induced by cyclophosphamide in rats’ bone marrow and spleen cells. Results showed that fisetin exh

E. coli was isolated, and it was Gram-negative rod bacteria that was colony circular, regular edged, thick somewhat glitter and  viscous(less). It was lactose fermenter bacteria and belongs the family of Enterobacteriaceae. E. coli showed sensitivity to all used antibiotics except Erythromycin (E), Cloxacellin (CX), Rifampin (RA), Cephalothin (KF), Ampicillin (AM), and Penicillin (P). The experimental results of antibiotic sensitivity of E. coli in media containing different concentrations of omeprazole, a proton pump inhibitor, showed an enhancement of resistance by decreasing the sensitivity of E. coli inversely with drug concentration against the antibiotics that E. coli was sensitive to. It seems that omeprazole changed cell mem

Lipopolysaccharide (LPS) of Campylobacter coli was extracted by using
digestive enzyme and hot phenol method. The effect of LPS on lymphocyte
transform was studies by lymphocyte transformation index for twenty blood samples
were collected from apparently healthy control.
The results were showed significant differences (P< 0.05) between samples which
treatrd with phytohemagglutnin PHA (66.1 ± 0.6) and the samples which treated
with LPS of C. coli (74.2 ± 0.8) when compared with control, this lead to suggest
that the LPS extracted from C. coli may play a role as a mitogen to transformed
lymphocytes.

       The objective of this study is to test In Vitro the twenty chemical compounds that contains Schiff base or oxazepine, indoline, imidazolo units in concentrations( 50, 100, 150) mg / dl as antifungal activity, against three pathogenic Candida species  that occur in humans. We tested one isolates of

   ) Candida albicans ,Candida glabrata and Candida krusei). All these species affect human health . The study was carried out in the Laboratory of Public Health , directly of health for the period from May 2016 to April 2017  , Candida spp isolates used in this study were collected from patients admitted at  some private c

Background: Removal of bacteria from the pulp system by instrumentation of an infected root canal, will be significantly reduced the number of bacteria, but it is well documented that instrumentation alone can-not clean and kill all bacteria found on the root canal walls. Antibacterial irrigants are needed to kill the remaining microorganisms. The aims of this study was to assess antibacterial effect of titanium tetrafluoride (TiF4) solution and brewing green tea against root canal bacteria and to compare with sodium hypochlorite and normal saline through microbiological and molecular studies. Materials and methods: Microbiological study was carried out to determine the concentration of titanium tetrafluoride and brewing green tea at which

Grape is a phenolics rich plants, Black Grape seed extract was reported to have many pharmalogically benefits including antioxidant, antimicrobial, anticancer and anti aging and anti Alzheimer's properties. Black grape seed phenols were extracted by percolation method. Results showed that the minimum inhibitory concentration of phenolic extract had no effect against K.pneuminae, However, variable inhibitory effects were developed against pathogenic P.aeruginosa , E.coli ,Bacillus ,S.aureus , E.faecalis and C.albicans. We found that black grape seed extract (Vitis vinifera) was more effective against gram positive bacteria than gram negative bacteria and its effect also against pathogenic C.albicans.