The high mobility group A1 gene (HMGA1) rs139876191 variant has been related to metabolic syndrome and type 2 diabetes, but data are lacking in Middle Eastern populations. The study aimed to assess whether the HMGA1 rs139876191 variant is associated with metabolic syndrome risk and whether this variant predicts the risk of insulin resistance. This case-control study was carried out at single center in Kirkuk city/ Iraq from February to August 2022. Polymorphisms in HMGA1 and genotyping were identified by Sanger sequencing of genomic DNA obtained from 91 Iraqi participants (61 patients with metabolic syndrome and 30 control). Lipid profile, serum (glucose and insulin), glycated hemoglobin, blood pressure, body mass index, and waist circumference were also measured. The high prevalence of the del/del genotype of rs139876191 was found. Minor allele frequency of rs139876191 was 0.16 in both metabolic syndrome and the control group. A non-significant difference in genotyping was identified between total metabolic syndrome and the control group. The del/ins variant was associated with significantly higher waist circumference, triglycerides (TG), low-density lipoprotein (LDL), very low-density lipoprotein (VLDL), and glycated hemoglobin (HbA1c) (P=0.03, 0.041, 0.007, 0.034, and 0.001, respectively), and significantly lower high-density lipoprotein (HDL) (p=0.000). Linear regression analysis showed no significant effect of the variant (del/ins) on developing insulin resistance. Thus, rs139876191 polymorphism with del/ins genotype in the HMGA1 gene was not associated with metabolic syndrome risk but it was associated with indices of metabolic syndrome including waist circumference, TG, HDL, LDL, VLDL, and HbA1c. Besides, this variant did not predict the risk of insulin resistance
Background: Among different air pollutants, cigarette smoke contains toxic chemicals, mutagenic and carcinogenic compounds, which can adversely affect male fertility. In this study, semen parameters and reproductive hormonal concentrations of subfertile smokers were compared with subfertile non-smokers.
Objectives: evaluation of the effect of cigarette smoking on male fertility by evaluating several semen parameters as well as some reproductive hormones in a group of smoker and non smokersubfertile Iraqi subjects.
Patients and Methods: At the male infertility clinic of Al-yarmuk teaching hospital, Almustanseria medical college, Baghdad, Iraq from the 1st of October 2010 to the end of June 2011, 88 men (49 non-smokers, and 39 smoker
Nine Iraqi varieties of barley (Hordeum vulgare L.) has been differentiated and diagnosed using simple sequence repeat markers to detect their genetic polymorphism. Six SSR primers were used for genetic screening of barley samples (IPA 265, IPA 99, Tuwaitha, Hitra, Rayhan, Shuaa, Bawadi, Samir and Al_khair). These primers generated total PCR product (11) bands divided to 8 polymorphic bands 3 monomorphic bands. the percentage of polymorphism 80% ranged between (50-100%). a mean value of polymorphic band per primer was 1.6 . these primers produced amplification fragment at Molecular weight between 75-900 bp. One unique band was generated at size 200bp, this band can be used as a DNA profiling of all studied genotypes. These results appear
... Show MorePolycystic ovary syndrome (PCOS) is the main cause of female infertility. The role of insulin resistance in the development of polycystic ovary is actively discussed here. The study included patients with PCOS without insulin resistance (n = 48) and with insulin resistance (n = 39). The comparison groups were patients with no history of PCOS: a control group without insulin resistance (n = 46) and a group of patients with insulin resistance (n = 45). The following parameters were determined in patients: FSH, LH, TSH, T3f, T4f, PRL, E2, 17-OHd, Pr, AMH, Test total, Testf, DHEAS, DHEASs, SHBG, ACTH, cortisol, IRI, IGF-1, C-peptide, and glucose level. The HOMA-IR index and the LH / FSH ratio and t
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AIM: The aim of this study was to measure the prevalence of myeloproliferative disorders in a sample of Iraqi patients and to measure the changes in patients’ blood parameters. BACKGROUND: Myeloproliferative disorders are a group of neoplasms affecting the bone marrow progenitor cells characterized by excess cells with a risk of transforming to acute leukemia. There is a gap in knowledge about the prevalence of Iraqi population. Thus, we investigated the prevalence and distribution of different types of myeloproliferative disorders in a sample of Iraqi patients. MATERIALS AND METHODS: Cross-sectional study is done at the National Center of Hematology from November 2019 till March 2020 on 75 patients who were diagnosed
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In spite of the high rate of morbidity and mortality heart failure (HF) is common, and none of the medications are now entirely available for HF treatment. In addition to many environmental influences and clinical diseases, genetic factors may also contribute to the progression and development of HF. In the current study, samples of blood were collected from 150 heart failure patients and 130 healthy controls. We evaluated the association of four single nucleotide polymorphisms (snps) of Toll-like receptors (TLR6 and TLR5) with (HF) susceptibility in the Iraqi population. In this work, (SNP) called Toll-like receptor 5 (rs5744168, rs2072493) and Toll-like receptor 6 (rs1039559, rs5743810) were employed. (PCR-RFLP) for snps
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Background: Metabolic syndrome is a cluster of risk factors for atherosclerotic cardiovascular disease caused by abdominal obesity, such as hyperglycemia, hypertension, dyslipidemia, and insulin resistance.Adiponectin is a protein hormone that modulates a number of metabolic processes, including glucose regulation and fatty acidcatabolism. Adiponectin is exclusively secreted from adipose tissue into the bloodstream. Leptin, a hormone synthesized by fat tissue had been noted to regulate energy balance and metabolism.In this study investigated the relationships of adiponectin/leptin ratio with metabolic syndrome in apparently healthy Iraqi male adults.
Objective: This study was designed to investigate the r
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Bitter substances are identified by protein receptors located on surface of taste cell membranes. Mutational polymorphism of the bitter taste receptor (TAS2R38) is a significant determinant in phenylthiocarbamide (PTC) threshold perception. This research's objectives were to find TAS2R38 polymorphisms in Iraqi people and investigate any correlations between genotype and the PTC taste sensitivity. Bitterness sensitivity was determined by assessing the capacity to differentiate and the responsiveness to a representative strip of PTC. Cheek cells samples were collected for DNA extraction, PCR amplification and genotyping. PCR was performed to amplify the short region of the TAS2R38 gene containing the initial polymorphisms of inter
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Abstract: Lymphoproliferative Disorders (LPDs) are a group of neoplasms affecting various cells within lymphoid system. Each type has different treatment a..70619
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