metoclopramide hydrochloride
diazotization
pharmaceutical preparations
spectrophotometry
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A UV-Vis spectrophotometry method was developed for the determination of metoclopramide hydrochloride in pure and several pharmaceutical preparations, such as Permosan tablets, Meclodin syrups, and
Plasil ampoules. The method is based on the diazotization reaction of metoclopramide hydrochloride with
sodium nitrate and hydrochloric acid to yield the diazonium salt, which is then reacted with 3,5-dimethyl
phenol in the presence of sodium hydroxide to form a yellow azo dye. Calibration curves were linear in the
range from 0.3 to 6.5 µg/mL, with a correlation coefficient of 0.9993. The limits of detection and quantification were determined and found to be 0.18 and 0.61 µg/mL, respectively. Accuracy and precision were also
determined b
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