The purpose of this study to synthesize and characterize silver nanoparticles using phenolic compounds obtained from Camellia sinensis, to test the antibacterial properties of biosynthesized nanoparticles on the formation of biofilms in multidrug-resistant Pseudomonas aeruginosa. Ten isolates of P. aeruginosa were obtained from the Genetic Engineering and Biotechnology Institute laboratories of the University of Baghdad. By using the VITEK-2 system and culturing the isolates on cetrimide agar, the diagnosis was confirmed. Camellia sinensis silver nanoparticles (CAgNPs) were created using an extract of the plant's aqueous and methanolic leaves. Based on the results of the nanoparticle synthesis, spherical nanoparticles that may be single or mixed were included in both the aqueous and methanolic extracts of silver nanoparticles. By comparing their retention times to those of the reference compounds, the HPLC findings revealed that two phenolic compounds (gallic acid and caffeine) had been discovered. Utilising the disc diffusion technique, the antibacterial activity of (CAgNPs) was assessed. The results indicated that the methanolic (CAgNPs) extract was more effective than the aqueous (CAgNPs) extract at 375 and 750 ppm, giving the highest inhibition zone 17.67 and 21.33 mm, respectively, when compared to the aqueous (CAgNPs) extract, which produced inhibitory zones 13.00 and 16.33 mm, respectively. The MIC findings indicated that the methanolic CAgNPs extract was more effective than the aqueous CAgNPs extract; the MIC of the methanolic CAgNPs extract was 23.43 μg/ml in all P. aeruginosa isolates, except the isolates No. 9 and 10, which was 11.718 μg/ml. While in the aqueous extract, the MIC in all P. aeruginosa isolates was 187.5 μg/ml, with the exception of isolates No. 9 and 10, which was 93.75 μg/ml. Additionally, the methanolic CAgNPs extract entirely inhibited P. aeruginosa from building a biofilm when used at 23.43 μg/ml. However, at 46.87 μg/ml of the aqueous CAgNPs extract, totally reduced the biofilm forming activity on P. aeruginosa isolates
The tax is a financial amount collected from the taxpayer for the account of the public treasury.. and is the cornerstone of the close bond between members of the community and the country in which they live , as income tax is imposed on the profits and revenues achieved by people and often the constitution of most contemporary countries stipulate that the legislator is obligated to determine the sources of revenues and profits subjected to tax. Because the tax has a serious impact on people’s money because it is exposed to that money through direct deduction . Therefore, we note that the applicable income Tax Law stipulated that in order income to be subject to tax , it must be derived from one or more of the income sources st
... Show MoreBackground: Recurrent aphthous ulceration (RAU) is an inflammatory condition of unknown etiology characterized by painful recurrent (single or multiple) ulcerations of the oral mucosa. It is one of the most common and poorly understood mucosal disorders. It occurs more frequently in times of stress. Local and systemic conditions, genetic, immunologic, microbial factors, and oxidative stress may play a role in the pathogenesis of RAU. The objective of this study was to evaluate the free radical metabolism and antioxidant activity of RAU patients treated by lavender or flax oil paint.
Materials and Methods: Sixty-six RAU patients were enroll
... Show MoreBackground: Pain is one of the most postoperative complications of surgical wound especially within first 24 hrs. leading to delay hospital discharge, stress gastritis and increasing blood pressure. As wound infiltration with long acting local anesthetic (bupivacaine) has been proved to be effective after orthopedic surgeries especially total hip and knee replacements.Objective: our study was designed to determine theeffectiveness of local infiltration of 0.5% of bupivacainebefore closure of surgical wounds in controllingpostoperative pain and improve patient’s outcome after totalhip and knee replacement surgeries in first 24hrspostoperative period.Methods: Twenty patients from class I (healthy patients) and class II (patients mild sys
... Show MoreAbstract
To evaluate the effectiveness of Phenolic Compound of Salvia Frigida on induced atopic dermatitis (AD) of mice. Forty mice were included in the study, divided in to four groups (10 mice/group): apparently healthy, induced AD without treatment, induced AD treated with tacrolimus 0.1% ointment, and induced AD treated with Phenolic Compound of Salvia Frigida cream 5%. Examination of histopathology was done and skin homogenates levels also measured. Levels of WBC, Eosinophil, skin tissue homogenate of IL-13 and IL-4, serum IgE, and histopathological scores were significantly increased among induced non treated AD group in comparison with control group. Comparisons of non-treated induced AD
... Show More Fusobacterium are compulsory anaerobic gram-negative bacteria, long thin with pointed ends, it causes several illnesses to humans like pocket lesion gingivitis and periodontal disease; therefore our study is constructed on molecular identification and detection of the fadA gene which is responsible for bacterial biofilm formation. In this study, 10.2% Fusobacterium spp. were isolated from pocket lesion gingivitis. The isolates underwent identification depending on several tests under anaerobic conditions and biochemical reactions. All isolates were sensitive to Imipenem (IPM10) 42.7mm/disk, Ciprofloxacin (CIP10) 27.2mm/disk and Erythromycin (E15) 25mm/disk, respectively. 100% of
Experimental tests were carried to control lost circulation in the Khabaz oil field using different types of LCMs including Nano-materials. A closed-loop circulation system was built to simulate the process of lost circulation into formations. Two dolomite plugs were used from different depths of the formation of Azkand in Khabaz oil field. The experimentations were carried out to study the effect of different types of LCMs, cross-linked copolymer (FLOSORB CE 300 S), SiO2 NP, and Fe2O3 NP, on mud volume losses as a function of time.
The rheological measurements of the nanoparticles-reference mud system showed that both of the SiO2 NP and Fe2O3 NP w
... Show MoreBackground: Physical exercise is essential for performance of the immune system. The integrity of immune response is dependent on the intensity and duration of the exercise performed. During exercise the metabolic and hormonal changes that occurred can induce lymphocyte apoptosis. Dehydroepiandrosteron sulphate (DHEAS) is one of the hormones that is affected by exercise, it is an androgen released from the adrenal cortex in response to adrenocortiocotropin releasing hormone (ACTH). DHEAS is regarded as an anti-aging hormone; it is primarily immune enhancing, providing some degree of counterbalance to the potentially detrimental effects of catecholamine and cortisol which are immunosuppressive if chronic
... Show More