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Evaluation of Quorum-Sensing, Antibiotics Resistance, and Biofilm Formation in Pathogenic Bacteria from the Hospital Environments
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Abstract Background: Multidrug-resistant bacteria (MDR) often contaminate hospital environment and cause serious illnesses. Quorum Sensing (QS) regulates a variety of downstream cellular processes, including antibiotics resistance mechanisms and biofilm formation, and causes harm to the host. This study investigates antibacterial susceptibility and biofilm formation of pathogenic bacteria in hospital environment. Methods: Hundred bacterial isolates were collected from various environments in the Medical City hospital. The antimicrobial susceptibility technique was evaluated through disk diffusion method. Next, biofilms formation was detected by the microliter plate assay. Finally, PCR was used to analyze the frequency of QS system genes. Results: Current findings showed that the predominant isolates were Acinetobacter baumannii (34%), Escherichia coli (30%), Pseudomonas aeruginosa (19%), and Klebsiella pneumonia (17%). In general, significant resistance was found related to trimethoprim (88%), Augmentin (88%), and cefotaxime (72%). Among all isolates, 62% of sensitivity was related to ciprofloxacin. Biofilm had been formed by 39% of isolates. PCR results showed that the frequency of lasI and rhlI gene was 70% and 61%, respectively. Conclusion: Current findings revealed that the hospital environment is a potential reservoir of MDR gram-negative pathogenic bacteria. Thus, we suggest that the health policymakers in Iraq must critically apply the guidelines and recommendations for monitoring the environments in the health sector. Keywords: Antibiotics Footprint, Acinetobacter baumannii, Antibiotics Resistance, Quorum-Sensing, PCR.

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Publication Date
Sun Jul 30 2023
Journal Name
Iraqi Journal Of Science
Screening of periodontal disease related Bacteria in Iraqi patients and their relationship with salivary TLR2 and IL-6 level
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     The oral cavity has the second major and most diverse microflora after the gut. Since it is the starting point of digestion; the oral microbiota is essential for maintaining oral and systemic health.  Thus, this study aimed to find the bacterial isolates which act as a causative agent of periodontal diseases in Iraqi patients and their relation with some immune oral parameters. Saliva specimens and oral swabs of 91 patients (51) and control individuals (40) were collected in this study. The specimens of patients were collected from Al-Amirya and Almaamon Specialized Dental Centers in Baghdad for a period from November/2021 to February/2022. The microbiological results revealed, that the most prevalent bacterial isolates were re

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Publication Date
Tue Dec 30 2008
Journal Name
Al-kindy College Medical Journal
Three Years Experience in the Management of Uterine Rupture at Al-Battool Teaching Hospital Mosul-Iraq
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Background: Ruptured uterus is a catastrophic event to both
mother and her fetus. Apart from maternal and fetal mortality
rates, the incidence of rupture of uterus is often taken as an
index of the standard of obstetric care.
Objective: To determine the frequency, causes, management
outcome of ruptured uterus at Al Batool maternity hospital.
Method: The study was conducted in the department of
obstetrics and gynecology at Al Batool maternity hospital
Mosul-Iraq over a period of three years from October 1st 2002
to August 30th 2005. All the cases of uterine rupture presented
during the study period were recorded and managed in the
department. Data was recorded on designed forms.
Results: Thirty nine cas

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Publication Date
Tue Dec 30 2014
Journal Name
Scholars Academic Journal Of Biosciences (sajb)
Prevalence and Evaluation of Antimicrobial Activity of Dodonaea viscosa Extract and Antibacterial Agents against salmonella Spp. Isolated from Poultry
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A total of 200 samples (180 fecal materials and 20 organ samples) were collected from (5 different poultry farms, 10 local poultry shops, 5 houses poultry, 5 Eggs stores shops and 5hand slaughters centers) in Ibb city, Yemen, 2014. According to morphological, cultural, as well as biochemical characterization and serological tests, 59(29.5%) isolates were identified as Salmonella spp. and all Salmonella isolates were categorized by serotype, which comprised of, 37(62.71%) Salmonella Typhimurium serovar, 21(35.59%). Salmonella Enteritidis serovar and 1(1.69%) Salmonella Heidlberg serovar. Antibiotic sensitivity test was done for bacterial isolates and the results showed there were clear differences in antibiotic resistant. Antimicrobial

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Publication Date
Sun May 01 2016
Journal Name
Arabian Journal Of Geosciences
Iron mineralization in the Garagu Formation of Gara Mountain, Duhok Governorate, Kurdistan, NE Iraq: geochemistry, mineralogy and origin
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Publication Date
Mon Dec 06 2021
Journal Name
Indian Journal Of Forensic Medicine & Toxicology
Assessment the Complications of Caesarean Section among Women’s attending AL -Diwaniyah Maternity and Pediatric Hospital/Iraq
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Aims: This study aimed to assess complications of caesarean section among pregnant women attending AL Diwaniyah Maternity and Pediatric Hospital; and find out relationship between complications of caesarean section and socio-demographic data of women. Methods: A descriptive cross-sectional study design is conducted for the period of December 26th 2020 to June 1st 2021. The validity of the questionnaire is determine through a panel of experts and reliability was achieved through a pilot study. By a purposive sample is selected among pregnant women who undergo caesarean section, data was collected through the use of questionnaire and interview techniques; and analyzed through the descriptive and inferential statistic. Results: Findings indica

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Publication Date
Sat Oct 01 2022
Journal Name
Journal Of Medicine And Life
The effect of COVID-19 on emergencies and pain among orthodontic patients attending a teaching hospital
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This study aimed to evaluate the effect of the COVID-19 outbreak on emergencies and pain among orthodontic patients attending a teaching hospital. The study was conducted among orthodontic patients receiving active orthodontic treatment or in a retention period at the College of Dentistry, University of Baghdad, Iraq. Their participation was voluntary, and they filled out an Arabic-translated questionnaire. The survey included general information, orthodontic problems, and a numerical rating scale for pain assessment. We used descriptive and inferential statistics (frequencies and intersecting frequencies), chi-square test and linear regression. Out of 75 orthodontic patients, only 54 (15 males and 39 females) were included in the s

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Publication Date
Mon Jan 01 2024
Journal Name
Lecture Notes In Networks And Systems
Implementing a Smart Low-Cost System for Diagnosing Bacteria in Women
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Publication Date
Wed Jan 01 2020
Journal Name
Pakistan Journal Of Agricultural Sciences,
Evaluation of potent silver nanoparticles production from agaricus bisporus against helicobacter pylori
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Publication Date
Wed Jan 01 2014
Journal Name
Journal Of Engineering Geology And Hydrogeology
Assessing Pullout Resistance of Earth Reinforced Embankment Model
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- The sandy soil with high gypsum content (usually referred to as gypseous soil) covers vast area in south, east, middle and west regions of Iraq, such soil possess a type of cohesive forces when attached with optimum amount of water, then compacted and allowed to cure, but losses its strength when flooded with water again. Much work on earth reinforcement was published which concentrate on the gain in bearing capacity in the reinforced layer using different types of cohesive or cohesion less soil and various types of reinforcement such as plastic, metal, grids, and synthetic textile. Little attention was paid to there enforce gypseous soil. The objective of this work is to study the interaction between such soil and reinforcement strips

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Publication Date
Sun Mar 06 2011
Journal Name
Baghdad Science Journal
Laser Effect on Fatique Resistance of Carbon Steel
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Laser beam has been widely used to improve the mechanical properties of the metals. It used for cutting, drilling, hardening, welding……etc. The use of Laser beam has many features in accuracy and speeding in work, also in the treatment of metals locally, and in the places that is hard to reach by traditional ways. In this research a surface treatment was done to medium carbon steel (0.4%C) which is common kind of steel that is used in industry. Pulsing Neodymium -YAG Laser has been used and 1.06 micrometer wave length and 5 msec and the distance is about 30 centimeter between the exit area of the Laser beam from the system and the piece that treated . We are going to check the fatigue resistance for samples that is

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