Carbon tetrachloride (CCl4) has been demonstrated to induce testicular damage via oxidative stress. Bromelain (Br), a proteolytic enzyme known for its biological activities and pharmacological properties, exhibits limited absorption owing to its low solubility and bioavailability. The present study aimed to investigate the histological and immunohistochemical effects of bromelain conjugated with gold nanoparticles (AuNPs) on the testis histology of albino mice treated with CCl4. Thirty-five male albino mice (Mus musculus), with an average age of 9 weeks, were randomly divided into five groups, each containing seven mice. The experiment was prolonged for four weeks. The first group (G1) was the control group, the second group (G2) received weekly sub-peritoneal injections of CCl4 until the end of the experiment, and the third group (G3) received an oral dose of Au-NPs solution. The fourth group (G4) received injections of CCl4; one hour later, the mice were given an oral dose of 300 mg/kg bromelain. The fifth group (G5) received an injection of CCl4; subsequently, the mice received the same oral dosage of 300 mg/kg Au-NPs-bromelain after 60 minutes. The mice's testes were sampled to evaluate histopathological alterations and immunohistochemical markers, particularly Ki-67 and caspase-9. The present results indicated that mice treated with CCl4 displayed a range of histological alterations, including testicular damage, degeneration in seminiferous tubules, vacuolation, and loss of germ cells. Furthermore, the immunohistochemistry study demonstrated that Ki-67 intensity decreased while caspase-9 intensity increased in groups G2 and G4 compared to the control group. Bromelain loaded with gold nanoparticles at a dosage of 300 mg/kg exhibited a notable reduction in the harmful effects of CCl4 on the testicular tissue of mice by restoring the histological structure, enhancing the Ki-67 proliferation protein, and suppressing the apoptotic cascade protein indicated by caspase 9. Keywords: Bromelain, Carbon tetrachloride, Caspase, Gold nanoparticle
Background: Green Tea is made from the leaf of the plant “Camellia sinensisâ€. Green tea is reported to contain thousands of bioactive ingredients including catechins which have shown great promise for having antimicrobial effects. Periodontal diseases represent one of the most prevalent diseases around the world and the main etiologic factor behind it, is plaque accumulation, in addition certain kinds of bacteria have been detected frequently in subjects suffering from periodontitis, Several studies suggested that the outcome of periodontal treatment is better if particular pathogens including Aggregatibacteractinomycetemcomitans can no longer be detected after therapy. Materials and Methods: plaque samples were collected from 2
... Show More
Anadrol (oxymetholone) is an active androgenic anabolic steroid that has been clinically studied in numerous diseases since the 1960s. It is used in the treatment of anemia and the replacement of male sex steroids. Unfortunately, in attempts to improve physical performance, Anadrol could be misused by athletes, that can lead to poisoning contributes to hepatotoxicity.
The aim of this study was to investigate the impact of anadrol on the liver function in rat model, via assessment of liver enzymes and histopathological study.
A forty male rats, weights about (200-300 gm), aged 8-12 weeks, after acclimatization, the rats were randomly divided into four groups (10 rats in each group) as follow: control group (in w
... Show MoreInfection with cryptosporidiosis endangers the lives of many people with immunodeficiency, especially HIV patients. Nitazoxanide is one of the main therapeutic drugs used to treat cryptosporidiosis. However, it is poorly soluble in water, which restricts its usefulness and efficacy in immunocompromised patients. Surfactants have an amphiphilic character which indicates their ability to improve the water solubility of the hydrophobic drugs. Our research concerns the synthesis of new cationic Gemini surfactants that have the ability to improve the solubility of the drug Nanazoxide. So, we synthesized cationic Gemini surfactants. N1,N1,N3,N3-tetramethyl-N1,N3-bis(2-octadecanamidoethyl)propane-1,3-diaminium bromide (CGSPS18) and 2,2‘-(etha
... Show MoreAnatomical changes in internal tissue of stem and leaf when seed and plant treated with acids to enhance growth and development in maize was studied during the spring seasons of 2019 and 2020. Randomized complete block design was used with three replications. Main plots received foliar nutrition treatments, including ascorbic acid (AA), citric acid (CA), and humic acid (HA) at concentrations of 100 mg L−1, alongside HA at 1 ml L−1, with distilled water as the control. Sub-plots underwent corresponding treatments for seed soaking. Results indicated variations in vascular bundle size among treatments, with foliar CA treatment showing superior results in both years, as well as seed soaking in CA and HA. Interaction effects were observed, n
... Show MoreChronic Hepatitis B(CHB) is a serious sequel after Hepatitis B Virus infection, although the rate of chronicity is inverse with age but it may be associated with a significant proportion of deaths related to cirrhosis and liver cancer. HBV serology and measurement of hepatocytic enzymes with viral load can predict disease prognosis and response to treatment. HBeAg refers to a high viral replication and associated with more infectivity to others. This study aimed to determine the impact of HBeAg status in chronically infected patients on various baseline tests. A total of 90 patients with CHB infection who were included in this study. Several standardized measures used for liver function tests, viral load and hepatitis B serological
... Show MoreThis quasi-experimental study investigated generative AI (GenAI) tools—Copilot for chemistry and GitHub Copilot for mathematics—on academic achievement and sustainable professional development among 160 undergraduates (40 experimental/control per department) at the University of Baghdad’s Ibn Al-Haitham College of Education for Pure Sciences (2024–2025). Non-random assignment controlled for covariates. Pre/post validated tests (α ≥ .85; 15 MCQ + 5 essay items) measured outcomes. ANOVA revealed significant gains for experimental groups (p < .001, η2 = .41, Cohen’s d = 0.72 [95% CI: 0.45–0.98]). Chemistry excelled in affective domains; mathematics in cognitive/skills. Findings affirm GenAI’s domain-specific efficacy, prov
... Show MoreThis quasi-experimental study investigated generative AI (GenAI) tools—Copilot for chemistry and GitHub Copilot for mathematics—on academic achievement and sustainable professional development among 160 undergraduates (40 experimental/control per department) at the University of Baghdad’s Ibn Al-Haitham College of Education for Pure Sciences (2024–2025). Non-random assignment controlled for covariates. Pre/post validated tests (α ≥ .85; 15 MCQ + 5 essay items) measured outcomes. ANOVA revealed significant gains for experimental groups (p < .001, η2 = .41, Cohen’s d = 0.72 [95% CI: 0.45–0.98]). Chemistry excelled in affective domains; mathematics in cognitive/skills. Findings affirm GenAI’s domain-specific effica
... Show MoreInflammation of the tonsils could be described as acute tonsillitis, mainly due to infection. Recurrent tonsillitis could be defined as 3-7 episodes during the first 3 years of age. Vitamin D, which is a neuro-hormone with pleiotropic biological activities may modulate the immune response by alleviation, and stimulation of Th1 and Th2 cell proliferation, respectively, that influence the stimulation, synthesis, and secretion of both pro and anti-inflammatory cytokines. In this study we aimed to shed light on the levels of vitamin D in children with different episodes of tonsillitis in association with levels of interleukins (TNFα, IL-2, IL-4, IL-10). Blood samples were collected from 48 participants in 3 groups: control, acute tonsilliti
... Show MoreThis study aims to formulate an alternative solution for Formalin for preserving fish as study specimens for long periods. The main reason for finding a solution instead of formalin is to get rid of the negative effects of this solution on those who work with it, as well as to better preserve the bodies of fish. Hence, three new solutions were proposed to replace formalin. Thus, Formalin, in turn, may enter the composition of a small part of these solutions to give better results and for long periods of keeping specimens. All solutions prepared in this study participated in being acidic as in formalin. Two solutions succeeded in compensating for the use of formalin in preserving fish