Inhalation of Staphylococcal Enterotoxin B (SEB) is known to induce acute lung injury (ALI) and studies from our laboratory have shown that THC, a psychoactive ingredient found in Cannabis sativa, can attenuate the ALI. In the current study, we investigated the role played by lung microbiota in ALI with or without THC treatment. A dual-dose of SEB was given to C3H/HeJ mice, which were then treated either with vehicle or THC. SEB-administration caused ALI and 100% mortality while all THC-treated mice survived and suppressed the inflammation in the lungs. Furthermore, lung microbiota was collected and 16S rRNA sequencing was performed. The data were analyzed to determine the alpha and beta diversity. The major phylum was Proteobacteria, class Alphaproteobacteria and orders were Caulobacterles and Rhodobacteralesin in the lungs of vehicle-treated SEB group. Moreover, THC treatment led to elevated Firmicutes phylum due to significant increase in the beneficial genus, Lactobacillus. Lipopolysaccharide (LPS), gram negative bacterial endotoxin was found at significantly higher concentration in the BALF of vehicle-treated group in comparison with THC-treated mice. Beneficial metabolome levels were significantly higher in the colonic flush of THC-treated mice when compared to vehicle-treated SEB group, specifically butyric, propionic and acetic acids. Together, our data suggest that THC attenuates SEB-induced mortality and ALI by altering the microbiota in the lungs (Supported by NIH grants P01AT003961, R01AT006888, R01ES019313, R01MH094755, P20GM103641, R01AI129788 to PN and MN and MoHESR fellowship for AKM).
Background: Non-host-adapted Salmonella serovar Typhimurium is a facultative intracellular bacterium, which invades and multiplies within mononuclear phagocytes in liver, spleen, lymph nodes and Peyer’s plaques. Salmonella infection is a crucial medical and veterinary problem globally. S. Typhimurium causes various clinical symptoms, from asymptomatic infection to typhoid-like syndromes in infants or highly susceptible animals, for instance mice. Objective: The present study was carried out to investigate the efficacy of anthrax protective antigen (PA)as a potent adjuvant mixed with killed Salmonella Typhimurium (S.T.) to enhance the immunization capacity of the last. Materials and Methods: Two groups of mice were immunized with either th
... Show MoreAcute respiratory distress syndrome (ARDS) is defined as a type of respiratory failure that is caused by a variety of insults such as pneumonia, sepsis, trauma and certain viral infections. In this study, we investigated the effect of an endocannabinoid, anandamide (AEA), on ARDS induced in the mouse by
Background: Acute coronary syndrome (ACS) is a common disease, and a major determinant of morbidity and mortality in all races. The pleiotropic effects of the receptor activator of nuclear factor-kappa B ligand (RANKL) such as modulation of cell survival, mineralization and inflammation, make it an interesting candidate mediator in the progression and destabilization of atherosclerotic lesions.Objectives: This study was performed to investigate the role of RANKL in the pathogenesis of ACS.Methods: The levels of RANKL were measured by ELISA method in sera of 60 ACS patients, 31 patients with unstable angina (UA) and 29 patients with myocardial infarction (MI) in comparison with 20 apparently healthy controls.Results: Current data indicate
... Show MoreThe definition of semi-preopen sets were first introduced by "Andrijevic" as were is defined by :Let (X , ï´ ) be a topological space, and let A ⊆, then A is called semi-preopen set if ⊆∘ . In this paper, we study the properties of semi-preopen sets but by another definition which is equivalent to the first definition and we also study the relationships among it and (open, α-open, preopen and semi-p-open )sets.
A class of hyperrings known as divisible hyperrings will be studied in this paper. It will be presented as each element in this hyperring is a divisible element. Also shows the relationship between the Jacobsen Radical, and the set of invertible elements and gets some results, and linked these results with the divisible hyperring. After going through the concept of divisible hypermodule that presented 2017, later in 2022, the concept of the divisible hyperring will be related to the concept of division hyperring, where each division hyperring is divisible and the converse is achieved under conditions that will be explained in the theorem 3.14. At the end of this paper, it will be clear that the goal of this paper is to study the concept
... Show MoreCzerwi’nski et al. introduced Lucky labeling in 2009 and Akbari et al and A.Nellai Murugan et al studied it further. Czerwi’nski defined Lucky Number of graph as follows: A labeling of vertices of a graph G is called a Lucky labeling if for every pair of adjacent vertices u and v in G where . A graph G may admit any number of lucky labelings. The least integer k for which a graph G has a lucky labeling from the set 1, 2, k is the lucky number of G denoted by η(G). This paper aims to determine the lucky number of Complete graph Kn, Complete bipartite graph Km,n and Complete tripartite graph Kl,m,n. It has also been studied how the lucky number changes whi
... Show MoreThroughout this paper R represents commutative ring with identity and M is a unitary left R-module. The purpose of this paper is to investigate some new results (up to our knowledge) on the concept of weak essential submodules which introduced by Muna A. Ahmed, where a submodule N of an R-module M is called weak essential, if N ? P ? (0) for each nonzero semiprime submodule P of M. In this paper we rewrite this definition in another formula. Some new definitions are introduced and various properties of weak essential submodules are considered.
In this paper we define and study new concepts of fibrewise topological spaces over B namely, fibrewise closure topological spaces, fibrewise wake topological spaces, fibrewise strong topological spaces over B. Also, we introduce the concepts of fibrewise w-closed (resp., w-coclosed, w-biclosed) and w-open (resp., w-coopen, w-biopen) topological spaces over B; Furthermore we state and prove several Propositions concerning with these concepts.
Throughout this paper R represents commutative ring with identity and M is a unitary left R-module. The purpose of this paper is to investigate some new results (up to our knowledge) on the concept of weak essential submodules which introduced by Muna A. Ahmed, where a submodule N of an R-module M is called weak essential, if N ? P ? (0) for each nonzero semiprime submodule P of M. In this paper we rewrite this definition in another formula. Some new definitions are introduced and various properties of weak essential submodules are considered.
The definition of semi-preopen sets were first introduced by "Andrijevic" as were is defined by :Let (X , ï´ ) be a topological space, and let A ⊆, then Ais called semi-preopen set if ⊆∘ . In this paper, we study the properties of semi-preopen sets but by another definition which is equivalent to the first definition and we also study the relationships among it and (open, α-open, preopen and semi-p-open )sets.